2012 Vol. 31, No. 4
column
Display Method:
2012, (4): 1-10.
doi: 10.1007/s13131-012-0215-y
Abstract:
The previous studies by the MASNUM research team have shown the effectiveness of the waveinduced mixing (Bv) in improving the simulation of upper-ocean thermal structure. The mechanisms of Bv are further investigated by incorporating different Bv products into the MASNUM wave-circulation coupled model. First, experiments were designed to explore the effects of Bv, which contain the contributions at different wave lengths (l). The results of three experiments, the non-Bv case, the short-wave case (l <300 m), and the long-wave case (l >300 m) are compared, and it is found that the long waves are the most important component for Bv to generate mixing in the upper ocean. As the swell plays dominant role in mixing, the parameterization of Bv into wind may be not a proper way. Second, Bv effects at different time-scales, including daily and monthly, were examined. The results show that the monthly averaged Bv has larger impact than the daily averaged Bv, especially in summer.
The previous studies by the MASNUM research team have shown the effectiveness of the waveinduced mixing (Bv) in improving the simulation of upper-ocean thermal structure. The mechanisms of Bv are further investigated by incorporating different Bv products into the MASNUM wave-circulation coupled model. First, experiments were designed to explore the effects of Bv, which contain the contributions at different wave lengths (l). The results of three experiments, the non-Bv case, the short-wave case (l <300 m), and the long-wave case (l >300 m) are compared, and it is found that the long waves are the most important component for Bv to generate mixing in the upper ocean. As the swell plays dominant role in mixing, the parameterization of Bv into wind may be not a proper way. Second, Bv effects at different time-scales, including daily and monthly, were examined. The results show that the monthly averaged Bv has larger impact than the daily averaged Bv, especially in summer.
2012, (4): 11-23.
doi: 10.1007/s13131-012-0216-x
Abstract:
The global distributions of eight principal tidal constituents, M2, S2, K1, O1, N2, K2, P1, and Q1, are derived using TOPEX/Poseidon and JASON-1(T/P-J) satellite altimeter data for 16 a. The intercomparison of the derived harmonics at 7000 subsatellite track crossover points shows that the root mean square (RMS) values of the tidal height differences of the above eight constituents range from 1.19 cm to 2.67 cm, with an average of about 2 cm. The RMS values of the tidal height differences between T/P-J solutions and the harmonics from ground measurements at 152 tidal gauge stations for the above constituents range from 0.34 cm to 1.08 cm, and the relative deviations range from 0.031 to 0.211. The root sum square of the RMS differences of these eight constituents is 2.12 cm, showing the improvement of the present model over the existing global ocean tidal models. Based on the obtained tidal model the global ocean tidal energetics is studied and the global distribution of the tidal power input density by tide-generating force of each constituent is calculated, showing that the power input source regions of semidiurnal tides are mainly concentrated in the tropical belt between 30°S and 30°N, while the power input source regions of diurnal tides are mainly concentrated off the tropic oceans. The global energy dissipation rates of the M2, S2, K1, O1, N2, P1, K2 and Q1 tides are 2.424, 0.401, 0.334, 0.160, 0.113, 0.035, 0.030 and 0.006 TW, respectively. The total global tidal dissipation rate of these eight constituents amounts to 3.5 TW.
The global distributions of eight principal tidal constituents, M2, S2, K1, O1, N2, K2, P1, and Q1, are derived using TOPEX/Poseidon and JASON-1(T/P-J) satellite altimeter data for 16 a. The intercomparison of the derived harmonics at 7000 subsatellite track crossover points shows that the root mean square (RMS) values of the tidal height differences of the above eight constituents range from 1.19 cm to 2.67 cm, with an average of about 2 cm. The RMS values of the tidal height differences between T/P-J solutions and the harmonics from ground measurements at 152 tidal gauge stations for the above constituents range from 0.34 cm to 1.08 cm, and the relative deviations range from 0.031 to 0.211. The root sum square of the RMS differences of these eight constituents is 2.12 cm, showing the improvement of the present model over the existing global ocean tidal models. Based on the obtained tidal model the global ocean tidal energetics is studied and the global distribution of the tidal power input density by tide-generating force of each constituent is calculated, showing that the power input source regions of semidiurnal tides are mainly concentrated in the tropical belt between 30°S and 30°N, while the power input source regions of diurnal tides are mainly concentrated off the tropic oceans. The global energy dissipation rates of the M2, S2, K1, O1, N2, P1, K2 and Q1 tides are 2.424, 0.401, 0.334, 0.160, 0.113, 0.035, 0.030 and 0.006 TW, respectively. The total global tidal dissipation rate of these eight constituents amounts to 3.5 TW.
2012, (4): 24-30.
doi: 10.1007/s13131-012-0217-9
Abstract:
In order to determine the design tide levels in the areas without measured tide level data, especially in the areas where it is difficult to measure tidal levels, a calculation method based on a numerical model of tidal current is proposed. The essentials of the method are described, and its application is illustrated with an example. The results of the application show that the design tide levels calculated by the method are close to those determined by long-time measured tide level data, and its calculation precision is high, so it is feasible to use the method to determine the design tide levels in the areas.
In order to determine the design tide levels in the areas without measured tide level data, especially in the areas where it is difficult to measure tidal levels, a calculation method based on a numerical model of tidal current is proposed. The essentials of the method are described, and its application is illustrated with an example. The results of the application show that the design tide levels calculated by the method are close to those determined by long-time measured tide level data, and its calculation precision is high, so it is feasible to use the method to determine the design tide levels in the areas.
2012, (4): 31-45.
doi: 10.1007/s13131-012-0218-8
Abstract:
The sea-surface height (SSH) signatures of internal tides extracted from the TOPEX/Poseidon (T/P) altimeter data along satellite tracks are fitted with superposition of several plane waves which have different wavenumber vectors. The key problem of plane wave fitting with iterative method is how to determine the initial value of wavenumber of each plane wave. The previous solving method is to analyze the internal tidal SSH signatures along each track with wavenumber spectrum. But it is found that the problem cannot be solved completely with the wavenumber spectrum analysis method only. The method based on the combination of wavenumber spectrum analysis method and the exhaustive method is proposed to determine the initial values of wavenumbers for iteration. Numerical results indicate that the proposed method is not only reasonable and feasible but also better than the previous method. The proposed method is an improvement of the previous one, which is beneficial to improving the precision of plane wave fitting of the T/P internal tidal SSH signatures and deepening the understanding of the internal tides in ocean.
The sea-surface height (SSH) signatures of internal tides extracted from the TOPEX/Poseidon (T/P) altimeter data along satellite tracks are fitted with superposition of several plane waves which have different wavenumber vectors. The key problem of plane wave fitting with iterative method is how to determine the initial value of wavenumber of each plane wave. The previous solving method is to analyze the internal tidal SSH signatures along each track with wavenumber spectrum. But it is found that the problem cannot be solved completely with the wavenumber spectrum analysis method only. The method based on the combination of wavenumber spectrum analysis method and the exhaustive method is proposed to determine the initial values of wavenumbers for iteration. Numerical results indicate that the proposed method is not only reasonable and feasible but also better than the previous method. The proposed method is an improvement of the previous one, which is beneficial to improving the precision of plane wave fitting of the T/P internal tidal SSH signatures and deepening the understanding of the internal tides in ocean.
2012, (4): 46-54.
doi: 10.1007/s13131-012-0219-7
Abstract:
The warming of the Arctic Intermediate Water (AIW) is studied based on the analyses of hydrographic observations in the Canada Basin of the Arctic Ocean during 1985-2006. It is shown that how the anomalously warm AIW spreads in the Canada Basin during the observation time through the analysis of the AIW temperature spatial distribution in different periods. The results indicate that by 2006, the entire Canada Basin has almost been covered by the warming AIW. In order to study interannual variability of the AIW in the Canada Basin, the Canada Basin is divided into five regions according to the bottom topography. From the interannual variation of AIW temperature in each region, it is shown that a cooling period follows after the warming event in upstream regions. At the Chukchi Abyssal Plain and Chukchi Plateau, upstream of the Arctic Circumpolar Boundary Current (ACBC) in the Canada Basin, the AIW temperature reached maximum and then started to fall respectively in 2000 and 2002. However, the AIW in the Canada Abyssal Plain and Beaufort Sea continues to warm monotonically until the year 2006. Furthermore, it is revealed that there is convergence of the AIW depth in the five different regions of the Canada Basin when the AIW warming occurs during observation time. The difference of AIW depth between the five regions of the Canada Basin is getting smaller and smaller, all approaching 410 m in recent years. The results show that depth convergence is related to the variation of AIW potential density in the Canada Basin.
The warming of the Arctic Intermediate Water (AIW) is studied based on the analyses of hydrographic observations in the Canada Basin of the Arctic Ocean during 1985-2006. It is shown that how the anomalously warm AIW spreads in the Canada Basin during the observation time through the analysis of the AIW temperature spatial distribution in different periods. The results indicate that by 2006, the entire Canada Basin has almost been covered by the warming AIW. In order to study interannual variability of the AIW in the Canada Basin, the Canada Basin is divided into five regions according to the bottom topography. From the interannual variation of AIW temperature in each region, it is shown that a cooling period follows after the warming event in upstream regions. At the Chukchi Abyssal Plain and Chukchi Plateau, upstream of the Arctic Circumpolar Boundary Current (ACBC) in the Canada Basin, the AIW temperature reached maximum and then started to fall respectively in 2000 and 2002. However, the AIW in the Canada Abyssal Plain and Beaufort Sea continues to warm monotonically until the year 2006. Furthermore, it is revealed that there is convergence of the AIW depth in the five different regions of the Canada Basin when the AIW warming occurs during observation time. The difference of AIW depth between the five regions of the Canada Basin is getting smaller and smaller, all approaching 410 m in recent years. The results show that depth convergence is related to the variation of AIW potential density in the Canada Basin.
2012, (4): 55-64.
doi: 10.1007/s13131-012-0220-1
Abstract:
Applying the methods of on-site observation and dynamic model, the research on the fronts at the Jiulong Estuary has been carried out, during which spatial and temporal distribution, dynamic characteristics and formation mechanism of salinity fronts are analyzed and discussed. The research shows that the estuarine fronts mainly lie in the area from the Jiyu Islet to the Haimen Island, outside of Yuweizai to Hulishan cross-section, the near coast of Yuweizai and the south of the Songyu-Gulangyu Channel. The fronts in the former two regions are formed directly by plume, while the one near the coast of Yuweizai is a tidal intrusion front caused by flood current and the one at the south of the Songyu-Gulangyu Channel is the result of current shear transformation. Under normal circumstances, fresh water of the Jiulong River mainly influences the inside of the Xiamen Bay, and when it is in typhoon seasons, plume front can affect the Taiwan Strait and has an effect on the biogeochemical processes in the strait.
Applying the methods of on-site observation and dynamic model, the research on the fronts at the Jiulong Estuary has been carried out, during which spatial and temporal distribution, dynamic characteristics and formation mechanism of salinity fronts are analyzed and discussed. The research shows that the estuarine fronts mainly lie in the area from the Jiyu Islet to the Haimen Island, outside of Yuweizai to Hulishan cross-section, the near coast of Yuweizai and the south of the Songyu-Gulangyu Channel. The fronts in the former two regions are formed directly by plume, while the one near the coast of Yuweizai is a tidal intrusion front caused by flood current and the one at the south of the Songyu-Gulangyu Channel is the result of current shear transformation. Under normal circumstances, fresh water of the Jiulong River mainly influences the inside of the Xiamen Bay, and when it is in typhoon seasons, plume front can affect the Taiwan Strait and has an effect on the biogeochemical processes in the strait.
2012, (4): 65-75.
doi: 10.1007/s13131-012-0221-0
Abstract:
Sea-ice physical characteristics were investigated in the Arctic section of 143°-180°W during August and early September 2008. Ship-based observations show that both the sea-ice thickness and concentration recorded during southward navigation from 30 August to 6 September were remarkably less than those recorded during northward navigation from 3 to 30 August, especially at low latitudes. Accordingly, the marginal ice zone moved from about 74.0°N to about 79.5°N from mid-August to early September. Melt-pond coverage increased with increasing latitude, peaking at 84.4°N, where about 27% of ice was covered by melt ponds. Above this latitude, melt-pond coverage decreased evidently as the ice at high latitudes experienced a relatively short melt season and commenced its growth stage by the end of August. Regional mean ice thickness increased from 0.8 (±0.5) m at 75.0°N to 1.5 (±0.4) m at 85.0°N along the northward navigation while it decreased rapidly to 0.6 (±0.3) m at 78.0°N along the southward navigation. Because of relatively low ice concentration and thin ice in the investigated Arctic sector, both the short-term ice stations and ice camp could only be set up over multiyear sea ice. Observations of ice properties based on ice cores collected at the short-term ice stations and the ice camp show that all investigated floes were essentially isothermal with high temperature and porosity, and low density and salinity. Most ices had salinity below 2 and mean density of 800-860 kg/m3. Significant ice loss in the investigated Arctic sector during the last 15 a can be identified by comparison with the previous observations.
Sea-ice physical characteristics were investigated in the Arctic section of 143°-180°W during August and early September 2008. Ship-based observations show that both the sea-ice thickness and concentration recorded during southward navigation from 30 August to 6 September were remarkably less than those recorded during northward navigation from 3 to 30 August, especially at low latitudes. Accordingly, the marginal ice zone moved from about 74.0°N to about 79.5°N from mid-August to early September. Melt-pond coverage increased with increasing latitude, peaking at 84.4°N, where about 27% of ice was covered by melt ponds. Above this latitude, melt-pond coverage decreased evidently as the ice at high latitudes experienced a relatively short melt season and commenced its growth stage by the end of August. Regional mean ice thickness increased from 0.8 (±0.5) m at 75.0°N to 1.5 (±0.4) m at 85.0°N along the northward navigation while it decreased rapidly to 0.6 (±0.3) m at 78.0°N along the southward navigation. Because of relatively low ice concentration and thin ice in the investigated Arctic sector, both the short-term ice stations and ice camp could only be set up over multiyear sea ice. Observations of ice properties based on ice cores collected at the short-term ice stations and the ice camp show that all investigated floes were essentially isothermal with high temperature and porosity, and low density and salinity. Most ices had salinity below 2 and mean density of 800-860 kg/m3. Significant ice loss in the investigated Arctic sector during the last 15 a can be identified by comparison with the previous observations.
2012, (4): 76-88.
doi: 10.1007/s13131-012-0222-z
Abstract:
The response of chlorophyll a (Chl a) concentration to wind stress is analyzed in the South China Sea (SCS), using in-situ data of Chl a and remote sensing data (QuikScat-sea surface wind (SSW), AVHRR-sea surface temperature (SST), AVISO merged-sea level anomalies (SLA), SeaWiFSderived Chl a and MODIS Terra-derived Chl a) in August/September/October 2004, 2006 and 2009. The variability of SSW, SST and SLA 7 d before in-situ Chl a sampling (including the work day of in-situ Chl a sampling) with the same latitude and longitude of the study area are investigated, and the correlation coefficients are calculated between these hydrographic factors and in-situ Chl a concentration. The results show that the Chl a-SSW correlation coefficients at upper layers (such as 0 m and 25 m) are more significant than those at deeper layers (such as 50, 75 and 100 m) 1-3 d before, which indicates that there is a time lag of strong surface winds stimulating phytoplankton bloom. By analyzing the relationship among the daily remote sensing derived (RSderived) SSW, SST, SLA and 3 d averaged SeaWiFS/MODIS-derived Chl a concentration in the northern SCS in September 2004 and 2009 respectively, it shows that the intensity and speed of surface winds could have great influence on extend of Chl a increase. If surface winds reach 4-5 m/s over, Chl a concentration would increase 1-3 d after the process of strong surface winds in open sea area of the northern SCS mainly during September.
The response of chlorophyll a (Chl a) concentration to wind stress is analyzed in the South China Sea (SCS), using in-situ data of Chl a and remote sensing data (QuikScat-sea surface wind (SSW), AVHRR-sea surface temperature (SST), AVISO merged-sea level anomalies (SLA), SeaWiFSderived Chl a and MODIS Terra-derived Chl a) in August/September/October 2004, 2006 and 2009. The variability of SSW, SST and SLA 7 d before in-situ Chl a sampling (including the work day of in-situ Chl a sampling) with the same latitude and longitude of the study area are investigated, and the correlation coefficients are calculated between these hydrographic factors and in-situ Chl a concentration. The results show that the Chl a-SSW correlation coefficients at upper layers (such as 0 m and 25 m) are more significant than those at deeper layers (such as 50, 75 and 100 m) 1-3 d before, which indicates that there is a time lag of strong surface winds stimulating phytoplankton bloom. By analyzing the relationship among the daily remote sensing derived (RSderived) SSW, SST, SLA and 3 d averaged SeaWiFS/MODIS-derived Chl a concentration in the northern SCS in September 2004 and 2009 respectively, it shows that the intensity and speed of surface winds could have great influence on extend of Chl a increase. If surface winds reach 4-5 m/s over, Chl a concentration would increase 1-3 d after the process of strong surface winds in open sea area of the northern SCS mainly during September.
2012, (4): 89-100.
doi: 10.1007/s13131-012-0223-y
Abstract:
The Inductively Coupled Plasma Optical Emission Spectrometer was used to analyze sediment samples collected from the Hangzhou Bay to determine major and trace elemental concentrations. Based on these concentrations, the study area can be classified into three geochemical provinces. Province I covers the northern Hangzhou Bay area and contains high concentrations of Al2O3, Fe2O3, MgO, Na2O, K2O, MnO, Cr, Cu, Ni, Pb, V, Co and Zn. Province Ⅲ is located in the western Hangzhou Bay, near the Qiantang River mouth, and contains high concentrations of SiO2, Na2O, P2O5, TiO2, Cr, Sr, Zr. Province Ⅱ is located in the middle and eastern Hangzhou Bay, with the medium concentrations of major and trace elements. The results also demonstrate that the grain size is the dominating factor controlling the spatial variations of elemental concentrations, and the Changjiang River (Yangtze River) and Qiantang River sediments play an important role in the distribution of these elements. Anthropogenic impact on heavy metal concentrations (especially Cr, Sr and Zr) can be detected in the surface sediments near the Qiantang River mouth.
The Inductively Coupled Plasma Optical Emission Spectrometer was used to analyze sediment samples collected from the Hangzhou Bay to determine major and trace elemental concentrations. Based on these concentrations, the study area can be classified into three geochemical provinces. Province I covers the northern Hangzhou Bay area and contains high concentrations of Al2O3, Fe2O3, MgO, Na2O, K2O, MnO, Cr, Cu, Ni, Pb, V, Co and Zn. Province Ⅲ is located in the western Hangzhou Bay, near the Qiantang River mouth, and contains high concentrations of SiO2, Na2O, P2O5, TiO2, Cr, Sr, Zr. Province Ⅱ is located in the middle and eastern Hangzhou Bay, with the medium concentrations of major and trace elements. The results also demonstrate that the grain size is the dominating factor controlling the spatial variations of elemental concentrations, and the Changjiang River (Yangtze River) and Qiantang River sediments play an important role in the distribution of these elements. Anthropogenic impact on heavy metal concentrations (especially Cr, Sr and Zr) can be detected in the surface sediments near the Qiantang River mouth.
2012, (4): 101-112.
doi: 10.1007/s13131-012-0224-x
Abstract:
To investigate the impacts of nutrient concentrations and N:P:Si ratios on the ecosystem of the Huanghai Sea (Yellow Sea), the current status and long-term variation of nutrients concentrations and ratios as well as phytoplankton community structure in the Huanghai Sea were collected and analyzed. The results reveal great annual and seasonal fluctuations in the nutrient concentrations and N:P:Si ratios during 1998-2008 with no clear pattern observed in the whole region. Yet on a seasonal scale of spring and in the coastal regions such as the Jiaozhou Bay and Sanggou Bay, the increase of DIN concentration and N:P ratio as well as the decrease of phosphate and silicate concentrations and Si:N ratios were relatively significant. Many pelagic ecosystem changes have occurred concurrent with these changes of the nutrient regime, such as the recent increase of primary production, changes of phytoplankton chlorophyll a biomass and abundance, an increase of eutrophication, and occurrence of HABs. In addition, new trends in the variation of nutrients seem to be developing in some particular transect such as 36°N, which suggests that long-term and systematic ecosystem monitoring in the Huanghai Sea is necessary.
To investigate the impacts of nutrient concentrations and N:P:Si ratios on the ecosystem of the Huanghai Sea (Yellow Sea), the current status and long-term variation of nutrients concentrations and ratios as well as phytoplankton community structure in the Huanghai Sea were collected and analyzed. The results reveal great annual and seasonal fluctuations in the nutrient concentrations and N:P:Si ratios during 1998-2008 with no clear pattern observed in the whole region. Yet on a seasonal scale of spring and in the coastal regions such as the Jiaozhou Bay and Sanggou Bay, the increase of DIN concentration and N:P ratio as well as the decrease of phosphate and silicate concentrations and Si:N ratios were relatively significant. Many pelagic ecosystem changes have occurred concurrent with these changes of the nutrient regime, such as the recent increase of primary production, changes of phytoplankton chlorophyll a biomass and abundance, an increase of eutrophication, and occurrence of HABs. In addition, new trends in the variation of nutrients seem to be developing in some particular transect such as 36°N, which suggests that long-term and systematic ecosystem monitoring in the Huanghai Sea is necessary.
2012, (4): 113-126.
doi: 10.1007/s13131-012-0225-9
Abstract:
Our laboratory study concerns the competitive interaction between two marine rotifer species Brachionus plicatilis and Brachionus rotundiformis at five algal (Nannochloropsis salina) concentrations (0.4×106 to 32.4×106 cells/ml) and at four initial inoculation densities (numerically, 100% B. plicatilis; 75% B. plicatilis and 25% B. rotundiformis, 50% each of the two species; 25% B. plicatilis and 75% B. rotundiformis and 100% B. rotundiformis. The initial biomass varied as 0.33 and 0.22 μg/ml for B. plicatilis and B. rotundiformis respectively. Experiments were performed at (25±1)℃. Population densities were enumerated and the medium was changed daily up to 8 d in the experiment. At the lowest food level tested, B. rotundiformis formed superior competitor than B. plicatilis, regardless of starting inoculation density. Generally when the food concentrations increased, B. plicatilis showed a greater increase in biomass than B. rotundiformis. B. rotundiformis formed the largest in population growth, regardless of increasing food concentrations. When grown alone, B. plicatilis reached peak abundances of (1.311 5±0.028) and (137.5±0.014) μg/ml at low and high food densities respectively. The corresponding values of B. rotundiformis were 0.724 5±0.016 and 18.15±0.021. The adverse effects of B. rotundiformis on the peak abundances of B. plicatilis were observed at the lowest food level and higher initial density. The rate of population growth in controls varied from (0.792±0.162) to (1.482±0.132) μm/d for B. plicatilis and (0.445±0.041) to (0.856±0.012) μm/d for B. rotundiformis, depending on food level. When both species were introduced together, low food levels favoured higher abundance of B. rotundiformis than B. plicatilis, suggesting that increased population density of the smaller B. rotundiformis was more successfull than larger B. plicatilis in brackish waters. Our work reveals that available food (type and quantity) along with starting inoculation density had significant effect on the interspecific competition between marine sibling rotifer species in zooplankton community structure.
Our laboratory study concerns the competitive interaction between two marine rotifer species Brachionus plicatilis and Brachionus rotundiformis at five algal (Nannochloropsis salina) concentrations (0.4×106 to 32.4×106 cells/ml) and at four initial inoculation densities (numerically, 100% B. plicatilis; 75% B. plicatilis and 25% B. rotundiformis, 50% each of the two species; 25% B. plicatilis and 75% B. rotundiformis and 100% B. rotundiformis. The initial biomass varied as 0.33 and 0.22 μg/ml for B. plicatilis and B. rotundiformis respectively. Experiments were performed at (25±1)℃. Population densities were enumerated and the medium was changed daily up to 8 d in the experiment. At the lowest food level tested, B. rotundiformis formed superior competitor than B. plicatilis, regardless of starting inoculation density. Generally when the food concentrations increased, B. plicatilis showed a greater increase in biomass than B. rotundiformis. B. rotundiformis formed the largest in population growth, regardless of increasing food concentrations. When grown alone, B. plicatilis reached peak abundances of (1.311 5±0.028) and (137.5±0.014) μg/ml at low and high food densities respectively. The corresponding values of B. rotundiformis were 0.724 5±0.016 and 18.15±0.021. The adverse effects of B. rotundiformis on the peak abundances of B. plicatilis were observed at the lowest food level and higher initial density. The rate of population growth in controls varied from (0.792±0.162) to (1.482±0.132) μm/d for B. plicatilis and (0.445±0.041) to (0.856±0.012) μm/d for B. rotundiformis, depending on food level. When both species were introduced together, low food levels favoured higher abundance of B. rotundiformis than B. plicatilis, suggesting that increased population density of the smaller B. rotundiformis was more successfull than larger B. plicatilis in brackish waters. Our work reveals that available food (type and quantity) along with starting inoculation density had significant effect on the interspecific competition between marine sibling rotifer species in zooplankton community structure.
2012, (4): 127-139.
doi: 10.1007/s13131-012-0226-8
Abstract:
Samples were collected with a plankton net in the four seasonal cruises during 2006-2007 to study the seasonal variability of the zooplankton community in the southwest part of Huanghai Sea Cold Water Mass (HSCWM,Yellow Sea Cold Water Mass).The spatial and temporal variations of zooplankton species composition,biomass,abundance and biodiversity were examined.A total of 122 zooplankton species and 30 pelagic larvae were identified in the four cruises.Calanus sinicus and Aidanosagitta crassa were the most dominant species,and Themisto gaudichaudi and Euphausia pacifica were widely distributed in the HSCWM area.The spatial patterns of non-gelatinous zooplankton (removing the high water content groups) were similar to those of the total zooplankton biomass in autumn,but different significantly in the other three seasons.The seasonal means of zooplankton biomass in spring and summer were much higher than that in autumn and winter.The total zooplankton abundance averaged 283.5 ind./m3 in spring (highest),192.5 ind./m3 in summer,165.5 ind./m3 in autumn and 65.9 ind./m3 in winter (lowest),and the non-gelatinous groups contributed the most total abundance.Correlation analysis suggests that the non-gelatinous zooplankton biomass and abundance had a significant positive correlation in the whole year,but the relationship was insignificant between the total zooplankton biomass and abundance in spring and summer.The diversity index H' of zooplankton community averaged 1.88 in this study,which was somewhat higher than historical results.Relatively low diversity in summer was related to the high dominance of Calanus sinicus,probably due to the strongest effect of the HSCWM in this season.
Samples were collected with a plankton net in the four seasonal cruises during 2006-2007 to study the seasonal variability of the zooplankton community in the southwest part of Huanghai Sea Cold Water Mass (HSCWM,Yellow Sea Cold Water Mass).The spatial and temporal variations of zooplankton species composition,biomass,abundance and biodiversity were examined.A total of 122 zooplankton species and 30 pelagic larvae were identified in the four cruises.Calanus sinicus and Aidanosagitta crassa were the most dominant species,and Themisto gaudichaudi and Euphausia pacifica were widely distributed in the HSCWM area.The spatial patterns of non-gelatinous zooplankton (removing the high water content groups) were similar to those of the total zooplankton biomass in autumn,but different significantly in the other three seasons.The seasonal means of zooplankton biomass in spring and summer were much higher than that in autumn and winter.The total zooplankton abundance averaged 283.5 ind./m3 in spring (highest),192.5 ind./m3 in summer,165.5 ind./m3 in autumn and 65.9 ind./m3 in winter (lowest),and the non-gelatinous groups contributed the most total abundance.Correlation analysis suggests that the non-gelatinous zooplankton biomass and abundance had a significant positive correlation in the whole year,but the relationship was insignificant between the total zooplankton biomass and abundance in spring and summer.The diversity index H' of zooplankton community averaged 1.88 in this study,which was somewhat higher than historical results.Relatively low diversity in summer was related to the high dominance of Calanus sinicus,probably due to the strongest effect of the HSCWM in this season.
2012, (4): 140-148.
doi: 10.1007/s13131-012-0227-7
Abstract:
Edwardsiella tarda is one of the most important emerging pathogens in the global aquaculture industries. As such, an accurate diagnosis and quantitative analytical methods are urgently needed for this bacterium. In this study, primers and a TaqMan probe specific to the conservative sequences of the 16S rRNA gene of E. tarda were designed. The concentration of primers and TaqMan probe were optimized to 200 nmol/L and 120 nmol/L, respectively. The detection sensitivity of the FQPCR assay was determined to be as low as five copies of the target sequence per reaction using the pGEM-16S rDNA recombinant plasmid as a template, which was 100 times more sensitive than conventional PCR. A standard curve by plotting the threshold cycle values (y) against the common logarithmic copies (log10nc as x; nc is copy number) of pGEM-16S rDNA was generated. The results of intra-and inter-assay variability tests demonstrate that the established FQ-PCR method was highly reproducible. The assay was specific for E. tarda as it showed that there was no cross-reactivity to eight additional bacterial pathogen strains in aquaculture. Thus, the FQ-PCR assay has the potential for diagnostic purposes and for other applications, especially for the rapid detection and quantification of low-grade E. tarda infections.
Edwardsiella tarda is one of the most important emerging pathogens in the global aquaculture industries. As such, an accurate diagnosis and quantitative analytical methods are urgently needed for this bacterium. In this study, primers and a TaqMan probe specific to the conservative sequences of the 16S rRNA gene of E. tarda were designed. The concentration of primers and TaqMan probe were optimized to 200 nmol/L and 120 nmol/L, respectively. The detection sensitivity of the FQPCR assay was determined to be as low as five copies of the target sequence per reaction using the pGEM-16S rDNA recombinant plasmid as a template, which was 100 times more sensitive than conventional PCR. A standard curve by plotting the threshold cycle values (y) against the common logarithmic copies (log10nc as x; nc is copy number) of pGEM-16S rDNA was generated. The results of intra-and inter-assay variability tests demonstrate that the established FQ-PCR method was highly reproducible. The assay was specific for E. tarda as it showed that there was no cross-reactivity to eight additional bacterial pathogen strains in aquaculture. Thus, the FQ-PCR assay has the potential for diagnostic purposes and for other applications, especially for the rapid detection and quantification of low-grade E. tarda infections.
2012, (4): 149-153.
doi: 10.1007/s13131-012-0228-6
Abstract:
An (AC)n-microsatellite-enriched library for Larimichthys crocea was constructed in this study. Primers for fifty simple sequence repeat (SSR) loci were synthesized and genotyped on 30 L. crocea individuals from Guanjingyang wild population (WP) in Fujian Province and 38 individuals from Ningbo cultured population (CP) in Zhejiang Province. Only 21 loci were successfully amplified and polymorphic in two populations. In WP, the observed heterozygosity (HO) ranged from 0.233 to 0.900 and the expected heterozygosity (HE) ranged from 0.326 to 0.893, with an average of 7.8 alleles/locus, the polymorphism information content (PIC) ranged from 0.283 to 0.866 (mean 0.731). In CP, the HO ranged from 0.189 to 0.892 and the HE ranged from 0.333 to 0.800, with an average of 4.4 alleles/locus. The probability test showed significant departures from HWE in 9 and 2 of the 21 loci in WP and in CP, respectively. Deficiency of heterozygotes at four loci showed the presence of null alleles (P <0.01). The PIC of 20 microsatellite loci in WP were greater than 0.50. Overall, these novel markers are potentially useful for the study of population genetics, construction of genetic linkage and quantitative trait loci maps in large yellow croaker.
An (AC)n-microsatellite-enriched library for Larimichthys crocea was constructed in this study. Primers for fifty simple sequence repeat (SSR) loci were synthesized and genotyped on 30 L. crocea individuals from Guanjingyang wild population (WP) in Fujian Province and 38 individuals from Ningbo cultured population (CP) in Zhejiang Province. Only 21 loci were successfully amplified and polymorphic in two populations. In WP, the observed heterozygosity (HO) ranged from 0.233 to 0.900 and the expected heterozygosity (HE) ranged from 0.326 to 0.893, with an average of 7.8 alleles/locus, the polymorphism information content (PIC) ranged from 0.283 to 0.866 (mean 0.731). In CP, the HO ranged from 0.189 to 0.892 and the HE ranged from 0.333 to 0.800, with an average of 4.4 alleles/locus. The probability test showed significant departures from HWE in 9 and 2 of the 21 loci in WP and in CP, respectively. Deficiency of heterozygotes at four loci showed the presence of null alleles (P <0.01). The PIC of 20 microsatellite loci in WP were greater than 0.50. Overall, these novel markers are potentially useful for the study of population genetics, construction of genetic linkage and quantitative trait loci maps in large yellow croaker.
2012, (4): 154-160.
doi: 10.1007/s13131-012-0229-5
Abstract:
Generally, nutrient cycle is closely related to the element distribution in biomass and the population dynamics in ecosystems. Carbon, nitrogen and phosphorus in Japanese anchovy (Engraulis japonicus) of different body lengths from the Huanghai Sea (Yellow Sea) were determined to better understand their variability and reasons during its life history. The mean content was 45.12%, 10.12% and 2.02% for C, N and P, respectively. Significant differences in C, N and P elemental composition were found among different sizes, which could be explained by varying proportions of storage compounds in whole body fish, and varying degrees of ossification. Considering abundant resources in Japanese anchovy, it was an important P-pool in the cycle of P. Moreover, the excreted N/P ratio was significantly different in fishes of different sizes, especially at high gross growth efficiency. In the past two decades, overexploitation tended to cause smaller body length in the community structure of anchovy, which presumably changed the nutrient cycle in food webs of the Huanghai Sea. Exptrapolation of the results indicates that Japanese anchovy may be important for conveying nutrient in the Huanghai Sea.
Generally, nutrient cycle is closely related to the element distribution in biomass and the population dynamics in ecosystems. Carbon, nitrogen and phosphorus in Japanese anchovy (Engraulis japonicus) of different body lengths from the Huanghai Sea (Yellow Sea) were determined to better understand their variability and reasons during its life history. The mean content was 45.12%, 10.12% and 2.02% for C, N and P, respectively. Significant differences in C, N and P elemental composition were found among different sizes, which could be explained by varying proportions of storage compounds in whole body fish, and varying degrees of ossification. Considering abundant resources in Japanese anchovy, it was an important P-pool in the cycle of P. Moreover, the excreted N/P ratio was significantly different in fishes of different sizes, especially at high gross growth efficiency. In the past two decades, overexploitation tended to cause smaller body length in the community structure of anchovy, which presumably changed the nutrient cycle in food webs of the Huanghai Sea. Exptrapolation of the results indicates that Japanese anchovy may be important for conveying nutrient in the Huanghai Sea.
