The concentration and partition ratio of various speciations of nutrients in domestic sewage were determined.The transformation and transportation among the speciations,as well as their biological effects during sewage-seawater mixing were simulated in laboratory.The results are compared with field observation and the following findings are:ⅰ) The suitable range of ratio DIN/DIP in seawater for growth of phytoplankton in subtropical estuary and harbor is quite wide.It could grow well even in the range of 15-55 in atom ratio,and is independ of the variation in levels of N and P.ⅱ) The transformation rate among the speciations of phosphorus is within the range of 0.5 to 1.1 μmol/d.ⅲ) Phytoplankton uptakes P prior to N during its growth.The growth rate for Skeletonema costatum (the major species) and field algae are 0.34-0.58/d and 0.30-0.31/d,respectively.ⅳ) The red tide species Prorocentrum micans could become the dominant species to a density 107 cells/L after Skeletonema costatum blooms and phosphorus is almost exhausted.