Transcriptome-wide evolutionary analysis on essential brown algae (<i>Phaeophyceae</i>) in China

SUN Jing; WANG Liang; WU Shuangxiu; WANG Xumin; XIAO Jingfa; CHI Shan; LIU Cui; REN Lufeng; ZHAO Yuhui; LIU Tao; YU Jun

doi:10.1007/s13131-014-0436-3

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Article Navigation > Acta Oceanologica Sinica > 2014 > 33(2): 13-19

SUN Jing, WANG Liang, WU Shuangxiu, WANG Xumin, XIAO Jingfa, CHI Shan, LIU Cui, REN Lufeng, ZHAO Yuhui, LIU Tao, YU Jun. Transcriptome-wide evolutionary analysis on essential brown algae (Phaeophyceae) in China[J]. Acta Oceanologica Sinica, 2014, 33(2): 13-19. doi: 10.1007/s13131-014-0436-3

Citation:

SUN Jing, WANG Liang, WU Shuangxiu, WANG Xumin, XIAO Jingfa, CHI Shan, LIU Cui, REN Lufeng, ZHAO Yuhui, LIU Tao, YU Jun. Transcriptome-wide evolutionary analysis on essential brown algae (Phaeophyceae) in China[J]. Acta Oceanologica Sinica, 2014, 33(2): 13-19. doi: 10.1007/s13131-014-0436-3

Citation:

SUN Jing, WANG Liang, WU Shuangxiu, WANG Xumin, XIAO Jingfa, CHI Shan, LIU Cui, REN Lufeng, ZHAO Yuhui, LIU Tao, YU Jun. Transcriptome-wide evolutionary analysis on essential brown algae (Phaeophyceae) in China[J]. Acta Oceanologica Sinica, 2014, 33(2): 13-19. doi: 10.1007/s13131-014-0436-3

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Transcriptome-wide evolutionary analysis on essential brown algae (Phaeophyceae) in China

doi: 10.1007/s13131-014-0436-3

1.
CAS Key Laboratory of Genome Sciences and Information, Beijing Key Laboratory of Genome and Precision Medicine Technologies, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China;Beijing Key Laboratory of Functional Genomics for Dao-di Herbs, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China;University of Chinese Academy of Sciences, Beijing 100049, China
2.
CAS Key Laboratory of Genome Sciences and Information, Beijing Key Laboratory of Genome and Precision Medicine Technologies, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China;Beijing Key Laboratory of Functional Genomics for Dao-di Herbs, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China
3.
College of Marine Life Science, Ocean University of China, Qingdao 266003, China
4.
CAS Key Laboratory of Genome Sciences and Information, Beijing Key Laboratory of Genome and Precision Medicine Technologies, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China;University of Chinese Academy of Sciences, Beijing 100049, China

Received Date: 2013-04-01
Rev Recd Date: 2013-07-18

Abstract

Abstract

Brown algae (Chromista, Ochrophyta, Phaeophyceae) are a large group of multicellular algae that play important roles in the ocean's ecosystem and biodiversity. However, poor molecular bases for studying their phylogenetic evolutions and novel metabolic characteristics have hampered progress in the field. In this study, we sequenced the de novo transcriptome of 18 major species of brown algae in China, covering six orders and seven families, using the high-throughput sequencing platform Illumina HiSeq 2000. From the transcriptome data of these 18 species and publicly available genome data of Ectocarpus siliculosus and Phaeodactylum tricornutum, we identified 108 nuclear-generated orthologous genes and clarified the phylogenetic relationships among these brown algae based on a multigene method. These brown algae could be separated into two clades: Clade Ishigeales-Dictyotales and Clade Ectocarpales-Laminariales-Desmarestiale-Fucales. The former was at the base of the phylogenetic tree, indicating its early divergence, while the latter was divided into two branches, with Order Fucales diverging from Orders Ectocarpales, Laminariales, and Desmarestiale. In our analysis of taxonomy-contentious species, Sargassum fusiforme and Saccharina sculpera were found to be closely related to genera Sargassum and Saccharina, respectively, while Petalonia fascia showed possible relation to genus Scytosiphon. The study provided molecular evidence for the phylogenetic taxonomy of brown algae.
- Phaeophyceae,
- transcriptome sequencing,
- multigene,
- phylogeny

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References(37)

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