Antimicrobial peptide hepcidin contributes to host defense of <i>Centropristis striata</i> against <i>Vibrio harveyi</i> challenge

Xiaowan Ma; Ying Qiao; Yanxiang Shao; Chao Chen

doi:10.1007/s13131-021-1776-4

Volume 40 Issue 6

Jun. 2021

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Xiaowan Ma, Ying Qiao, Yanxiang Shao, Chao Chen. Antimicrobial peptide hepcidin contributes to host defense of Centropristis striata against Vibrio harveyi challenge[J]. Acta Oceanologica Sinica, 2021, 40(6): 61-66. doi: 10.1007/s13131-021-1776-4

Citation:

Xiaowan Ma, Ying Qiao, Yanxiang Shao, Chao Chen. Antimicrobial peptide hepcidin contributes to host defense of Centropristis striata against Vibrio harveyi challenge[J]. Acta Oceanologica Sinica, 2021, 40(6): 61-66. doi: 10.1007/s13131-021-1776-4

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Antimicrobial peptide hepcidin contributes to host defense of Centropristis striata against Vibrio harveyi challenge

doi: 10.1007/s13131-021-1776-4

1.
Key Laboratory of Tropical Marine Ecosystem and Bioresource, Fourth Institute of Oceanography, Ministry of Natural Resources, Beihai 536000, China
2.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China

Funds: The Youth Foundation of Guangxi Zhuang Autonomous Region, China under contract No. 2018GXNSFBA050032; the Innovation Driven Development Foundation of Guangxi under contract Nos AD19245135 and AD19245161; the Doctoral Research Startup Fund of the Fourth Institute of Oceanography, Ministry of Natural Resources under contract Nos 201803 and 201806; the Fund of Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, China under contract No. FREU2016-04.

More Information

Corresponding author: E-mail: qiaoying0618@hotmail.com
Received Date: 2020-04-29
Accepted Date: 2020-06-03

Available Online: 2021-06-29

Publish Date: 2021-06-01

Abstract

Abstract

Hepcidins are small cysteine-rich antimicrobial peptides that play a vital role in immunity against pathogen invasion. Here, a hepcidin (Cshep) from Centropristis striata was described, which is considered as a valuable aquaculture marine species in China. The open reading frame consisted of 273 bp. Eight conserved cysteine residues were identified. Phylogenetic analysis showed that Cshep had a relatively close relationship with the hepcidin from Epinephelus moara. Quantitative real-time PCR analysis demonstrated that Cshep was highly expressed in liver and significantly up-regulated when challenged with Vibrio harveyi. In addition, the synthetic Cshep peptide had a high antimicrobial activity against V. harveyi, but low against other pathogenic bacteria tested in this study. The killing kinetics analysis revealed that Cshep had a fast bactericidal effect on V. harveyi. These results suggested that Cshep may be involved in the immune response of C. striata against V. harveyi infection.
- Centropristis striata,
- hepcidin,
- antimicrobial peptides,
- antimicrobial activity,
- Vibrio harveyi

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References(27)

References

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