Isolation, distribution and evaluation of cytotoxic and antioxidant activity of cultivable actinobacteria from the Oman Sea sediments

Gozari Mohsen; Bahador Nima; Jassbi Amir Reza; Mortazavi Mohammad Seddiq; Hamzehei Samad; Eftekhar Ebrahim

doi:10.1007/s13131-019-1515-2

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Gozari Mohsen, Bahador Nima, Jassbi Amir Reza, Mortazavi Mohammad Seddiq, Hamzehei Samad, Eftekhar Ebrahim. Isolation, distribution and evaluation of cytotoxic and antioxidant activity of cultivable actinobacteria from the Oman Sea sediments[J]. Acta Oceanologica Sinica, 2019, 38(12): 84-90. doi: 10.1007/s13131-019-1515-2

Citation:

Gozari Mohsen, Bahador Nima, Jassbi Amir Reza, Mortazavi Mohammad Seddiq, Hamzehei Samad, Eftekhar Ebrahim. Isolation, distribution and evaluation of cytotoxic and antioxidant activity of cultivable actinobacteria from the Oman Sea sediments[J]. Acta Oceanologica Sinica, 2019, 38(12): 84-90. doi: 10.1007/s13131-019-1515-2

Citation:

Gozari Mohsen, Bahador Nima, Jassbi Amir Reza, Mortazavi Mohammad Seddiq, Hamzehei Samad, Eftekhar Ebrahim. Isolation, distribution and evaluation of cytotoxic and antioxidant activity of cultivable actinobacteria from the Oman Sea sediments[J]. Acta Oceanologica Sinica, 2019, 38(12): 84-90. doi: 10.1007/s13131-019-1515-2

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Isolation, distribution and evaluation of cytotoxic and antioxidant activity of cultivable actinobacteria from the Oman Sea sediments

doi: 10.1007/s13131-019-1515-2

1.
Department of Microbiology, College of Science, Agriculture and Modern Technology, Shiraz Branch, Islamic Azad University, Shiraz 71331, Iran;Persian Gulf and Oman Sea Ecological Research Center, Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO), Bandar Abbas 79131, Iran
2.
Department of Microbiology, College of Science, Agriculture and Modern Technology, Shiraz Branch, Islamic Azad University, Shiraz 71331, Iran
3.
Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz 71331, Iran
4.
Persian Gulf and Oman Sea Ecological Research Center, Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO), Bandar Abbas 79131, Iran
5.
Iranian National Institute of Oceanography and Atmospheric Science, Tehran 1414, Iran
6.
Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas 79131, Iran

Received Date: 2018-12-17

Abstract

Abstract

Screening bioactive natural products from bacteria is a determinative step in the drug discovery programs. The present study aim to isolate actinobacteria from the Oman Sea sediments for determining the effects of different culture media and treatments on the yield of the isolation process, and measure the DPPH radical scavenging and Artemia cytotoxic activity of culture extracts of the actinobacterial isolates. A total of 290 actinobacterial isolates were collected from 14 sediment samples. Heat treatment (40.68%) and M₄ medium (29.31%) exhibited the maximum isolation rates of actinobacteria. Streptomyces isolates were dominantly distributed in all of the investigated stations according to 16S rRNA gene sequencing. The distribution pattern of Streptomyces followed a depth-dependent frequency trend, whereas the members of rare genera including Micromonospora, Nocardia Actinoplanes, Nocardiopsis, Saccharopolyspora and Crossiella were distributed in deeper stations. Approximately, 25% of the examined isolates could scavenge 90% of 10^-4 mol/L DPPH solutions at 1 250 μg/mL final concentration of their ethyl acetate culture extracts. Furthermore, the most potent extracts could scavenge DPPH radicals with IC₅₀ ranges from 356.8 to 566.4 μg/mL. Brine shrimp cytotoxicity tests showed that 38.88% of the examined culture extracts exhibited LC₅₀ lower than 1 000 μg/mL against the Artemia cells. Moreover, the most potent culture extracts exhibited LC₅₀ range from 335.4 to 534.4 μg/mL. Phylogenetic analysis by 16S rRNA gene sequence revealed that the OS 005, OS 263 and OS 157 closely related to Streptomyces djakartensis, Streptomyces olivaceus and Nocardiopsis dassonvillei respectively. These results suggested the widespread distribution of the antioxidant and cytotoxic producing actinobacteria in the Oman Sea sediments, which could be considered as promising candidates for the discovery of microbial bioactive compounds.
- antioxidant activity,
- cytotoxic activity,
- marine actinobacteria,
- Oman Sea,
- phylogenetic analysis

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