GAO Xiang, DING Guanghui, LI Xishan, XIONG Deqi. Comparison of toxicity effects of fuel oil treated by different dispersants on marine medaka (Oryzias melastigma) embryo[J]. Acta Oceanologica Sinica, 2018, 37(11): 123-132. doi: 10.1007/s13131-018-1255-8
Citation: GAO Xiang, DING Guanghui, LI Xishan, XIONG Deqi. Comparison of toxicity effects of fuel oil treated by different dispersants on marine medaka (Oryzias melastigma) embryo[J]. Acta Oceanologica Sinica, 2018, 37(11): 123-132. doi: 10.1007/s13131-018-1255-8

Comparison of toxicity effects of fuel oil treated by different dispersants on marine medaka (Oryzias melastigma) embryo

doi: 10.1007/s13131-018-1255-8
  • Received Date: 2017-11-21
  • This study aims to evaluate the subacute toxic effects of oil under different treatments on marine organism by simulating natural contaminative processes. In this study, 120# (RMD15) fuel oil was selected as the pollutant and marine medaka (Oryzias melastigma) embryos as the experimental organism. The developmental toxicity of different volume concentrations (0.05%, 0.2%, 1% and 5%) of water-accommodated fractions, biologically-enhanced water-accommodated fractions, and chemically-enhanced water-accommodated fractions on the embryos in different exposure time (8, 15 and 22 d) were compared and the content of relevant polycyclic aromatic hydrocarbons (PAHs) was studied (in dispersion and in vivo). The subacute toxic effects were assessed in terms of antioxidant activities of enzymes (superoxide dismutase, catalase and glutathione S-transferase) and the blue sac disease (BSD) indexes.The results showed that the BSD indexes of the treatment groups were significantly higher than the respective control groups and showed positive correlations with both concentration and exposure time. The experiments with three antioxidant enzymes indicated that enzymatic activities of the embryos changed dramatically under the oxidation stress of petroleum hydrocarbons, especially after adding the dispersants. With the increase of petroleum hydrocarbon concentration and exposure time, the three enzymes showed different degrees of induction and inhibition effects.
  • loading
  • Almeda R, Bona S, Foster C R, et al. 2014. Dispersant Corexit 9500A and chemically dispersed crude oil decreases the growth rates of meroplanktonic barnacle nauplii (Amphibalanus improvisus) and tornaria larvae (Schizocardium sp.). Marine Environmental Research, 99:212-217
    Barron M G, Ka'Aihue L. 2003. Critical evaluation of CROSERF test methods for oil dispersant toxicity testing under subarctic conditions. Marine Pollution Bulletin, 46(9):1191-1199
    Bellas J, Thor P. 2007. Effects of selected PAHs on reproduction and survival of the calanoid copepod Acartia tonsa. Ecotoxicology, 16(6):465-474
    Billiard S M, Hahn M E, Franks D G, et al. 2002. Binding of polycyclic aromatic hydrocarbons (PAHs) to teleost aryl hydrocarbon receptors (AHRs). Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology, 133(1):55-68
    Bo Jun, Cai Ling, Xu Jiahe, et al. 2011. The marine medaka Oryzias melastigma——A potential marine fish model for innate immune study. Marine Pollution Bulletin, 63(5-12):267-276
    Canova S, Degan P, Peters L D, et al. 1998. Tissue dose, DNA adducts, oxidative DNA damage and CYP1A-immunopositive proteins in mussels exposed to waterborne benzo. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 399(1):17-30
    Carls M G, Rice S D, Hose J E. 1999. Sensitivity of fish embryos to weathered crude oil:Part I. Low-level exposure during incubation causes malformations, genetic damage, and mortality in larval pacific herring (Clupea pallasi). Environmental Toxicology and Chemistry, 18(3):481-493
    Chapman H, Purnell K, Law R J, et al. 2007. The use of chemical dispersants to combat oil spills at sea:A review of practice and research needs in Europe. Marine Pollution Bulletin, 54(7):827-838
    Cheung C C C, Siu W H L, Richardson B J, et al. 2004. Antioxidant responses to benzo. Environmental Pollution, 128(3):393-403
    Cohen A M, Nugegoda D. 2000. Toxicity of three oil spill remediation techniques to the Australian bass Macquaria novemaculeata. Ecotoxicology and Environmental Safety, 47(2):178-185
    Couillard C M, Lee K, Légaré B, et al. 2005. Effect of dispersant on the composition of the water-accommodated fraction of crude oil and its toxicity to larval marine fish. Environmental Toxicology and Chemistry, 24(6):1496-1504
    Duarte R M, Honda R T, Val A L. 2010. Acute effects of chemically dispersed crude oil on gill ion regulation, plasma ion levels and haematological parameters in tambaqui (Colossoma macropomum). Aquatic Toxicology, 97(2):134-141
    Dubansky B, Whitehead A, Miller J T, et al. 2013. Multitissue molecular, genomic, and developmental effects of the Deepwater Horizon oil spill on resident Gulf killifish (Fundulus grandis). Environmental Science & Technology, 47(10):5074-5082
    Dussauze M, Danion M, Le Floch S, et al. 2015. Innate immunity and antioxidant systems in different tissues of sea bass (Dicentrarchus labrax) exposed to crude oil dispersed mechanically or chemically with Corexit 9500. Ecotoxicology and Environmental Safety, 120:270-278
    Góth L. 1991. A simple method for determination of serum catalase activity and revision of reference range. Clinica Chimica Acta, 196(2-3):143-151
    González-Doncel M, González L, Fernández-Torija C, et al. 2008. Toxic effects of an oil spill on fish early life stages may not be exclusively associated to PAHs:studies with Prestige oil and medaka (Oryzias latipes). Aquatic Toxicology, 87(4):280-288
    Goodbody-Gringley G, Wetzel D L, Gillon D, et al. 2013. Toxicity of deepwater horizon source oil and the chemical dispersant, corexit® 9500, to coral larvae. Plos One, 8(1):1-10
    Incardona J P, Collier T K, Scholz N L. 2004. Defects in cardiac function precede morphological abnormalities in fish embryos exposed to polycyclic aromatic hydrocarbons. Toxicology and Applied Pharmacology, 196(2):191-205
    Incardona J P, Day H L, Collier T K, et al. 2006. Developmental toxicity of 4-ring polycyclic aromatic hydrocarbons in zebrafish is differentially dependent on AH receptor isoforms and hepatic cytochrome P4501A metabolism. Toxicology and Applied Pharmacology, 217(3):308-321
    Jakoby W B. 1985. Glutathione transferases:an overview. Methods in Enzymology, 113:495-499
    Jung S W, Kwon O Y, Joo C K, et al. 2012. Stronger impact of dispersant plus crude oil on natural plankton assemblages in short-term marine mesocosms. Journal of Hazardous Materials, 217-218:338-349
    Ko J Y, Day J W. 2004. A review of ecological impacts of oil and gas development on coastal ecosystems in the Mississippi Delta. Ocean & Coastal Management, 47(11-12):597-623
    Lee J, Kim M H, Ha M N, et al. 2010. Urinary metabolic profiling of volatile organic compounds in acute exposed volunteers after an oil spill in Republic of Korea. Biomedical Chromatography, 24(5):562-568
    Lee K W, Shim W J, Yim U H, et al. 2013. Acute and chronic toxicity study of the water accommodated fraction (WAF), chemically enhanced WAF (CEWAF) of crude oil and dispersant in the rock pool copepod Tigriopus japonicus. Chemosphere, 92(9):1161-1168
    Livingstone D R, Martinez P G, Michel X, et al. 1990. Oxyradical production as a pollution-mediated mechanism of toxicity in the common mussel, Mytilus edulis L., and other molluscs. Functional Ecology, 4(3):415-424
    Lloyd-Jones G, Lau P C. 1997. Glutathione S-transferase-encoding gene as a potential probe for environmental bacterial isolates capable of degrading polycyclic aromatic hydrocarbons. Applied and Environmental Microbiology, 63(8):3286-3290
    Lloyd-Jones G, Laurie A D, Hunter D W F, et al. 1999. Analysis of catabolic genes for naphthalene and phenanthrene degradation in contaminated New Zealand soils. FEMS Microbiology Ecology, 29(1):69-79
    Lyons M C, Wong D K H, Mulder I, et al. 2011. The influence of water temperature on induced liver EROD activity in Atlantic cod (Gadus morhua) exposed to crude oil and oil dispersants. Ecotoxicology and Environmental Safety, 74(4):904-910
    Marques J A, De Assis H C S, Guiloski I C, et al. 2014. Antioxidant defense responses in Mytella guyanensis (Lamarck, 1819) exposed to an experimental diesel oil spill in Paranaguá Bay (Paraná, Brazil). Ecotoxicology and Environmental Safety, 107:269-275
    Mcintosh S, King T, Wu Dongmei, et al. 2010. Toxicity of dispersed weathered crude oil to early life stages of Atlantic herring (Clupea harengus). Environmental Toxicology and Chemistry, 29(5):1160-1167
    Milinkovitch T, Imbert N, Sanchez W, et al. 2013. Toxicological effects of crude oil and oil dispersant:biomarkers in the heart of the juvenile golden grey mullet (Liza aurata). Ecotoxicology and Environmental Safety, 88:1-8
    Moermond C T A, Zwolsman J J G, Koelmans A A. 2005. Black carbon and ecological factors affect in situ biota to sediment accumulation factors for hydrophobic organic compounds in flood plain lakes. Environmental Science & Technology, 39(9):3101-3109
    Mu Jingli, Wang Xinhong, Jin Fei, et al. 2012. The role of cytochrome P4501A activity inhibition in three-to five-ringed polycyclic aromatic hydrocarbons embryotoxicity of marine medaka (Oryzias melastigma). Marine Pollution Bulletin, 64(7):1445-1451
    Nácher-Mestre J, Serrano R, Benedito-Palos L, et al. 2010. Bioaccumulation of polycyclic aromatic hydrocarbons in gilthead sea bream (Sparus aurata L.) exposed to long term feeding trials with different experimental diets. Archives of Environmental Contamination and Toxicology, 59(1):137-146
    Ōyanagui Y. 1984. Reevaluation of assay methods and establishment of kit for superoxide dismutase activity. Analytical Biochemistry, 142(2):290-296
    Pérez-Cadahía B, Lafuente A, Cabaleiro T, et al. 2007. Initial study on the effects of Prestige oil on human health. Environment International, 33(2):176-185
    Peterson C H, Rice S D, Short J W, et al. 2003. Long-term ecosystem response to the exxon valdez oil spill. Science, 302(5653):2082-2086
    Ramachandran S D, Hodson P V, Khan C W, et al. 2004. Oil dispersant increases PAH uptake by fish exposed to crude oil. Ecotoxicology and Environmental Safety, 59(3):300-308
    Richardson B J, Mak E, De Luca-Abbott S B, et al. 2008. Antioxidant responses to polycyclic aromatic hydrocarbons and organochlorine pesticides in green-lipped mussels (Perna viridis):do mussels "integrate" biomarker responses? Marine Pollution Bulletin, 57(6-12):503-514
    Schein A, Scott J A, Mos L, et al. 2009. Oil dispersion increases the apparent bioavailability and toxicity of diesel to rainbow trout (Oncorhynchus mykiss). Environmental Toxicology and Chemistry, 28(3):595-602
    Scott J. 2009. The mechanism of retene toxicity in the early life stages of fish[dissertation]. Kingston, Ontario, Canada:Queen's University
    Singer M M, Aurand D, Bragin G E, et al. 2000. Standardization of the preparation and quantitation of water-accommodated fractions of petroleum for toxicity testing. Marine Pollution Bulletin, 40(11):1007-1016
    Skarphéðinsdóttir H, Ericson G, Dalla Z L, et al. 2003. Tissue differences, dose-response relationship and persistence of DNA adducts in blue mussels (Mytilus edulis L.) exposed to benzo[a]pyrene. Aquatic Toxicology, 62(2):165-177
    Swannell R P J, Daniel F. 1999. Effect of Dispersants on Oil Biodegradation Under Simulated Marine Conditions. International Oil Spill Conference Proceedings, 1999(1):169-176
    Thorsen W A, Cope W G, Shea D. 2004. Bioavailability of PAHs:effects of soot carbon and PAH source. Environmental Science & Technology, 38(7):2029-2037
    Tissier F, Dussauze M, Lefloch N, et al. 2015. Effect of dispersed crude oil on cardiac function in seabass Dicentrarchus labrax. Chemosphere, 134:192-198
    Wang Zhendi, Fingas M, Li Ken. 1994. Fractionation of a light crude oil and identification and quantitation of aliphatic, aromatic, and biomarker compounds by GC-FID and GC-MS, Part Ⅱ. Journal of Chromatographic Science, 32(9):367-382
    White P A, Robitaille S, Rasmussen J B. 1999. Heritable reproductive effects of benzo. Environmental Toxicology and Chemistry, 18(8):1843-1847
    Wu Dongmei, Wang Zhendi, Hollebone B, et al. 2012. Comparative toxicity of four chemically dispersed and undispersed crude oils to rainbow trout embryos. Environmental Toxicology and Chemistry, 31(4):754-765
    Wu Yuli, Wang Xinhong, Li Yongyu, et al. 2011. Occurrence of polycyclic aromatic hydrocarbons (PAHs) in seawater from the Western Taiwan Strait, China. Marine Pollution Bulletin, 63:459-463
  • 加载中


    通讯作者: 陈斌,
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (654) PDF downloads(375) Cited by()
    Proportional views


    DownLoad:  Full-Size Img  PowerPoint