Phylogenetically diverse, acetaldehyde-degrading bacterial community in the deep sea water of the West Pacific Ocean

GAO Boliang; SHANG Xiexie; LI Li; DI Wenjie; ZENG Runying

doi:10.1007/s13131-018-1235-z

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Article Navigation > Acta Oceanologica Sinica > 2018 > 37(8): 54-64

GAO Boliang, SHANG Xiexie, LI Li, DI Wenjie, ZENG Runying. Phylogenetically diverse, acetaldehyde-degrading bacterial community in the deep sea water of the West Pacific Ocean[J]. Acta Oceanologica Sinica, 2018, 37(8): 54-64. doi: 10.1007/s13131-018-1235-z

Citation:

GAO Boliang, SHANG Xiexie, LI Li, DI Wenjie, ZENG Runying. Phylogenetically diverse, acetaldehyde-degrading bacterial community in the deep sea water of the West Pacific Ocean[J]. Acta Oceanologica Sinica, 2018, 37(8): 54-64. doi: 10.1007/s13131-018-1235-z

Citation:

GAO Boliang, SHANG Xiexie, LI Li, DI Wenjie, ZENG Runying. Phylogenetically diverse, acetaldehyde-degrading bacterial community in the deep sea water of the West Pacific Ocean[J]. Acta Oceanologica Sinica, 2018, 37(8): 54-64. doi: 10.1007/s13131-018-1235-z

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Phylogenetically diverse, acetaldehyde-degrading bacterial community in the deep sea water of the West Pacific Ocean

doi: 10.1007/s13131-018-1235-z

1.
School of Life Sciences, Xiamen University, Xiamen 361102, China
2.
State Key Laboratory Breeding Base of Marine Genetic Resource, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China
3.
State Key Laboratory Breeding Base of Marine Genetic Resource, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China;Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361005, China

Received Date: 2017-12-14
Rev Recd Date: 2018-03-13

Abstract

Abstract

As a major aldehyde pollutant widely existing in industry and our daily life, acetaldehyde is more and more harmful to human health. As characteristic habitat niche, bacteria from deep sea environments are abundant and distinctive in heredity, physiology and ecological functions. Thus, the development of acetaldehyde-degrading bacteria from deep sea provides a new method to harness acetaldehyde pollutant. Firstly, in this study, acetaldehyde-degrading bacteria in the deep sea water of the West Pacific Ocean were enriched in situ and in the laboratory respectively, and then the diversity of uncultured bacteria was studied by using 16S rRNA genes. Then acetaldehyde-degrading strains were isolated from two samples, including enrichment in situ and enrichment in laboratory samples of deep sea water from the West Pacific Ocean using acetaldehyde as the sole carbon source, and then the ability of acetaldehyde degradation was detected. Our results showed that the main uncultured bacteria of two samples with different enrichment approaches were similar, including Proteobacteria, Actinobacteria, Firmicutes, Cyanobacteria, but the structure of bacterial community were significant different. Four subgroups, α, γ, δ and ε, were found in Proteobacteria group. The γ-Proteobacteria was dominant (63.5% clones in laboratory enriched sample, 75% clones in situ enriched sample). The species belonged to γ-Proteobacteria and their proportion was nearly identical between the two enrichment samples, and Vibrio was the predominant genus (45% in laboratory enriched sample, 48.5% in situ enriched sample), followed by Halomonas (9% in situ enriched sample) and Streptococcus (6% in laboratory enriched sample). A total of 12 acetaldehyde-degrading strains were isolated from the two samples, which belonged to Vibrio, Halomonas, Pseudoalteromonas, Pseudomonas and Bacillus of γ-Proteobacteria. Strains ACH-L-5, ACH-L-8 and ACH-S-12, belonging to Vibrio and Halomonas, have strong ability of acetaldehyde degradation, which could tolerate 1.5 g/L acetaldehyde and degrade 350 mg/L acetaldehyde within 24 hours. Our results indicated that bacteria of γ-Proteobacteria may play an important role in carbon cycle of deep sea environments, especial the bacteria belonging to Vibrio and Halomonas and these strains was suggested for their potentials in government of aldehyde pollutants.
- acetaldehyde-degrading bacteria,
- ALDH,
- deep sea,
- in situ

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References(41)

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