ZHANG Shoukang, ZHENG Jiao, ZHANG Jing, WANG Zhiyong, WANG Yilei, CAI Mingyi. Cytogenetic characterization and description of an X1X1X2X2/X1X2Y sex chromosome system in Collichthys lucidus (Richardson, 1844)[J]. Acta Oceanologica Sinica, 2018, 37(4): 34-39. doi: 10.1007/s13131-018-1152-1
Citation: ZHANG Shoukang, ZHENG Jiao, ZHANG Jing, WANG Zhiyong, WANG Yilei, CAI Mingyi. Cytogenetic characterization and description of an X1X1X2X2/X1X2Y sex chromosome system in Collichthys lucidus (Richardson, 1844)[J]. Acta Oceanologica Sinica, 2018, 37(4): 34-39. doi: 10.1007/s13131-018-1152-1

Cytogenetic characterization and description of an X1X1X2X2/X1X2Y sex chromosome system in Collichthys lucidus (Richardson, 1844)

doi: 10.1007/s13131-018-1152-1
  • Received Date: 2017-03-02
  • The chromosomes of spinyhead croaker Collichthys lucidus (Richardson, 1844) were characterized for the first time by fluorescence staining, self genomic in situ hybridization (self-GISH), and multicolor fluorescence in situ hybridization (FISH) with 18S rDNA, 5S rDNA and telomeric sequence probes. The female karyotype has exclusively 24 pairs of acrocentric chromosomes (2n=48a, NF=48), while the male one consists of 22 pairs of acrocentric chromosomes, 2 monosomic acrocentric chromosomes and a metacentric chromosome (2n=1m+46a, NF=48). The difference between female and male karyotypes indicates the presence of a sex chromosome of X1X1X2X2/X1X2Y type, where Y is the unique metacentric chromosome in the male karyotype. As revealed by FISH, 5S rDNA and 18S rDNA sites were mapped at syntenic position of the largest acrocentric chromosome (X1), and the short arms of the Y chromosome as well. An X1-chromosome specific interstitial telomeric signal (ITS) was detected overlapping the 5S rDNA sites. In addition, self-GISH revealed that the repetitive DNAs accumulated on all the putative sex chromosome. Chromosome fusion accompanied by a partial deletion in the ancestral karyotype (2n=48a) is hypothesized for the origin of such multiple sex chromosome system. The present study, as the first description of differentiated sex chromosome in family Sciaenidae, will give clues to the studies on the sex chromosome of other Sciaenids.
  • loading
  • Accioly I V, Molina W F. 2008. Cytogenetic studies in Brazilian marine Sciaenidae and Sparidae fishes (Perciformes). Genet Mol Res, 7(2): 358-370
    Arai R. 2011. Fish Karyotypes: A Check List. Tokyo: Springer, 163–209
    Artoni R F, Bertollo L A C. 2002. Evolutionary aspects of the ZZ/ZW sex chromosome system in the Characidae fish, genus Triportheus. A monophyletic state and NOR location on the W chromosome. Heredity, 89(1): 15-19
    Bitencourt J A, Sampaio I, Ramos R T C, et al. 2017. First report of sex chromosomes in Achiridae (Teleostei: Pleuronectiformes) with inferences about the origin of the multiple X1X1X2X2/X1X2Y system and dispersal of ribosomal genes in Achirus achirus. Zebrafish, 14(1): 90-95
    Blanco D R, Vicari M R, Lui R L, et al. 2014. Origin of the X1X1X2X2/X1X2Y sex chromosome system of Harttia punctata (Siluriformes, Loricariidae) inferred from chromosome painting and FISH with ribosomal DNA markers. Genetica, 142(2): 119-126
    Born G G, Bertollo L A C. 2000. An XX/XY sex chromosome system in a fish species, Hoplias malabaricus, with a polymorphic NOR-bearing X chromosome. Chromosome Res, 8(2): 111-118
    Chalopin D, Volff J N, Galiana D, et al. 2015. Transposable elements and early evolution of sex chromosomes in fish. Chromosome Res, 23(3): 545-560
    Charlesworth D, Charlesworth B, Marais G. 2005. Steps in the evolution of heteromorphic sex chromosomes. Heredity, 95(2): 118-128
    Chen Songlin, Zhang Guojie, Shao Changwei, et al. 2014. Whole-genome sequence of a flatfish provides insights into ZW sex chromosome evolution and adaptation to a benthic lifestyle. Nat Genet, 46(3): 253-260
    Cheng Jiao, Ma Guoqiang, Miao Zhenqing, et al. 2012. Complete mitochondrial genome sequence of the spinyhead croaker Collichthys lucidus (Perciformes, Sciaenidae) with phylogenetic considerations. Mol Biol Rep, 39(4): 4249-4259
    Devlin R H, Nagahama Y. 2002. Sex determination and sex differentiation in fish: an overview of genetic, physiological, and environmental influences. Aquaculture, 208(3-4): 191-364
    Diniz D, Moreira-Filho O, Bertollo L A C. 2008. Molecular cytogenetics and characterization of a ZZ/ZW sex chromosome system in Triportheus nematurus (Characiformes, Characidae). Genetica, 133(1): 85-91
    Ferreira M, Garcia C, Matoso D A, et al. 2016. A new multiple sex chromosome system X1X1X2X2/X1Y1X2Y2 in Siluriformes: cytogenetic characterization of Bunocephalus coracoideus (Aspredinidae). Genetica, 144(5): 591-599
    Gold J R, Li Y C, Shipley N S, et al. 1990. Improved methods for working with fish chromosomes with a review of metaphase chromosome banding. J Fish Biol, 37(4): 563-575
    Gornung E. 2013. Twenty years of physical mapping of major ribosomal RNA genes across the teleosts: a review of research. Cytogenet Genome Res, 141(2-3): 90-102
    Graves J A M. 2006. Sex chromosome specialization and degeneration in mammals. Cell, 124(5): 901-914
    Howell W M, Black D A. 1979. Location of the nucleolus organizer regions on the sex chromosomes of the banded killifish, Fundulus diaphanus. Copeia, 1979(3): 544-546
    Ijdo J W, Wells R A, Baldini A, et al. 1991. Improved telomere detection using a telomere repeat probe (TTAGGG)n generated by PCR. Nucleic Acids Res, 19(17): 4780
    Kitano J, Peichel C L. 2012. Turnover of sex chromosomes and speciation in fishes. Environ Biol Fishes, 94(3): 549-558
    Kitano J, Ross J A, Mori S, et al. 2009. A role for a neo-sex chromosome in stickleback speciation. Nature, 461(7267): 1079-1083
    Levan A, Fredga K, Sandberg A A. 1964. Nomenclature for centromeric position on chromosomes. Hereditas, 52(2): 201-220
    Liao Mengxian, Zheng Jiao, Wang Zhiyong, et al. 2017. Molecular cytogenetic of the Amoy croaker, Argyrosomus amoyensis (Teleostei, Sciaenidae). Chin J Oceanol Limnol, doi: 10.1007/s00343-018-6272-0
    Liu Zhiyong, Moore P H, Ma Hao, et al. 2004. A primitive Y chromosome in papaya marks incipient sex chromosome evolution. Nature, 427(6972): 348-352
    Nelson J S, Grande T C, Wilson M V H. 2016. Fishes of the World. 5th ed. New York: John Wiley and Sons Inc, 498–499
    Palacios-Gimenez O M, Castillo E R, Martí D A, et al. 2013. Tracking the evolution of sex chromosome systems in Melanoplinae grasshoppers through chromosomal mapping of repetitive DNA sequences. BMC Evol Biol, 13: 167
    Poltronieri J, Marquioni V, Bertollo L A C, et al. 2013. Comparative chromosomal mapping of microsatellites in Leporinus species (Characiformes, Anostomidae): unequal accumulation on the W chromosomes. Cytogenet Genome Res, 142(1): 40-45
    Reed K M, Phillips R B. 1995. Molecular cytogenetic analysis of the double-CMA3 chromosome of lake trout, Salvelinus namaycush. Cytogenet Cell Genet, 70(1-2): 104-107
    Reed K M, Phillips R B. 1997. Polymorphism of the nucleolus organizer region (NOR) on the putative sex chromosomes of Arctic char (Salvelinus alpinus) is not sex related. Chromosome Res, 5(4): 221-227
    Ren X J, Eisenhour L, Hong C S, et al. 1997. Roles of rDNA spacer and transcription unit-sequences in X-Y meiotic chromosome pairing in Drosophila melanogaster males. Chromosoma, 106(1): 29-36
    Ross J A, Peichel C L. 2008. Molecular cytogenetic evidence of rearrangements on the Y chromosome of the threespine stickleback fish. Genetics, 179(4): 2173-2182
    Ross J A, Urton J R, Boland J, et al. 2009. Turnover of sex chromosomes in the stickleback fishes (Gasterosteidae). PLoS Genet, 5(2): e1000391
    She Chaowen, Liu Jingyu, Diao Ying, et al. 2007. The distribution of repetitive DNAs along chromosomes in plants revealed by self-genomic in situ hybridization. J Genet Genomics, 34(5): 437-448
    Stitou S, Burgos M, Zurita F, et al. 1997. Recent evolution of NOR-bearing and sex chromosomes of the North African rodent Lemniscomys barbarus. Chromosome Res, 5(7): 481-485
    Uyeno T, Miller R R. 1971. Multiple sex chromosomes in a Mexican cyprinodontid fish. Nature, 231(5303): 452-453
    Yano C F, Poltronieri J, Bertollo L A C, et al. 2014. Chromosomal mapping of repetitive DNAs in Triportheus trifurcatus (Characidae, Characiformes): insights into the differentiation of the Z and W chromosomes. PLoS One, 9(3): e90946
    Zheng Jiao, Cao Kuan, Yang Anran, et al. 2016. Chromosome mapping using genomic DNA and repetitive DNA sequences as probes for somatic chromosome identification in Nibea albiflora. J Fish China (in Chinese), 40(8): 1156-1162
  • 加载中


    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (803) PDF downloads(774) Cited by()
    Proportional views


    DownLoad:  Full-Size Img  PowerPoint