Long duplication of 18S ribosomal DNA in <i>Cynoglossus lineolatus</i> (Pleuronectiformes: Cynoglossidae): novel molecular evidence for unequal crossing over model

GONG Li; SHI Wei; YANG Min; SI Lizhen; KONG Xiaoyu

doi:10.1007/s13131-016-0957-z

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Article Navigation > Acta Oceanologica Sinica > 2016 > 35(12): 38-50

GONG Li, SHI Wei, YANG Min, SI Lizhen, KONG Xiaoyu. Long duplication of 18S ribosomal DNA in Cynoglossus lineolatus (Pleuronectiformes: Cynoglossidae): novel molecular evidence for unequal crossing over model[J]. Acta Oceanologica Sinica, 2016, 35(12): 38-50. doi: 10.1007/s13131-016-0957-z

Citation:

GONG Li, SHI Wei, YANG Min, SI Lizhen, KONG Xiaoyu. Long duplication of 18S ribosomal DNA in Cynoglossus lineolatus (Pleuronectiformes: Cynoglossidae): novel molecular evidence for unequal crossing over model[J]. Acta Oceanologica Sinica, 2016, 35(12): 38-50. doi: 10.1007/s13131-016-0957-z

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Long duplication of 18S ribosomal DNA in Cynoglossus lineolatus (Pleuronectiformes: Cynoglossidae): novel molecular evidence for unequal crossing over model

doi: 10.1007/s13131-016-0957-z

1.
Guangxi Key Laboratory of Mangrove Conservation and Utilization, Guangxi Mangrove Research Center, Guangxi Academy of Sciences, Beihai 536000, China;Key Laboratory of Tropical Marine Bio-resources and Ecology/Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510000, China;University of the Chinese Academy of Sciences, Beijing 100049, China
2.
Key Laboratory of Tropical Marine Bio-resources and Ecology/Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510000, China
3.
Key Laboratory of Tropical Marine Bio-resources and Ecology/Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510000, China;University of the Chinese Academy of Sciences, Beijing 100049, China

Received Date: 2015-10-18
Rev Recd Date: 2016-05-13

Abstract

Abstract

Although 18S rDNA sequence is extremely conservative, the polymorphism still has been found in few species. In the present study, three types (Type A, B and C) of 18S rDNA sequence coexisted in Cynoglossus lineolatus genome, suggesting a non-concerted evolution process, rather than a strictly concerted evolution fashion. Based on the differences of sequence variation, GC content, secondary structure and minimum free energy, Types A and B were speculated as the potential pseudogenes. Additionally, a fascinating finding was a 189-bp duplication of 18S rDNA in Type A sequence. To our knowledge, this is the first report on such a long duplication in teleostean ribosomal DNA. Compared with several theories accounting for the formation of tandem repeats, the unequal crossing over model was thought to be the most likely mechanism to generate the 189-bp duplication of 18S rDNA. These results not only provide a novel molecular evidence for the unequal crossing over model, but also benefit for the further study on 18S rDNA in fishes.
- nrDNA,
- Cynoglossus lineolatus,
- tandem repeat,
- pseudogene,
- polymorphism,
- non-concerted evolution

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References(37)

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