ANDRIANTAHINA Farafidy, LIU Xiaolin, HUANG Hao. Using microsatellite markers to identify heritability of Pacific whiteleg shrimp Litopenaeus vannamei[J]. Acta Oceanologica Sinica, 2015, 34(6): 59-65. doi: 10.1007/s13131-015-0688-6
Citation: ANDRIANTAHINA Farafidy, LIU Xiaolin, HUANG Hao. Using microsatellite markers to identify heritability of Pacific whiteleg shrimp Litopenaeus vannamei[J]. Acta Oceanologica Sinica, 2015, 34(6): 59-65. doi: 10.1007/s13131-015-0688-6

Using microsatellite markers to identify heritability of Pacific whiteleg shrimp Litopenaeus vannamei

doi: 10.1007/s13131-015-0688-6
  • Received Date: 2014-01-15
  • Rev Recd Date: 2014-05-16
  • Pacific whiteleg shrimp (Litopenaeus vannamei) is an economically relevant shrimp species in many Asian countries. The specific objective of the current research was to assess microsatellite markers in screening the fastgrowth of domesticated L. vannamei stocks to establish a founder population for breeding-selection plans. The postlarvae produced by the reproduction of second generation broodstock were cultured in the same conditions throughout a five months growing period. Ninety juvenile shrimp were selected from the slow-, medium- and the fast-growth groups, and ten microsatellite markers were used to investigate their genetic diversity, and to understand the improvement of a breeding-selection scheme. Ten polymorphic loci (markers) (M1-M10) were produced at ten loci in this sample, among them Primer M8 was the highest polymorphic locus and M7 was the lowest one. A specific locus was found in the fast-growth group using Primer M5. The longest genetic distance (0.481) was determined between the fast- and medium-growth groups and the shortest (0.098) was between the slow- and medium-growth groups; therefore, the largest genetic identity (0.946) was observed between the slowand medium-growth groups and the smallest (0.667) was observed between the medium- and fast-growth groups. The Unweighted Paired Group with Arithmetic Average (UPGMA) dendrogram based on Nei's genetic distances provided two different groups; the first consist of the slow- and medium-growth groups and the second the fastgrowth group. Selection response and realized heritability for growth were 11.55% and 31.26%, respectively. Therefore, this set of microsatellite markers would provide a useful tool in shrimp breeding schemes.
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  • Andriantahina F, Liu Xiaolin, Feng Tingting, et al. 2013a. Current status of genetics and genomics of reared penaeid shrimp: in-formation relevant to access and benefit sharing. Marine Bio-technology, 15(4): 399-412
    Andriantahina F, Liu Xiaolin, Huang Hao, et al. 2012. Comparison of reproductive performance and offspring quality of domestic-ated Pacific white shrimp, Litopenaeus vannamei. Aquaculture, 324-325: 194-200
    Andriantahina F, Liu Xiaolin, Huang Hao, et al. 2013b. Selection for growth performance of tank-reared Pacific white shrimp, Litopenaeus vannamei. Chinese Journal of Oceanology Limno-logy, 31(3): 534-541
    Arce S M, Moss S M, Argue B J. 2000. Artificial insemination and spawning of Pacific white shrimp, Litopenaeus vannamei: im-plications for a selective breeding program. In: Tamaru C C T, Tamaru C S, McVey J P, et al., eds. Spawning and Maturation of Aquatic Species: Proceedings of the Twenty-Eighth US-Japan Natural Resources Aquaculture Panel. Hawaii: University of Hawaii Sea Grant College Program. UJNR Technical Report, 28: 5-8
    Argue J B, Arce S M, Lotz M J, et al. 2002. Selective breeding of pacific white shrimp (Litopenaeus vannamei) for growth and resist-ance to Taura syndrome virus. Aquaculture, 204(3-4): 447-460
    Benzie J A H. 2009. Use and exchange of genetic resources of penaeid shrimps for food and aquaculture. Reviews in Aquaculture, 1(3-4): 232-250
    Benzie J A H, Kenway M, Trott L. 1997. Estimates for the heritability of size in juvenile Penaeus monodon prawns from half-sib mat-ings. Aquaculture, 152(1-4): 49-53
    Bhassu S, See L M, Hassan R, et al. 2008. Isolation and characteriza-tion of microsatellite loci in the Malaysian giant freshwater prawn, Macrobrachium rosenbergii. Molecular Ecology Re-sources, 8(5): 983-985
    Bierne N, Beuzart I, Vonau V, et al. 2000. Microsatellite-associated heterosis in hatchery-propagated stocks of the shrimp Penaeus stylirostris. Aquaculture, 184(3-4): 203-219
    Bruni I. 2010. Il genere Rhododendron L.: diversità genetica e fenomeni di ibridazione (in Italian) [dissertation]. Bologna: Alma Mater Studiorum—Università di Bologna, 130
    Chen Limei, Li Qi, Yang Jianmin. 2008. Microsatellite genetic vari-ation in wild and hatchery populations of the sea cucumber (Apostichopus japonicus Selenka) from northern China. Aquaculture Research, 39(14): 1541-1549
    Cock J, Gitterle T, Salazar M, et al. 2009. Breeding for disease resist-ance of Penaeid shrimps. Aquaculture, 286(1-2): 1-11
    Coman G J, Arnold S J, Wood A T, et al. 2010. Age: age genetic correla-tions for weight of Penaeus monodon reared in broodstock tank systems. Aquaculture, 307(1-2): 1-5
    Cruz P, Ibarra A M, Mejia-Ruiz H, et al. 2004. Genetic variability as-sessed by microsatellite in a breeding program of Pacific White Shrimp (Litopenaeus vannamei). Marine Biotechnology, 6(2): 157-164
    De Donato M, Manrique R, Ramirez R, et al. 2005. Mass selection and inbreeding effect on a cultivated strain of Penaeus (Litopen-aeus) vannamei in Venezuela. Aquaculture, 247(1-4): 159-167
    De la Rosa-Vélez J, Escobar R, Correa F, et al. 1999. High allozyme variation and genetic similarity of two populations of commer-cial penaeids, Penaeus brevirostris (Kingsley) and P. vannamei (Boone), from the Gulf of California. Aquaculture Research, 30(6): 459-463
    Divu D, Karunasagar I, Karunasagar I. 2008. Microsatellite DNA markers in the giant freshwater prawn, Macrobrachium rosen-bergii: a tool for genetic analysis. Molecular Ecology Resources, 8(5): 1040-1042
    Dong Shirui, Kong Jie, Zhang Tianshi, et al. 2006. Parentage determ-ination of Chinese shrimp (Fenneropenaeus chinensis) based on microsatellite DNA markers. Aquaculture, 258(1-4): 283-288
    Falconer D S, Macka T F C. 1996. Introduction to Quantitative Genet-ics. New York: Longman, 438
    Farafidy A, Liu Xiaolin, Huang Hao, et al. 2012. Response to selection, heritability and genetic correlations between body weight and body size in Pacific white shrimp, Litopenaeus vannamei. Chinese Journal of Oceanology and Limnology, 30(2): 200-205
    Fofana I J, Ofori D, Poitel M, et al. 2009. Diversity and genetic struc-ture of teak (Tectona grandis L.f) in its natural range using DNA microsatellite markers. New Forests, 37(2): 175-195
    Garcia D K, Benzie J A H. 1995. RAPD markers of potential use in pen-aeid prawn (Penaeus monodon) breeding programs. Aquacul-ture, 130(2-3): 137-144
    Gitterle T, Rye M, Salte R, et al. 2005. Genetic (co)variation in harvest body weight and survival in Penaeus (Litopenaeus) vannamei under standard commercial conditions. Aquaculture, 243(1-4): 83-92
    Gjedrem T, Fimland E. 1995. Potential benefits from high health and genetically improved shrimp stocks. In: Browdy C L, Hopkins J S, eds. Swimming Through Water. Proceedings of Special Ses-sion on Shrimp Farming, 95: 235
    Goyard E, Goarant C, Ansquer D, et al. 2008. Cross breeding of differ-ent domesticated lines as a simple way for genetic improve-ment in small aquaculture industries: Heterosis and inbreed-ing effects on growth and survival rates of the Pacific blue shrimp Penaeus (Litopenaeus) stylirostris. Aquaculture, 278(1-4): 43-50
    Goyard E, Patrois J, Peignon J M, et al. 2002. Selection for better growth of Penaeus stylirostris in Tahiti and New Caledonia. Aquaculture, 204(3-4): 461-468
    Hetzel D J S, Crocos P J, Davis G P, et al. 2000. Response to selection and heritability for growth in the Kuruma prawn, Penaeus ja-ponicus. Aquaculture, 181(3-4): 215-223
    Hoa N D. 2009. Domestication of black tiger shrimp (Penaeus monodon) in recirculation systems in Vietnam [dissertation]. Ghent, Belgium: Ghent University, 179
    Jerry D R, Preston N P, Crocos P J, et al. 2004. Parentage determina-tion of Kuruma shrimp Penaeus (Marsupenaeus) japonicus us-ing microsatellite markers (Bate). Aquaculture, 235(1-4): 237-247
    Jung H, Lyons R E, Dinh H, et al. 2011. Transcriptomics of a giant freshwater prawn (Macrobrachium rosenbergii): de novo as-sembly, annotation and marker discovery. PLoS ONE, 6(12): e27938
    Karaket T, Poompuang S, Na-Nakorn U, et al. 2011. DNA microsatel-lite-based evaluation of early growth performance among strains of freshwater prawn Macrobrachium rosenbergii de Man. Aquaculture, 311(1-4): 115-122
    Kelly M W, Rhymer J M. 2005. Population genetic structure of a rare unionid (Lampsilis cariosa) in a recently glaciated landscape. Conservation Genetics, 6(5): 789-802
    Keys S J, Crocos P J, Burridge C Y, et al. 2004. Comparative growth and survival of inbred and outbred Penaeus (marsupenaeus) ja-ponicus, reared under controlled environment conditions: in-dications of inbreeding depression. Aquaculture, 241(1-4): 151-168
    Khan S R, Akter H, Sultana N, et al. 2014. Genetic diversity in three river populations of the giant freshwater prawn (Macrobrachi-um rosenbergii) in Bangladesh assessed by microsatellite DNA markers. International Journal of Agriculture & Biology, 16(1): 195-200
    Kheirabadi K, Alijani S, Zavadilová L, et al. 2013. Estimation of genet-ic parameters for daily milk yields of primiparous Iranian Hol-stein cows. Archiv Tierzucht, 56(44): 455-466
    Krishna G, Gopikrishna G, Gopal C, et al. 2011. Genetic parameters for growth and survival in Penaeus monodon cultured in India. Aquaculture, 318 (1-2): 74-78
    Liu Z J, Cordes J F. 2004. DNA marker technologies and their applica-tions in aquaculture genetics. Aquaculture, 238(1-4): 1-37
    Liu Feng, Xia Junhong, Bai Zhiyi, et al. 2009. High genetic diversity and substantial population differentiation in grass carp (Cteno-pharyngodon idella) revealed by microsatellite analysis. Aquaculture, 297(1-4): 51-56
    Ma K Y, Feng J B, Li J L. 2012a. Genetic variation based on microsatel-lite analysis of the oriental river prawn, Macrobrachium nip-ponense from Qiandao Lake in China. Genetics and Molecular Research, 11(4): 4235-4244
    Ma Keyi, Qiu Gaofeng, Feng Jianbin, et al. 2012b. Transcriptome ana-lysis of the oriental river prawn, Macrobrachium nipponense using 454 pyrosequencing for discovery of genes and markers. PLoS One, 7(6): e39727
    Moore S S, Whan V, Davis G P, et al. 1999. The development and ap-plication of genetic markers for the Kuruma prawn Penaeus ja-ponicus. Aquaculture, 173(1-4): 19-32
    Moss D R, Arce S M, Otoshi C A, et al. 2007. Effects of inbreeding on survival and growth of pacific white shrimp Penaeus (Litopen-arus) vannamei. Aquaculture, 272(S1): S30-S37
    Nei M. 1972. Genetic distance between populations. The American Naturalist, 106(949): 283-292
    Nei M, Chakraborty R, Fuerst P A. 1976. Infinite allele model with varying mutation rate. Proceeding of the National Academy of Sciences of the United States of America, 73(11): 4164-4168
    Nelson K, Hedgecock D. 1980. Enzyme polymorphism and adaptive strategy in the decapod Crustacea. The American Naturalist, 116(2): 238-280
    Pourkazemi M, Nazari S, Bakhshalizadeh S. 2010. Karyotype analysis in white bream (Blicca bjoerkna transcaucasica) from north coast of Iran. Iranian Journal of Fisheries Sciences, 9(3): 454-463
    Rezvani Gilkolaei S, Safari R, Laloei F, et al. 2011. Using RAPD mark-ers potential to identify heritability for growth in Fenneropen-aeus indicus. Iranian Journal of Fisheries Sciences, 10(1): 123-134
    Rousset F. 2008. Genepop’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Molecular Ecology Resources, 8(1): 103-106
    Sambrook J, Russell D W. 2001. Molecular Cloning: A Laboratory Manual. 3rd ed. New York: Cold Spring Harbor Laboratory Press, 132-144
    Schneider K J, Tidwell J H, Gomelsky B, et al. 2012. Genetic diversity of cultured and wild populations of the giant freshwater prawn Macrobrachium rosenbergii (de Man, 1879) based on mi-crosatellite analysis. Aquaculture Research, 44(9): 1425-1437
    Sriphairoj K, Kamonrat W, Na-korn U. 2007. Genetic aspect in brood-stock management of the critically endangered Makong giant catfish, Pangasianodon gigas in Thailand. Aquaculture, 264(1-4): 34-46
    Szeker K. 2012. Nucleoside phosphorylases from thermophiles—Re-combinant expression and biocatalytic use for modified nucle-osides [dissertation]. Berlin: Technischen Universit.t, 152
    Tamura K, Dudley J, Nei M, et al. 2007. MEGA4: Molecular and Evolu-tionary Genetics Analysis (MEGA) software version 4.0. Mo-lecular Biology and Evolution, 24(8): 1596-1599
    Taris N, Batista F M, Boudry P. 2007. Evidence of response to unin-tentional selection for faster development and inbreeding de-pression in Crassostrea gigas larvae. Aquaculture, 272(S1): S69-S79
    Van Oosterhout C, Hutchinson W F, Wills D P M, et al. 2004. Micro-Checker: software for identifying and correcting genotyping er-rors in microsatellite data. Molecular Ecology Notes, 4(3): 535-538
    Vaseeharan B, Rajakamaran P, Jayaseelan D, et al. 2013. Molecular markers and their application in genetic diversity of penaeid shrimp. Aquaculture International, 21(2): 219-241
    Wang Le, Meng Zining, Liu Xiaochun, et al. 2011. Genetic diversity and differentiation of the orange-spotted grouper (Epinephelus coioides) between and within cultured stocks and wild popula-tions inferred from microsatellite DNA analysis. International Journal of Molecular Sciences, 12(7): 4378-4394
    Wolfus G M, Garcia D K, Alcivar-Warren A. 1997. Application of the microsatellite technique for analyzing genetic diversity in shrimp breeding programs. Aquaculture, 152(1-4): 35-47
    Wuthisuthimethavee S, Lumubol P, Vanavichit A, et al. 2003. Devel-opment of microsatellite markers in black tiger shrimp (Pen-aeus monodon Fabricius). Aquaculture, 224(1-4): 39-50
    Xu Zhenkang, Primavera J H, de La Pena L D, et al. 2001. Genetic di-versity of wild and cultured black tiger shrimp (Penaeus monodon) in the Philippines using microsatellites. Aquacul-ture, 199(1-2): 13-40
    You E-M, Chiu T-S, Liu K-F, et al. 2008. Microsatellite and mitochon-drial haplotype diversity reveals population differentiation in the tiger shrimp (Penaeus monodon) in the Indo-Pacific region. Animal Genetics, 39(3): 267-277
    Yue Genhua, Zhu Zeyuan, Lo L C, et al. 2009. Genetic variation and population structure of Asian seabass (Lates calcarifer) in the Asia-Pacific region. Aquaculture, 293(1-2): 22-28
    Zima Jr J, Leština D, Konvička M. 2013. Characterization of ten poly-morphic microsatellite markers for an endangered butterfly Ar-gynnis niobe and their cross-species utility in the closely re-lated species A. adippe (Lepidoptera: Nymphalidae). European Journal of Entomology, 110(2): 383-387
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