Phosphorus deficiency affects multiple macromolecular biosynthesis pathways of <i>Thalassiosira weissflogii</i>

WANG Xiuxiu; HUANG Bangqin; ZHANG Huan

doi:10.1007/s13131-014-0413-x

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Article Navigation > Acta Oceanologica Sinica > 2014 > 33(4): 85-91

WANG Xiuxiu, HUANG Bangqin, ZHANG Huan. Phosphorus deficiency affects multiple macromolecular biosynthesis pathways of Thalassiosira weissflogii[J]. Acta Oceanologica Sinica, 2014, 33(4): 85-91. doi: 10.1007/s13131-014-0413-x

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WANG Xiuxiu, HUANG Bangqin, ZHANG Huan. Phosphorus deficiency affects multiple macromolecular biosynthesis pathways of Thalassiosira weissflogii[J]. Acta Oceanologica Sinica, 2014, 33(4): 85-91. doi: 10.1007/s13131-014-0413-x

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Phosphorus deficiency affects multiple macromolecular biosynthesis pathways of Thalassiosira weissflogii

doi: 10.1007/s13131-014-0413-x

1.
Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, Xiamen University, Xiamen 361005, China;State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China
2.
Department of Marine Sciences, University of Connecticut, Groton 06340, USA

Received Date: 2012-09-23
Rev Recd Date: 2012-12-31

Abstract

Abstract

Phosphorus (P) is one of the key nutrients for the growth of phytoplankton. In this study, we used a method coupling label-free quantitation with liquid chromatography-mass spectrometry (LFQ-LC-MS/MS) to track the change of relative protein abundance between P-replete and P-deficient treatments in a non-model diatom, Thalassiosira weissflogii. Out of the 631 proteins identified, 132 were found to have significant changes in abundance (>1.5 folds) between the two treatments, especially those proteins involved in macromolecular biosynthesis pathways. For example, the up-regulation of sulfolipid biosynthesis protein in the P-deficient culture suggested a switch from using phospholipids to sulfolipids. In addition, the ribosome subunits and tRNA synthetases were down-regulated, which might explain the decrease in protein content in the P-deficient culture. A vacuolar sorting receptor homologous protein was found to be 9.2-folds up-regulated under P-deficiency, indicating an enhancement in the vacuolar sorting pathway for protein degradation. Our results show that T. weissflogii has sophisticated responses in multiple macromolecular metabolism pathways under P-deficiency, a mechanism which can be critical for this species to survive under various levels of P availability in the environment.
- phosphorus deficiency,
- Thalassiosira weissflogii,
- LFQ-LC-MS/MS,
- sulfolipid biosynthesis protein

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References(35)

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