YAN Zhenguang, MENG Wei, LIU Zhengtao, YANG Suwen, LIU Xiaojun, SUN Juan, XIE Liping, ZHANG Rongqing. In vivo and in vitro biomineralization in the presence of the inner-shell film of pearl oyster[J]. Acta Oceanologica Sinica, 2011, (1): 87-93. doi: 10.1007/s13131-011-0094-7
Citation:
YAN Zhenguang, MENG Wei, LIU Zhengtao, YANG Suwen, LIU Xiaojun, SUN Juan, XIE Liping, ZHANG Rongqing. In vivo and in vitro biomineralization in the presence of the inner-shell film of pearl oyster[J]. Acta Oceanologica Sinica, 2011, (1): 87-93. doi: 10.1007/s13131-011-0094-7
YAN Zhenguang, MENG Wei, LIU Zhengtao, YANG Suwen, LIU Xiaojun, SUN Juan, XIE Liping, ZHANG Rongqing. In vivo and in vitro biomineralization in the presence of the inner-shell film of pearl oyster[J]. Acta Oceanologica Sinica, 2011, (1): 87-93. doi: 10.1007/s13131-011-0094-7
Citation:
YAN Zhenguang, MENG Wei, LIU Zhengtao, YANG Suwen, LIU Xiaojun, SUN Juan, XIE Liping, ZHANG Rongqing. In vivo and in vitro biomineralization in the presence of the inner-shell film of pearl oyster[J]. Acta Oceanologica Sinica, 2011, (1): 87-93. doi: 10.1007/s13131-011-0094-7
Institute of Marine Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China;Chinese Research Academy of Environmental Sciences, Beijing 100012, China
2.
Chinese Research Academy of Environmental Sciences, Beijing 100012, China
3.
Institute of Marine Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China;Protein Science Laboratory of the Ministry of Education, Tsinghua University, Beijing 100084, China
The inner shell surface is the biomineralization site in shell formation and an inner-shell film covers it. This surface is composed of two regions:an outer calcitic region and an inner aragonitic region. In this study, some amalgamated calcite crystals were found in the calcitic region and some aragonitic "imprints" were found in the central part of the aragonitic region. The "imprints" are probably the trace of mantle cells that adhered to the inner shell surface when the shell was produced. Furthermore, to build a novel in vitro biomineralization system, the inner-shell film was detached from the shell and introduced to the calcitic crystallization solution. Crystallization experiments showed that nacre proteins could induce aragonite crystals in the novel system but inhibited calcite growth in the absence of the inner-shell film. These data suggested that the inner-shell film may induce aragonite growth in vivo by combining nacre proteins.
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