Effects of main ecological factors on the growth of marine green alga Caulerpa sertularioides using the response surface methodology

Bingxin Huang Yue Chu Rongjuan Wang Yixiao Wang Lanping Ding

Bingxin Huang, Yue Chu, Rongjuan Wang, Yixiao Wang, Lanping Ding. Effects of main ecological factors on the growth of marine green alga Caulerpa sertularioides using the response surface methodology[J]. Acta Oceanologica Sinica, 2023, 42(11): 90-97. doi: 10.1007/s13131-023-2171-0
Citation: Bingxin Huang, Yue Chu, Rongjuan Wang, Yixiao Wang, Lanping Ding. Effects of main ecological factors on the growth of marine green alga Caulerpa sertularioides using the response surface methodology[J]. Acta Oceanologica Sinica, 2023, 42(11): 90-97. doi: 10.1007/s13131-023-2171-0

doi: 10.1007/s13131-023-2171-0

Effects of main ecological factors on the growth of marine green alga Caulerpa sertularioides using the response surface methodology

Funds: The National Natural Science Foundation of China under contract Nos 31970216, 32270219 and 31670199.
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  • Figure  1.  The external morphology of Caulerpa sertularioides.

    Figure  2.  The field population of Caulerpa sertularioides in the intertidal zone of Yinggehai, Hainan.

    Figure  3.  The SGR of Caulerpa sertularioides in different irradiances.

    Figure  4.  The SGR of Caulerpa sertularioides in different temperatures.

    Figure  5.  The SGR of Caulerpa sertularioides in different salinities.

    Figure  6.  SGR of Caulerpa sertularioides affected by irradiance.

    Figure  7.  SGR of Caulerpa sertularioides affected by temperature.

    Figure  8.  SGR of Caulerpa sertularioides affected by salinity.

    Figure  9.  SGR of Caulerpa sertularioides affected by irradiance and temperature.

    Figure  10.  SGR of Caulerpa sertularioides affected by irradiance and salinity.

    Figure  11.  SGR of Caulerpa sertularioides affected by temperature and salinity.

    Table  1.   Levels of variables in the Box-Behnken experimental design

    FactorIrradiance /(μmol·m−2·s−1)Temperature/℃Salinity
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    Table  2.   Combinations of the Box-Behnken experimental design

    Serial numberIrradiance/
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    Table  3.   Analysis of variance

    SourceSum of squaresdfMean squareF valueP valueSignificance
    Model12.8991.43304.92<0.000 1Significant
    A−irradiance1.6711.67355.29<0.000 1
    B−temperature0.410.485.16<0.000 1
    AB0.1110.1123.760.001 8
    A23.413.4724.44<0.000 1
    B23.7413.74796.58<0.000 1
    C22.3412.34498.31<0.000 1
    Residual0.03374.70 × 10−3
    Lack of fit9.07 × 10−333.02 × 10−30.510.80Insignificant
    Pure error0.02445.95 × 10−3
    Cor total12.9316
    Note: df: Degrees of Freedom. Cor total: This row shows the amount of variation around the mean of the observations. The model explains part of it, the residual explains the rest.
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  • Anderson L W J. 2005. California’s reaction to Caulerpa taxifolia: a model for invasive species rapid response. Biological Invasions, 7(6): 1003–1016. doi: 10.1007/s10530-004-3123-z
    Bischof K, Hanelt D, Wiencke C. 2000. Effects of ultraviolet radiation on photosynthesis and related enzyme reactions of marine macroalgae. Planta, 211(4): 555–562. doi: 10.1007/s004250000313
    Cai Yixun. 2021. Effect of environmental factors on growth, physiological and biochemical characteristics of Caulerpa lentillifera (in Chinese)[dissertation]. Guangzhou: South China University of Technology
    Carruthers T J B, Walker D I, Huisman J M. 1993. Culture studies on two morphological types of Caulerpa (Chlorophyta) from Perth, Western Australia, with a description of a new species. Botanica Marina, 36(6): 589–596
    Chaves Filho G P, Batista L A N C, de Medeiros S R B, et al. 2022. Sulfated glucan from the green seaweed Caulerpa sertularioides inhibits adipogenesis through suppression of adipogenic and lipogenic key factors. Marine Drugs, 20(8): 470. doi: 10.3390/md20080470
    da Silva L P, Pereira E, Prieto M A, et al. 2019. Rubus ulmifolius Schott as a novel source of food colorant: extraction optimization of coloring pigments and incorporation in a bakery product. Molecules, 24(11): 2181. doi: 10.3390/molecules24112181
    Davis A R, Roberts D E, Cummins S P. 1997. Rapid invasion of a sponge-dominated deep-reef by Caulerpa scalpelliformis (Chlorophyta) in Botany Bay, New South Wales. Austral Ecology, 22(2): 146–150. doi: 10.1111/j.1442-9993.1997.tb00653.x
    de Gaillande C, Payri C, Remoissenet G, et al. 2017. Caulerpa consumption, nutritional value and farming in the Indo-Pacific region. Journal of Applied Phycology, 29(5): 2249–2266. doi: 10.1007/s10811-016-0912-6
    Dennison W C. 1987. Effects of light on seagrass photosynthesis, growth and depth distribution. Aquatic Botany, 27(1): 15–26. doi: 10.1016/0304-3770(87)90083-0
    Ding Lanping, Huang Bingxin, Luan Rixiao. 2015. New classification system of marine green algae of China. Guangxi Sciences (in Chinese), 22(2): 201–210
    Ebadi S, Ashaari Z, Late Masoumi H, et al. 2019. Evaluation of bending strength of hydrothermally treated oil palm wood in various buffered media using response surface methodology. Journal of Tropical Forest Science, 31(2): 151–161. doi: 10.26525/jtfs2019.31.2.151161
    Faramarzi S, Anzabi Y, Jafarizadeh-Malmiri H. 2019. Selenium supplementation during fermentation with sugar beet molasses and Saccharomyces cerevisiae to increase bioethanol production. Green Processing and Synthesis, 8(1): 622–628. doi: 10.1515/gps-2019-0032
    Feng Lina, Shi Xiaoyong, Chen Yuehong, et al. 2021. Effects of temperature on the nitrate reductase activity and growth of Ulva prolifera. Journal of Phycology, 57(3): 955–966. doi: 10.1111/jpy.13141
    Fernández C, Cortés J. 2005. Caulerpa sertularioides, a green alga spreading aggressively over coral reef communities in Culebra Bay, North Pacific of Costa Rica. Coral Reefs, 24(1): 10. doi: 10.1007/s00338-004-0440-8
    Flexas J, Bota J, Loreto F, et al. 2004. Diffusive and metabolic limitations to photosynthesis under drought and salinity in C3 plants. Plant Biology, 6(3): 269–279. doi: 10.1055/s-2004-820867
    Gao Kunshan. 2014. Algal Carbon Fixation: Basis, Advances and Methods (in Chinese). Beijing: Science Press
    Gong Shusen, Yang Fei, Wang Qingfen, et al. 2022. Processing of Noni Liquor based on response surface methodology. PeerJ, 10: e13817. doi: 10.7717/peerj.13817
    Guo Hui, Yao Jianting, Sun Zhongmin, et al. 2015. Effects of salinity and nutrients on the growth and chlorophyll fluorescence of Caulerpa lentillifera. Chinese Journal of Oceanology and Limnology, 33(2): 410–418. doi: 10.1007/s00343-015-4105-y
    Hadiyat M A, Sopha B M, Wibowo B S. 2022. Response surface methodology using observational data: a systematic literature review. Applied Sciences, 12(20): 10663. doi: 10.3390/app122010663
    Huang Jianhui. 2012. Effects of concentrations of nitrogen and phosphorus and different culture methods on the growth of Caulerpa lentillifera. Journal of Fujian Fisheries (in Chinese), 34(5): 416–419
    Jiang Haiyan, Wu Sichao, Cen Yingzhou. 2011. Nutrient component analysis of six kinds of economic algae in Zhanjiang sea area. Guangzhou Chemical Industry (in Chinese), 39(7): 105–108
    Kang E J, Lee S, Kang J, et al. 2021. Performance of a potentially invasive species of ornamental seaweed Caulerpa sertularioides in acidifying and warming oceans. Journal of Marine Science and Engineering, 9(12): 1368. doi: 10.3390/jmse9121368
    Kim M, Lee B, Kim H S, et al. 2019. Increased biomass and lipid production of Ettlia sp. YC001 by optimized C and N sources in heterotrophic culture. Scientific Reports, 9(1): 6830. doi: 10.1038/s41598-019-43366-5
    Klein J C, Verlaque M. 2008. The Caulerpa racemosa invasion: a critical review. Marine Pollution Bulletin, 56(2): 205–225. doi: 10.1016/j.marpolbul.2007.09.043
    Kolar C S, Lodge D M. 2001. Progress in invasion biology: predicting invaders. Trends in Ecology & Evolution, 16(4): 199–204
    Kramar A, Turk S, Vrečer F. 2003. Statistical optimisation of diclofenac sustained release pellets coated with polymethacrylic films. International Journal of Pharmaceutics, 256(1–2): 43–52. doi: 10.1016/S0378-5173(03)00061-9
    Kumar M, Gupta V, Kumari P, et al. 2011. Assessment of nutrient composition and antioxidant potential of Caulerpaceae seaweeds. Journal of Food Composition and Analysis, 24(2): 270–278. doi: 10.1016/j.jfca.2010.07.007
    Landi S, Santini G, Vitale E, et al. 2022. Photosynthetic, molecular and ultrastructural characterization of toxic effects of zinc in Caulerpa racemosa indicate promising bioremediation potentiality. Plants, 11(21): 2868. doi: 10.3390/plants11212868
    Lapointe B E, Barile P J, Wynne M J, et al. 2005. Reciprocal Caulerpa invasion: Mediterranean native Caulerpa ollivieri in the Bahamas supported by human nitrogen enrichment. Aquatic Invaders, 16(2): 2–5
    Li Tianjingwei, Ma Bin, Zou Xiaoxiao, et al. 2022. Physiological mechanism of response to temperature stress in seaweed Caulerpa lentillifera. Chinese Journal of Fisheries (in Chinese), 35(4): 67–73
    Liu Rui, Li Yongmei, Wang Yixiao, et al. 2019. Biogeographical distribution, biological characteristics and biological invasion of Caulerpa (Chlorophyta) seaweeds. Journal of Anhui Agricultural Sciences (in Chinese), 47(4): 95–98, 104
    Liu Hengtong, Wang Fang, Wang Qiaohan, et al. 2016. A comparative study of the nutrient uptake and growth capacities of seaweeds Caulerpa lentillifera and Gracilaria lichenoides. Journal of Applied Phycology, 28(5): 3083–3089. doi: 10.1007/s10811-016-0858-8
    Mäkelä M. 2017. Experimental design and response surface methodology in energy applications: a tutorial review. Energy Conversion and Management, 151: 630–640. doi: 10.1016/j.enconman.2017.09.021
    Mee R W. 2009. A Comprehensive Guide to Factorial Two-Level Experimentation. New York: Springer
    Meinesz A, Belsher T, Thibaut T, et al. 2001. The introduced green alga Caulerpa Taxifolia continues to spread in the mediterranean. Biological Invasions, 3(2): 201–210. doi: 10.1023/A:1014549500678
    Nazzal S, Nutan M, Palamakula A, et al. 2002. Optimization of a self-nanoemulsified tablet dosage form of ubiquinone using response surface methodology: effect of formulation ingredients. International Journal of Pharmaceutics, 240(1–2): 103–114. doi: 10.1016/S0378-5173(02)00130-8
    Nur M M A, Garcia G M, Boelen P, et al. 2019. Enhancement of C-phycocyanin productivity by Arthrospira platensis when growing on palm oil mill effluent in a two-stage semi-continuous cultivation mode. Journal of Applied Phycology, 31(5): 2855–2867. doi: 10.1007/s10811-019-01806-9
    Okur N, Çengel M, Göçmez S. 2002. Influence of salinity on microbial respiration and enzyme activity of soils. Acta Horticulturae, (573): 189–194
    Pang Meixia, Huang Zhili, Liu Xiaolong, et al. 2021. Effects of heavy metal cadmium on Caulerpa lentillifera based on transcriptome analysis. Chinese Journal of Applied Ecology (in Chinese), 32(12): 4447–4456
    Piazzi L, Ceccherelli G, Cinelli F. 2001. Threat to macroalgal diversity: effects of the introduced green alga Caulerpa racemosa in the Mediterranean. Marine Ecology Progress Series, 210: 149–159. doi: 10.3354/meps210149
    Piazzi L, Cinelli F. 1999. Développement et dynamique saisonnière d'un peuplement méditerranéen de l′algue tropicale Caulerpa racemosa (Forsskl) J. Agardh. Cryptogamie Algologie, 20(4): 295–300. doi: 10.1016/S0181-1568(00)88146-5
    Pinheiro A M, Maia G A, Figueiredo R W, et al. 2022. Response surface methodology optimization of blended fruit nectar: cashew apple and açai. International Journal of Fruit Science, 22(1): 275–286. doi: 10.1080/15538362.2022.2037037
    Ruitton S, Verlaque M, Boudouresque C F. 2005. Seasonal changes of the introduced Caulerpa racemosa var. cylindracea (Caulerpales, Chlorophyta) at the northwest limit of its Mediterranean range. Aquatic Botany, 82(1): 55–70. doi: 10.1016/j.aquabot.2005.02.008
    Shevchenko N M, Burtseva Y V, Zvyagintseva T N, et al. 2009. Polysaccharides and sterols from green algae Caulerpa lentillifera and C. sertularioides. Chemistry of Natural Compounds, 45(1): 1–5. doi: 10.1007/s10600-009-9223-3
    Shi Jianhong. 2008. Field survey and culture studies of Caulerpa in Taiwan (in Chinese)[dissertation]. Taipei: National Sun Yat-Sen University
    Shi Xiaohan, Zou Dinghui, He Quan, et al. 2022. Photophysiological characteristics of the branch and stolon of macroalga Caulerpa lentillifera (Caulerpaceae, Caulerpa) under different growth light conditions, and their responses to temperature rise. Journal of Tropical Oceanography (in Chinese), 41(5): 150–160
    Smith T B, Fong P, Kennison R, et al. 2010. Spatial refuges and associational defenses promote harmful blooms of the alga Caulerpa sertularioides onto coral reefs. Oecologia, 164(4): 1039–1048. doi: 10.1007/s00442-010-1698-x
    Smith C M, Walters L J. 1999. Fragmentation as a strategy for Caulerpa species: fates of fragments and implications for management of an invasive weed. Marine Ecology, 20(3–4): 307–319. doi: 10.1046/j.1439-0485.1999.2034079.x
    Srinivas G, Nidoni U, Ramachandra C T, et al. 2019. Supercritical fluid extraction of pupae oil from mulberry silkworm (Bombyx mori L.). Journal of Pharmacognosy and Phytochemistry, 8(3): 4507–4513
    Stensrud G, Sande S A, Kristensen S, et al. 2000. Formulation and characterisation of primaquine loaded liposomes prepared by a pH gradient using experimental design. International Journal of Pharmaceutics, 198(2): 213–228. doi: 10.1016/S0378-5173(00)00338-0
    Stuthmann L E, Springer K, Kunzmann A. 2021. Cultured and packed sea grapes (Caulerpa lentillifera): effect of different irradiances on photosynthesis. Journal of Applied Phycology, 33(2): 1125–1136. doi: 10.1007/s10811-020-02322-x
    Tong Yanmei, Chen Xiuli, Hu Tingjun, et al. 2022. Optimization of extraction process and antioxidant activity of polysaccharides from Caulerpa lentillifera by response surface methodology. Journal of Fisheries of China (in Chinese). https://kns.cnki.net/kcms/detail/31.1283.S.20220518.1019.004.html[2022-05-18/2022-12-20]
    Verlaque M, Afonso-Carrillo J, Gil-Rodríguez M C, et al. 2004. Blitzkrieg in a marine invasion: Caulerpa racemosa var. cylindracea (Bryopsidales, Chlorophyta) reaches the Canary Islands (north-east Atlantic). Biological Invasions, 6(3): 269–281. doi: 10.1023/B:BINV.0000034589.18347.d3
    Vishwakarma R, Banerjee R. 2019. Process optimization for enhancement of fermentable sugar from Cyperus sp. through enzymatic saccharification. Journal of Biofuels, 10(1): 1–11
    Wang Zewen. 2010. Study on the determination of seaweed growth regulator and fertilizer efficiency (in Chinese)[dissertation]. Qingdao: Ocean University of China
    Wang Zhan. 2015. Preliminary studies on environmental adaptation and paling mechanism of Caulerpa (in Chinese)[dissertation]. Shantou: Shantou University
    Wang Zonghua, Sun Na, He Yao, et al. 2014. DNA assembled gold nanoparticles polymeric network blocks modular highly sensitive electrochemical biosensors for protein kinase activity analysis and inhibition. Analytical Chemistry, 86(12): 6153–6159. doi: 10.1021/ac501375s
    Wells C M, Di Cera E. 1992. Thrombin is a sodium ion activated enzyme. Biochemistry, 31(47): 11721–11730. doi: 10.1021/bi00162a008
    Williams S L. 1984. Uptake of sediment ammonium and translocation in a marine green macroalga Caulerpa cupressoides. Limnology and Oceanography, 29(2): 374–379. doi: 10.4319/lo.1984.29.2.0374
    Williams S L, Schroeder S L. 2004. Eradication of the invasive seaweed Caulerpa taxifolia by chlorine bleach. Marine Ecology Progress Series, 272: 69–76. doi: 10.3354/meps272069
    Withgott J. 2002. California tries to rub out the monster of the lagoon. Science, 295(5563): 2201–2202. doi: 10.1126/science.295.5563.2201
    Xu Ning, Duan Shunshan, Li Aifen, et al. 2010. Effects of temperature, salinity and irradiance on the growth of the harmful dinoflagellate Prorocentrum donghaiense Lu. Harmful Algae, 9(1): 13–17. doi: 10.1016/j.hal.2009.06.002
    Yu Ping. 2005. Effect of temperature, irradiance and population interaction on the growth of phytoplankton of East China Sea (in Chinese)[dissertation]. Qingdao: Ocean University of China
    Zhang Meijian, Ma Yurong, Che Xinyi, et al. 2020. Comparative analysis of nutrient composition of Caulerpa lentillifera from different regions. Journal of Ocean University of China, 19(2): 439–445. doi: 10.1007/s11802-020-4222-x
    Zhao Yuping, Chen Xiaowang, Shen Pengwei. 2013. Optimization of culture medium to produce manganese peroxidase by using Box-Behnken experimental design and response surface methodology. Science and Technology of Food Industry (in Chinese), 34(4): 207–211
    Zhong Yiyun, Yang Yunqi, Gao Xiaofeng, et al. 2021. Effects of salinity, temperature and light intensity on growth and photosynthetic activity of Caulerpa sertularioides. Journal of Tropical and Subtropical Botany (in Chinese), 29(6): 626–633
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  • 收稿日期:  2022-10-25
  • 录用日期:  2023-01-11
  • 网络出版日期:  2023-09-18
  • 刊出日期:  2023-11-01