Parentage determination of black sea bream (Acanthopagrus schlegelii) for stock enhancement: Effectiveness and loss of genetic variation

Binbin Shan Yan Liu Na Song Changping Yang Shengnan Liu Tianxiang Gao Dianrong Sun

Binbin Shan, Yan Liu, Na Song, Changping Yang, Shengnan Liu, Tianxiang Gao, Dianrong Sun. Parentage determination of black sea bream (Acanthopagrus schlegelii) for stock enhancement: Effectiveness and loss of genetic variation[J]. Acta Oceanologica Sinica. doi: 10.1007/s13131-020-1697-7
Citation: Binbin Shan, Yan Liu, Na Song, Changping Yang, Shengnan Liu, Tianxiang Gao, Dianrong Sun. Parentage determination of black sea bream (Acanthopagrus schlegelii) for stock enhancement: Effectiveness and loss of genetic variation[J]. Acta Oceanologica Sinica. doi: 10.1007/s13131-020-1697-7

doi: 10.1007/s13131-020-1697-7

Parentage determination of black sea bream (Acanthopagrus schlegelii) for stock enhancement: Effectiveness and loss of genetic variation

Funds: Science and Technology Project of Guangdong Province under contract No. 2019B121201001; National Key R&D Program of China under contract No. 2019YFD0901301; the Fund of China-Vietnam Joint Survey on Fish Stocks in the Common Fishing Zone of the Beibu Gulf; Central Public-interest Scientific Institution Basal Research Fund, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science under contract No. 2021SD14; China-ASEAN Maritime Cooperation Fund (China-Vietnam Fishery Stock Enhancement and Conservation in Beibu Gulf).
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  • Figure  1.  The approximate location of sampling localities.

    Figure  2.  The distribution of the broodfish’s contribution to offspring.

    Figure  3.  The numbers of offspring and body length of breeders.

    Figure  4.  Standard length frequency distribution of A. schlegelii.

    Table  1.   Information about the releasing

    Releasing date (yy/mm/dd)Releasing siteReleasing numberBody length/mmBody weight/g
    2015/05/22ZRE73 00030.430.71
    2015/06/29 ZRE17 00040.422.02
    2015/06/29 DB18 000 40.422.02
    2015/07/29DB5 50080.7817.97
    Note: ZRE, Zhujiang River Esturay, DB, Daya Bay.
    下载: 导出CSV

    Table  2.   Sample information of A. schlegelii

    Recapture time (yy/mm/dd)Recapture siteNumber of total samplesNumber of genotyped individuals
    2015/08/16–2015/08/24ZRE17296
    2015/08/03–2015/08/26DB12396
    2015/09/04ZRE10397
    2015/10/01ZRE203203
    2015/12/01ZRE4242
    2016/03ZRE2828
    2016/04ZRE2121
    Total692583
    Note: ZRE, Zhujiang River Esturay; DB, Daya Bay; and –, no data.
    下载: 导出CSV

    Table  3.   Parameters of microsatellite loci in identification of hatchery-released individuals and parentage assignments

    LocusMeanCombine NE-PP
    Asca1Asca3Asca6Asca4Asca16Asca17
    Parentage assignmentsNE-PP0.5770.0880.3980.2510.0770.1010.000 03
    PIC0.4260.8670.5760.7420.8780.8570.724
    Identification of hatchery-released individualsNE-PP0.3390.0660.2510.2080.1480.0790.000 013
    PIC0.6390.8860.690.7750.8080.8770.779
    Note: PIC, Polymorphic information content; NE-PP, Average non-exclusion probability for a candidate parent pair.
    下载: 导出CSV

    Table  4.   The quantity and percentage of released-recaptured A. schlegelii in samples

    Recapture date (yy/mm/dd)Recapture siteNumber of genotyped individualsNumber of released-recaptured fishRate
    2015/08/16–2015/08/24ZRE963334.4%
    2015/08/03–2015/08/26DB961515.6%
    2015/09/04ZRE972121.6%
    2015/10/01ZRE2035326.1%
    2015/12/01ZRE421126.2%
    2016/03ZRE28621.4%
    2016/04ZRE21419.0%
    Total58314324.5%
    下载: 导出CSV

    Table  5.   Genetic variability in broodfish, hatchery-released fish, recaptured fish

    nAARHEHoIFisHWE
    Broodfish936911.1080.7740.7541.8820.027NS
    Hatchery-released fish141619.2850.7270.7581.706–0.046
    WZRE35911515.1190.8200.7432.1390.096
    RZRE48711514.4690.8030.7402.0710.083
    WDB81599.7550.7330.7871.628–0.117
    RDB96599.4610.7240.7801.605–0.113
    Note: RZRE, recaptured fish in Zhujiang River Estuary; WZRE, wild fish in recaptured fish in Zhujiang River Estuary; RDB, recaptured fish in Daya Bay; and WDB, wild fish in recaptured fish in Daya Bay. A, number of alleles, AR, allele richness; HE, heterozygosity alleles; I, Shannon Index; HWE, deviations from Hardy-Weinberg equilibrium; n, sample size; Ho, observed heterozygosity; Fis, fixation index; NS, P < 0.05; *, P < 0.05; and –, no data.
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    Table  6.   Estimates of FST (below diagonal) and genetic distance (above diagonal) value

    BroodstockHatchery-reared fishWZRERZREWDBRDB
    Broodstock0.0260.0810.0610.1050.097
    Hatchery-released fish0.004*0.0970.0740.1180.108
    WZRE0.025*0.034*0.0030.1380.132
    RZRE0.015*0.022*0.001*0.1200.113
    WDB0.039*0.049*0.0410.034*0.002
    RDB0.035*0.044*0.040*0.032*–0.005
    Note: RZRE, recaptured fish in Zhujiang River Estuary; WZRE, wild fish in recaptured fish in Zhujiang River Estuary; RDB, recaptured fish in Daya Bay; and WDB, wild fish in recaptured fish in Daya Bay. *, P < 0.05; and –, no data.
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  • 收稿日期:  2019-11-28
  • 录用日期:  2020-06-03
  • 网络出版日期:  2021-06-21

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