Diversity of protease-producing bacteria in the Bohai Bay sediment and their extracellular enzymatic properties

Zhenpeng Zhang Chaoya Wu Shuai Shao Wei Liu En-Tao Wang Yan Li

Zhenpeng Zhang, Chaoya Wu, Shuai Shao, Wei Liu, En-Tao Wang, Yan Li. Diversity of protease-producing bacteria in the Bohai Bay sediment and their extracellular enzymatic properties[J]. Acta Oceanologica Sinica. doi: 10.1007/s13131-020-1589-x
Citation: Zhenpeng Zhang, Chaoya Wu, Shuai Shao, Wei Liu, En-Tao Wang, Yan Li. Diversity of protease-producing bacteria in the Bohai Bay sediment and their extracellular enzymatic properties[J]. Acta Oceanologica Sinica. doi: 10.1007/s13131-020-1589-x

doi: 10.1007/s13131-020-1589-x

Diversity of protease-producing bacteria in the Bohai Bay sediment and their extracellular enzymatic properties

Funds: The National Natural Science Foundation of China under contract Nos 31600009 and 31800099; the Key Research and Development Program of Hebei Province under contract No. 19273802D; the STS Program of Chinese Academy of Sciences and Fujian Province under contract No. 2017T3019; the Yantai Science and Technology Project under contract No. 2018ZHGY074; the Joint Fund of Jilin Province and Chinese Academy of Sciences for High-tech Industrialization under contract No. 2019SYHZ0036; En-Tao Wang was supported by the projects under contract Nos SIP 20150597 and 20160883 authorized by IPN, Mexico.
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  • Figure  1.  Map of the Bohai Bay in the Bohai Sea of China showing the sampling sites (●). The map was created using DIVA-GIS software (http//www.diva-gis.org), and the sampling sites were added according to GPS records.

    Figure  2.  Phylogenetic tree of the protease-producing bacteria isolated from the Bohai Bay based on 16S rRNA gene sequences. Taxa and GenBank accession numbers in boldface were generated in this study. The tree was constructed by neighbor-joining method using MEGA version 6.0. Only bootstrap values greater than 50% are presented in the nodes. The scale bar represents 2% nucleotide substitution.

    Table  1.   Characteristics of the sampling stations and the distribution of different genera in these stations

    PropertiesStation
    BH07BH12BH17BH18BH20BH22CFD39
    GPS38.35°N, 118.75°E38.55°N, 117.95°E38.55°N, 118.95°E38.75°N, 118.95°E38.75°N, 118.55°E38.75°N, 118.15°E39.07°N, 118.53°E
    Characteristics for sediment samples
    Depth/m16.149.6622.6525.5723.7817.2712.19
    Temperature/°C24.9727.1120.3619.3223.2725.4726.14
    pH8.118.087.917.908.018.188.08
    DO4.024.172.202.283.505.114.86
    Sal30.3030.0230.3130.4530.5130.2030.63
    OrgC/%0.420.510.550.600.540.570.87
    OrgN/%0.060.080.090.080.090.100.11
    C/N1)7.006.386.117.56.005.707.91
    Genera distribution
    ProteobacteriaPseudoalteromonas381561678
    Shewanella31
    Marinobacter13
    Sulfitobacter44
    Celeribacter111
    Albirhodobacter11
    FirmicutesBacillus711
    Halobacillus111
    Fictibacillus1
    ActinobacteriaMicrococcus11
    Citricoccus1
    Brachybacterium1
    BacteroidetesArenibacter 2
    Salegentibacter222
    Total strain number (109)
    Diversity index
    Shannon–Wiener (H′)1.581.221.661.010.340.940.64
    Simpson (D)0.720.610.710.610.200.480.34
    Pielou (J)0.810.760.760.920.500.680.58
    Note: 1) C/N is the abbreviation of OrgC/OrgN.
    下载: 导出CSV

    Table  2.   Summary of the inhibition test and the extracellular enzyme production analyses of the tested strains isolated from the Bohai Bay

    GeneraStrainInhibition ratio1) (I)/%H/C ratio2)Nitrate
    PMSFO-PE-64P-ACaseinGelatinElastinAmylaseCellulaseAlginaseTween 80
    Pseudoalteromonas7000443.6422.87001.773.001.270000
    7000660.9703.3502.782.921.404.002.183.266.31+
    7003280.7546.83001.543.1000001.70
    7003816.890002.334.5000001.61
    7003943.830002.504.71003.681.113.27
    7004848.4116.53002.785.0000004.52+
    7005171.3545.1902.993.252.671.860002.59
    7005376.4329.47002.255.000001.495.40
    7005680.050002.784.140003.600
    7030546.370003.421.402.211.5901.203.17
    7030649.1834.794.1102.873.221.581.761.081.251.51+
    7030781.1044.84002.942.561.923.2301.333.36
    7030899.2348.205.8002.752.871.582.0701.163.83+
    7032052.135.81002.311.461.551.491.221.221.51
    7032539.576.21003.141.271.701.9801.442.99+
    7032681.4234.55003.191.142.102.1901.961.70
    7033329.636.34002.621.201.551.6401.202.79
    7033990.1331.284.484.482.0001.501.614.111.233.46+
    7034285.923.20002.181.451.580000
    7034470.0453.847.0402.381.421.251.3401.282.96
    7035451.0420.354.2503.432.851.731.2601.321.33
    7035668.4010.6007.723.181.572.091.7501.311.19
    7035977.0442.9503.743.421.801.601.8301.862.64+
    7036150.746.173.6003.002.361.771.3502.122.83
    7036592.2927.77003.213.292.501.3402.180+
    7036957.3734.39002.442.081.501.761.412.371.18
    7037395.1944.7903.123.293.171.502.971.123.362.73+
    7037476.8237.19003.132.271.362.3606.552.78
    7037561.0802.2302.932.882.112.8904.630
    7037615.395.92003.002.751.33002.160
    7037942.4651.173.7002.802.641.801.2703.292.79
    7038091.5251.241.284.572.823.151.6401.592.100
    7041399.1832.76002.291.271.331.4002.622.71
    Bacillus7001363.698.67001.803.670008.740
    700359.420001.406.5000000+
    7003719.803.89002.147.000002.962.04
    7004992.5844.7403.094.805.421.320000
    7005531.9818.387.457.631.363.8600000
    Fictibacillus7001443.211.84001.675.000002.520
    Micrococcus7002568.6024.307.8304.005.1700000
    7002761.2617.129.0103.804.140001.880
    Halobacillus7007438.710003.573.332.29002.950
    7007563.5715.94002.004.00001.3700
    Salegentibacter7030435.376.197.2404.601.295.00002.630+
    7039084.240008.337.835.40003.410+
    7040042.390005.004.603.57002.000
    7040146.268.49006.756.005.00002.620+
    7041620.221.31006.003.005.50002.110+
    Marinobacter7043441.7037.85002.09003.5405.272.40+
    7043538.7435.58001.283.1903.8207.842.40+
    Note: 1) Inhibition ratio (I, %) was calculated by using control activity minus the relative activity of a sample with an inhibitor and the activity of a sample without any inhibitor was taken as a control. 2) H/C ratio is the ratio of the hydrolytic zone diameter versus the colony diameter of a colony on the plate. PMSF, phenylmethylsulfonyl fluoride; OP, 1, 10-phenanthroline; P-A, pepstatin A.
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  • 收稿日期:  2019-05-02
  • 录用日期:  2019-06-24

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