Frequent recombination in <i>Cynoglossus</i> <i>abbreviatus</i> (Pleuronectiformes: Cynoglossidae) ribosomal 18S rDNA

Li Gong; Tingqi Jiang; Bilin Hu; Kaixin Wang; Nannan Zhang; Zengliang Miao

doi:10.1007/s13131-024-2291-1

Volume 43 Issue 8

Aug. 2024

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Article Navigation > Acta Oceanologica Sinica > 2024 > 43(8): 98-103

Li Gong, Tingqi Jiang, Bilin Hu, Kaixin Wang, Nannan Zhang, Zengliang Miao. Frequent recombination in Cynoglossus abbreviatus (Pleuronectiformes: Cynoglossidae) ribosomal 18S rDNA[J]. Acta Oceanologica Sinica, 2024, 43(8): 98-103. doi: 10.1007/s13131-024-2291-1

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Li Gong, Tingqi Jiang, Bilin Hu, Kaixin Wang, Nannan Zhang, Zengliang Miao. Frequent recombination in Cynoglossus abbreviatus (Pleuronectiformes: Cynoglossidae) ribosomal 18S rDNA[J]. Acta Oceanologica Sinica, 2024, 43(8): 98-103. doi: 10.1007/s13131-024-2291-1

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Frequent recombination in Cynoglossus abbreviatus (Pleuronectiformes: Cynoglossidae) ribosomal 18S rDNA

doi: 10.1007/s13131-024-2291-1

Li Gong^{1, 2},
Tingqi Jiang^{1, 2},
Bilin Hu^{1, 2},
Kaixin Wang^{1, 2},
Nannan Zhang^{1, 2},
Zengliang Miao^{1, 2
,
,}

1.
National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Zhejiang Ocean University, Zhoushan 316022, China
2.
Marine Science and Technology College, Zhejiang Ocean University, Zhoushan 316022, China

Funds: The Basic Scientific Research Operating Expenses of Zhejiang Provincial Universities under contract 2021JZ003; the Zhoushan Science and Technology Bureau under contract No. 2021C21007; the Natural Science Foundation of Zhejiang Province under contract Y21C190023; the National Natural Science Foundation of China under contract 31272273.

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Corresponding author: miaozl@zjou.edu.cn
Received Date: 2023-09-22
Accepted Date: 2023-11-11

Available Online: 2024-04-22

Publish Date: 2024-08-25

Abstract

Abstract

The conventional theory of concerted evolution has been used to explain the lack of sequence variation in ribosomal RNA (rRNA) genes across diverse eukaryotic species. However, recent investigations into rRNA genes in flatfish genome have resulted in controversial findings. This study focuses on 18S rRNA genes of the widely distributed tongue sole, Cynoglossus abbreviatus (Pleuronectiformes: Cynoglossidae), aiming to explore sequence polymorphism. Five distinct 18S rDNA sequence types (Type A, B, R1, R2, and R3) were identified, suggesting a departure from concerted evolution. A combination of general criteria and variations in highly conserved regions were employed to detect pseudogenes. The results pinpointed Type A sequences as potential pseudogenes due to significant sequence variations and deviations in secondary structure within highly conserved regions. Three types (Type R1, R2, and R3) were identified as recombinants between Type A and B sequences, with simple crossing over and gene conversion as the most likely recombination mechanisms. These findings not only contribute to rRNA pseudogene identification but also shed light on the evolutionary dynamics of rRNA genes in teleost genomes.
- ribosomal RNA,
- tongue sole,
- non-concerted evolution,
- pseudogene,
- crossing over,
- gene conversion

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