Tracing technique of stable ¹⁵N isotope: an application in study of mariculture

Using Na¹⁵NO₃ and ¹⁵SNH₄Cl as tracer, we studied the transfer of ¹⁵N along the food chain composed of organisms such as Platymonas subcordifwmis, Anemia saliva, Perwt;u perdcillatus and Liza carinatus.The abundance and atomic percentage of ¹⁵N in each organism, as well as the transfer of ¹⁵N among different biological phases were measured.Results show that, in the nutrient transfer from low level to high level along the food chain in the media of ¹⁵SNH₄Cl, the transfer rate of Anemia saliva→Liza casinatus exceeded 3% and was higher than that in Na Na¹⁵NO₃;the accumulation of ¹⁵N in the bodies of Lira carinatvs with ¹⁵SNH,Cl as media was also higher than that with Na Na¹⁵NO₃ as media.