Cytochalasin B induced triploidy in Penaeus chinensis

Fertilized eggs of Penaerrs chinenris were treated with cytochalasin B (C.B) to induce triploid.A number of them were cultured to the stage of zoea larvae.The C.B concentrations employed were 0.1~2.5mg/dm³.The treatments started at 5-20 min after fertilisation and lasted for various periods of time from 10 to 40 min.The highest inducing rate was 62.5%.It was obvious that higher C.B concentration gave stronger inhibition on the release of polar bodies,and the higher the C.B concentration wes, the more abnormal and aneuploid embryos were obtained.The sucteas in inducing triploid in P.chnrruis provided a posibility of polyploid breeding of this species.