The total inline wave forces, the irregular wave forces in particular, on an isolated pile are investigated by experiment.The relationships between force coefficients Cd and CM including in Morison's Eq., and KC number or Reynolds number Re, and the variation of Cd and Cm in frequency domain are analysed with the method of least-squares in time domain and that of cross-spectral analysis.The plots of C4 and Cm versus KC are given for both regular and irregular waves and those for irregular waves are used for numerical simulation of the irregular wave forces on the vertical pile and the results are in fairly good agreement with the test data.Based on the experimental results, the applicability of the spectral analysis method for calculating irregular wave forces on an isolated pile is investigated with the coherency γ between wave and wave forces and with KC number.