ZHU RENHUA, SHI HUIXIONG, LIU RUGAO. A STUDY OF ALGINATE LYASES Ⅱ. THEIR EFFECTS ON SUBSTRATE[J]. Acta Oceanologica Sinica, 1987, (3): 434-443.
Citation: ZHU RENHUA, SHI HUIXIONG, LIU RUGAO. A STUDY OF ALGINATE LYASES Ⅱ. THEIR EFFECTS ON SUBSTRATE[J]. Acta Oceanologica Sinica, 1987, (3): 434-443.

A STUDY OF ALGINATE LYASES Ⅱ. THEIR EFFECTS ON SUBSTRATE

  • Received Date: 1986-03-19
  • Rev Recd Date: 1986-07-03
  • Two acid partial hydrolysis methods are used in preparing three substrates, i. e. short-chain poly-mannuronide (SM), short-chain polyguluronide (SG) and short-chain polymannuronide-polyguluronide (SMG).
    Using viscosity reduction method, it is demonstrated that lyase Ⅰ is an exolyase while lyase Ⅱ and lyase Ⅲ are endolyases.
    Somogyi-Nelson method is used for determining the reducibility of lyase-decomposed substrates, indicating that the three lyases acted strongly on algin, SM and SMG, but slightly on SO. UV absorption method is used for determining absorption spectrum of products, indicating that all the three lyases are polymannuronide lyases (EC 4·2·99·4).
    With their aigins decomposed, all the three lyases can reduce the M/G value, the best being lyase I which can reduce the M/G by about 3.65 fola. Therefore, the enzymatic method might be used in preparing medicine for preventive absorption of 90Sr.
  • loading
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (373) PDF downloads(94) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return