The method of acoustic backscattering measurement from different parts of fish using the focus array of transducer developed recently is described in this paper. The results of the measurements of several kinds of flsh are given in this paper, indicating that the backscattering from fish is closely relative to the anatomy of fish. Its application in forming backscattering model of fish and that in using this model to obtain the information of fish kinds by the echoes from fish sonar are discussed.