In this paper the chemical characteristics of humic substances (humic acid and fulvic acid) from East China Sea surficial sediment are studied through combination of chemical and physical methods.Results show that humic acids have less aromatic hydrocarbon and more alkane structure. The contents of amino acids from hydrolyzate of humic substances are different.The amount of various amino acids (such as acidic,neutral or basic amino aicd) of fulvic acid is higher than that of humic acid.The distribution order of amino acid abundance in various kinds of sediments is neutral amino acid > aromatic amino acid > basic amino acid. In addition,mathematical statistic method is also used to study their distribution.All samples show similar regularities,i.e.,the contents of glycine,alanine,aspartic and glutamic are very high and those of cystine and methionine are very low or even disappear. The above results further confirm that humic acid and fulvic acid have similar composition and structure.