REPRODUCTIVE MECHANISM OF RUDITAPES PHILIPPINARUM ISOLATION OF SHEDDING HORMONE AND DETERMINATION OF BIOLOGICAL ACTIVITY

FANG YONGQIANG; ZHANG YUSHENG; XU RUIAN; ZHANG QISEN

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Article Navigation > Acta Oceanologica Sinica > 1985 > (1): 107-112

FANG YONGQIANG, ZHANG YUSHENG, XU RUIAN, ZHANG QISEN. REPRODUCTIVE MECHANISM OF RUDITAPES PHILIPPINARUM ISOLATION OF SHEDDING HORMONE AND DETERMINATION OF BIOLOGICAL ACTIVITY[J]. Acta Oceanologica Sinica, 1985, (1): 107-112.

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REPRODUCTIVE MECHANISM OF RUDITAPES PHILIPPINARUM ISOLATION OF SHEDDING HORMONE AND DETERMINATION OF BIOLOGICAL ACTIVITY

Third Institute of Oceanography, National Bureau of Oceanography, Xiamen

Received Date: 1982-11-29
Rev Recd Date: 1984-01-19

Abstract

Abstract

An active substance, shedding hormone capable of inducing parent clams to spawn and sper-miate, has preliminarily been isolated from the ovaries of Ruditapes philippinarum by means of ammonia sulfate precipitate of 25% concentration and Sephadex G-25 column chromatography in present study. The biological activity of the shedding hormone is above 95%. It is composed of sorts of amino acids by determination with an amino acid automatic analyzer. The experimental results indicate that the active substance is a polypeptide.

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