Microbiota of a seepage face at the mouth of a subterranean estuary: diversity, distribution and substrate dependence

Yueming Wu; Yongjun Wei; Juan Severino Pino Ibánhez; Danqing Sun; Kaiqi Lu; Qianqian Wang; Jian Li; Shan Jiang

doi:10.1007/s13131-023-2257-8

Volume 42 Issue 8

Aug. 2023

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Article Navigation > Acta Oceanologica Sinica > 2023 > 42(8): 147-157

Yueming Wu, Yongjun Wei, Juan Severino Pino Ibánhez, Danqing Sun, Kaiqi Lu, Qianqian Wang, Jian Li, Shan Jiang. Microbiota of a seepage face at the mouth of a subterranean estuary: diversity, distribution and substrate dependence[J]. Acta Oceanologica Sinica, 2023, 42(8): 147-157. doi: 10.1007/s13131-023-2257-8

Citation:

Yueming Wu, Yongjun Wei, Juan Severino Pino Ibánhez, Danqing Sun, Kaiqi Lu, Qianqian Wang, Jian Li, Shan Jiang. Microbiota of a seepage face at the mouth of a subterranean estuary: diversity, distribution and substrate dependence[J]. Acta Oceanologica Sinica, 2023, 42(8): 147-157. doi: 10.1007/s13131-023-2257-8

Citation:

Yueming Wu, Yongjun Wei, Juan Severino Pino Ibánhez, Danqing Sun, Kaiqi Lu, Qianqian Wang, Jian Li, Shan Jiang. Microbiota of a seepage face at the mouth of a subterranean estuary: diversity, distribution and substrate dependence[J]. Acta Oceanologica Sinica, 2023, 42(8): 147-157. doi: 10.1007/s13131-023-2257-8

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Microbiota of a seepage face at the mouth of a subterranean estuary: diversity, distribution and substrate dependence

doi: 10.1007/s13131-023-2257-8

1.
State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai 200241, China
2.
School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, China
3.
Instituto de Investigacións Mariñas, Consejo Superior de Investigaciones Científicas, Vigo 36208, Spain
4.
School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen 518055, China
5.
School of Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China

Funds: The National Natural Science Foundation of China under contract No. 41706081.

More Information

Corresponding author: E-mail: sjiang@sklec.ecnu.edu.cn
Received Date: 2023-07-04
Accepted Date: 2023-09-18
Publish Date: 2023-08-31

Abstract

Abstract

Subterranean estuaries, i.e., the mixing zone between terrestrial groundwater and recirculated seawater, host a wide range of microbiota. Here, field campaigns were conducted at the mouth of the subterranean estuary at the Sanggou Bay (Shandong Province, China) over four consecutive seasons at a seepage face (0−20 cm depth). The diversity of benthic microbiome was characterized via 16S rRNA gene sequencing and metagenomics, combined with physic-chemical parameters, e.g., organic carbon, total nitrogen and sulfate contents in sediments. During spring, the dominant species were assigned to the phylum Proteobacteria. Important opportunistic species was assigned to Acidobacteria, Actinobacteria and Bacteroidetes. The key components were identified to be species of the genera Pseudoalteromonas, Colwellia and Sphingobium, indicating the involvement of sediment microbiota in the degradation of sedimentary organic carbon, particularly that of pelagic origin, e.g., phytoplankton detritus and bivalve pseudo-feces. During spring, the microbial community was statistically similar along the depth profiles and among the three sampled stations. Similar spatial distributions were obtained in the remaining seasons. By contrast, the dominant species assemblages varied significantly among seasons, with key genera being Thioprofundum and Nitrosopumilus during summer and autumn and Thioprofundum and Ilumatobacter during winter. Network analysis revealed a seasonal shift in benthic nitrogen and sulfur metabolism associated with these variations in microbial community composition. Overall, our findings suggested that macro elements derived from pelagic inputs, particularly detrital phytoplankton, shaped the microbial community compositions at the seepage face, resulting in significant seasonal variations, while the influence of terrestrial materials transported by groundwater on the sediment microbiota at the seepage face found to be minor.
- subterranean estuary,
- microbiota,
- seepage face,
- seasonal variation,
- substrate

These authors contributed equally to this work.

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References(50)

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