Volume 39 Issue 10
Oct.  2020
Turn off MathJax
Article Contents
Juanjuan Yang, Dachun Yu, Songdong Shen. Expression analyses of miRNA Up-MIR-843 and its target genes in Ulva prolifera[J]. Acta Oceanologica Sinica, 2020, 39(10): 27-34. doi: 10.1007/s13131-020-1657-2
Citation: Juanjuan Yang, Dachun Yu, Songdong Shen. Expression analyses of miRNA Up-MIR-843 and its target genes in Ulva prolifera[J]. Acta Oceanologica Sinica, 2020, 39(10): 27-34. doi: 10.1007/s13131-020-1657-2

Expression analyses of miRNA Up-MIR-843 and its target genes in Ulva prolifera

doi: 10.1007/s13131-020-1657-2
Funds:  The National Key R&D Program of China under contract No. 2016YFC1402102; the project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
More Information
  • Corresponding author: E-mail: shensongdong@suda.edu.cn
  • Received Date: 2019-09-11
  • Accepted Date: 2019-11-22
  • Available Online: 2020-12-28
  • Publish Date: 2020-10-25
  • microRNAs (miRNA) families play a critical role in plant growth, development, and responses to abiotic stress. In this study, we characterized Up-miR-843 and its targets genes in Ulva prolifera responses to nitrogen depravation and heat stress. The data demonstrated that 184 target genes of Up-miR-843 could be successfully validated. N deficiency not heat stress stimulus induced increase in abundance of the Up-miR-843 while exhibited reverse expression of target genes, including cyclin A3 and cyclin L, which were strictly required for cell cycle progression. In addition, U. prolifera with highly expression of Up-miR-843 showed improved biomass, and photosynthesis compared with that under normal growth conditions. Thus, the N deprivation and heat responsive miRNAs might be a possible member mediating the expression of these target genes, which further regulated the growth of U. prolifera.
  • loading
  • [1]
    Arnon D I. 1949. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology, 24(1): 1–15. doi: 10.1104/pp.24.1.1
    [2]
    Bi Y M, Wang R L, Zhu T, et al. 2007. Global transcription profiling reveals differential responses to chronic nitrogen stress and putative nitrogen regulatory components in Arabidopsis. BMC Genomics, 8: 281. doi: 10.1186/1471-2164-8-281
    [3]
    Breuer G, Lamers P P, Martens D E, et al. 2012. The impact of nitrogen starvation on the dynamics of triacylglycerol accumulation in nine microalgae strains. Bioresource Technology, 124: 217–226. doi: 10.1016/j.biortech.2012.08.003
    [4]
    Chiou T J, Aung K, Lin S I, et al. 2006. Regulation of phosphate homeostasis by microRNA in Arabidopsis. The Plant Cell, 18(2): 412–421. doi: 10.1105/tpc.105.038943
    [5]
    Conley D J, Paerl H W, Howarth R W, et al. 2009. Controlling eutrophication: nitrogen and phosphorus. Science, 323(5917): 1014–1015. doi: 10.1126/science.1167755
    [6]
    Coutinho R, Zingmark R. 1993. Interactions of light and nitrogen on photosynthesis and growth of the marine macroalga Ulva curvata (Kützing) De Toni. Journal of Experimental Marine Biology and Ecology, 167(1): 11–19. doi: 10.1016/0022-0981(93)90180-V
    [7]
    Ferreira P, Hemerly A, De Almeida Engler J, et al. 1994. Three discrete classes of Arabidopsis cyclins are expressed during different intervals of the cell cycle. Proceedings of the National Academy of Sciences of the United States of America, 91(24): 11313–11317. doi: 10.1073/pnas.91.24.11313
    [8]
    Forment J, Naranjo M Á, Roldán M, et al. 2002. Expression of Arabidopsis SR-like splicing proteins confers salt tolerance to yeast and transgenic plants. The Plant Journal, 30(5): 511–519. doi: 10.1046/j.1365-313X.2002.01311.x
    [9]
    Gao Si, Guo Chengjin, Zhang Yongsheng, et al. 2016. Wheat microRNA member TaMIR444a is nitrogen deprivation-responsive and involves plant adaptation to the nitrogen-starvation stress. Plant Molecular Biology Reporter, 34(5): 931–946. doi: 10.1007/s11105-016-0973-3
    [10]
    Giacomelli J I, Weigel D, Chan R L, et al. 2012. Role of recently evolved miRNA regulation of sunflower HaWRKY6 in response to temperature damage. New Phytologist, 195(4): 766–773. doi: 10.1111/j.1469-8137.2012.04259.x
    [11]
    Hackenberg M, Gustafson P, Langridge P, et al. 2015. Differential expression of microRNAs and other small RNAs in barley between water and drought conditions. Plant Biotechnology Journal, 13(1): 2–13. doi: 10.1111/pbi.12220
    [12]
    Hasanuzzaman M, Nahar K, Alam M M, et al. 2013. Physiological, biochemical, and molecular mechanisms of heat stress tolerance in plants. International Journal of Molecular Sciences, 14(5): 9643–9684. doi: 10.3390/ijms14059643
    [13]
    He Yuan, Ma Yafeng, Du Yu, et al. 2018. Differential gene expression for carotenoid biosynthesis in a green alga Ulva prolifera based on transcriptome analysis. BMC Genomics, 19: 916. doi: 10.1186/s12864-018-5337-y
    [14]
    Hsieh L C, Lin S I, Shih A C C, et al. 2009. Uncovering small RNA-mediated responses to phosphate deficiency in Arabidopsis by deep sequencing. Plant Physiology, 151(4): 2120–2132. doi: 10.1104/pp.109.147280
    [15]
    Huang Aiyou, Wang Guangce, He Linwen, et al. 2011. Characterization of small RNAs from Ulva prolifera by high-throughput sequencing and bioinformatics analysis. Chinese Science Bulletin, 56(27): 2916–2921. doi: 10.1007/s11434-011-4678-6
    [16]
    Jones-Rhoades M W, Bartel D P, Bartel B. 2006. MicroRNAs and their regulatory roles in plants. Annual Review of Plant Biology, 57: 19–53. doi: 10.1146/annurev.arplant.57.032905.105218
    [17]
    Kouchi H, Sekine M, Hata S. 1995. Distinct classes of mitotic cyclins are differentially expressed in the soybean shoot apex during the cell cycle. The Plant Cell, 7(8): 1143–1155
    [18]
    Kruszka K, Pacak A, Swida-Barteczka A, et al. 2014. Transcriptionally and post-transcriptionally regulated microRNAs in heat stress response in barley. Journal of Experimental Botany, 65(20): 6123–6135. doi: 10.1093/jxb/eru353
    [19]
    Lartigue J, Neill A, Hayden B L, et al. 2003. The impact of salinity fluctuations on net oxygen production and inorganic nitrogen uptake by Ulva lactuca (Chlorophyceae). Aquatic Botany, 75(4): 339–350. doi: 10.1016/S0304-3770(02)00193-6
    [20]
    Li J, Wu L Q, Zheng W Y, et al. 2015. Genome-wide identification of microRNAs responsive to high temperature in rice (Oryza sativa) by high-throughput deep sequencing. Journal of Agronomy and Crop Science, 201(5): 379–388. doi: 10.1111/jac.12114
    [21]
    Liang Gang, Yang Fengxi, Yu Diqiu. 2010. MicroRNA395 mediates regulation of sulfate accumulation and allocation in Arabidopsis thaliana. The Plant Journal, 62(6): 1046–1057
    [22]
    Liao Jieren, Wu Xiayuan, Xing Zhiqiang, et al. 2017. γ-Aminobutyric Acid (GABA) accumulation in tea (Camellia sinensis L.) through the GABA shunt and polyamine degradation pathways under anoxia. Journal of Agricultural and Food Chemistry, 65(14): 3013–3018. doi: 10.1021/acs.jafc.7b00304
    [23]
    Lin Hanzhi, Jiang Peng, Zhang Jiaxu, et al. 2011. Genetic and marine cyclonic eddy analyses on the largest macroalgal bloom in the world. Environmental Science & Technology, 45(11): 5996–6002
    [24]
    Lu Yibin, Yang Lintong, Qi Yiping, et al. 2014. Identification of boron-deficiency-responsive microRNAs in Citrus sinensis roots by Illumina sequencing. BMC Plant Biology, 14: 123. doi: 10.1186/1471-2229-14-123
    [25]
    May P, Liao W, Wu Yijin, et al. 2013. The effects of carbon dioxide and temperature on microRNA expression in Arabidopsis development. Nature Communications, 4: 2145. doi: 10.1038/ncomms3145
    [26]
    McGlathery K J. 2001. Macroalgal blooms contribute to the decline of seagrass in nutrient-enriched coastal waters. Journal of Phycology, 37(4): 453–456. doi: 10.1046/j.1529-8817.2001.037004453.x
    [27]
    Meskiene I, Bögre L, Dahl M, et al. 1995. cycMs3, a novel B-type alfalfa cyclin gene, is induced in the G0-to-G1 transition of the cell cycle. The Plant Cell, 7(6): 759–771
    [28]
    Mironov V, De Veylder L, Van Montagu M, et al. 1999. Cyclin-dependent kinases and cell division in plants—the nexus. The Plant Cell, 11(4): 509–521
    [29]
    Niu Jun, Wang Jia, An Jiyong, et al. 2016. Integrated mRNA and miRNA transcriptome reveal a cross-talk between developing response and hormone signaling for the seed kernels of Siberian apricot. Scientific Reports, 6: 35675. doi: 10.1038/srep35675
    [30]
    Pérez-Mayorga D M, Ladah L B, Zertuche-González J A, et al. 2011. Nitrogen uptake and growth by the opportunistic macroalga Ulva lactuca (Linnaeus) during the internal tide. Journal of Experimental Marine Biology and Ecology, 406(1–2): 108–115
    [31]
    Patel D, Franklin K A. 2009. Temperature-regulation of plant architecture. Plant Signaling & Behavior, 4(7): 577–579
    [32]
    Paul S, Datta S K, Datta K. 2015. miRNA regulation of nutrient homeostasis in plants. Frontiers in Plant Science, 6: 232
    [33]
    Reichheld J P, Chaubet N, Shen Wenhui, et al. 1996. Multiple A-type cyclins express sequentially during the cell cycle in Nicotiana tabacum BY2 cells. Proceedings of the National Academy of Sciences of the United States of America, 93(24): 13819–13824. doi: 10.1073/pnas.93.24.13819
    [34]
    Stumpf R P, Gelfenbaum G, Pennock J R. 1993. Wind and tidal forcing of a buoyant plume, Mobile Bay, Alabama. Continental Shelf Research, 13(11): 1281–1301. doi: 10.1016/0278-4343(93)90053-Z
    [35]
    Suzuki N, Rivero R M, Shulaev V, et al. 2014. Abiotic and biotic stress combinations. New Phytologist, 203(1): 32–43. doi: 10.1111/nph.12797
    [36]
    Taylor R, Fletcher R L, Raven J A. 2001. Preliminary studies on the growth of selected ‘Green tide’ algae in laboratory culture: effects of irradiance, temperature, salinity and nutrients on growth rate. Botanica Marina, 44(4): 327–336
    [37]
    Vidal E A, Araus V, Lu Cheng, et al. 2010. Nitrate-responsive miR393/AFB3 regulatory module controls root system architecture in Arabidopsis thaliana. Proceedings of the National Academy of Sciences of the United States of America, 107(9): 4477–4482. doi: 10.1073/pnas.0909571107
    [38]
    Voinnet O. 2009. Origin, biogenesis, and activity of plant microRNAs. Cell, 136(4): 669–687. doi: 10.1016/j.cell.2009.01.046
    [39]
    Wang Zongling, Xiao Jie, Fan Shiliang, et al. 2015. Who made the world’s largest green tide in China?—an integrated study on the initiation and early development of the green tide in Yellow Sea Limnology and Oceanography, 60(4): 1105–1117. doi: 10.1002/lno.10083
    [40]
    Xu Zhenhua, Zhong Sihui, Li Xinhai, et al. 2011. Genome-wide identification of microRNAs in response to low nitrate availability in maize leaves and roots. PLoS One, 6(11): e28009. doi: 10.1371/journal.pone.0028009
    [41]
    Zhang Baohong. 2015. MicroRNA: a new target for improving plant tolerance to abiotic stress. Journal of Experimental Botany, 66(7): 1749–1761. doi: 10.1093/jxb/erv013
    [42]
    Zhao Meng, Ding Hong, Zhu Jiankang, et al. 2011. Involvement of miR169 in the nitrogen-starvation responses in Arabidopsis. New Phytologist, 190(4): 906–915. doi: 10.1111/j.1469-8137.2011.03647.x
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(3)  / Tables(4)

    Article Metrics

    Article views (289) PDF downloads(10) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return