Phenotypic plasticity and taxonomy of <i>Cladophora gracilis</i> (Griffiths) Kützing (Cladophorales, Chlorophyta) in the western Yellow Sea with the implication of its DNA barcode

Bingxin Huang; Linhong Teng; Jingjing Jiang; Lanping Ding

doi:10.1007/s13131-020-1586-0

Volume 39 Issue 10

Oct. 2020

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Bingxin Huang, Linhong Teng, Jingjing Jiang, Lanping Ding. Phenotypic plasticity and taxonomy of Cladophora gracilis (Griffiths) Kützing (Cladophorales, Chlorophyta) in the western Yellow Sea with the implication of its DNA barcode[J]. Acta Oceanologica Sinica, 2020, 39(10): 162-170. doi: 10.1007/s13131-020-1586-0

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Bingxin Huang, Linhong Teng, Jingjing Jiang, Lanping Ding. Phenotypic plasticity and taxonomy of Cladophora gracilis (Griffiths) Kützing (Cladophorales, Chlorophyta) in the western Yellow Sea with the implication of its DNA barcode[J]. Acta Oceanologica Sinica, 2020, 39(10): 162-170. doi: 10.1007/s13131-020-1586-0

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Phenotypic plasticity and taxonomy of Cladophora gracilis (Griffiths) Kützing (Cladophorales, Chlorophyta) in the western Yellow Sea with the implication of its DNA barcode

doi: 10.1007/s13131-020-1586-0

Bingxin Huang^{1, 2, 3},
Linhong Teng²,
Jingjing Jiang^{1, 3},
Lanping Ding^{1, 2, 3
,
,}

1.
Collage of Life Sciences, Tianjin Normal University, Tianjin 300387, China
2.
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
3.
Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin 300387, China

Funds: The National Natural Science Foundation of China under contract Nos 31670199, 31400186 and 30499340; the Knowledge Innovation Project of Chinese Academy of Sciences under contract No. KSCX2-YW -Z-018; the Scientific Research Plan of Tianjin Municipal Education Committee under contract No. JW1705; the Research Fund for Talented Scholars of Tianjin Normal University (2016).

More Information

Corresponding author: E-mail: skydlp@tjnu.edu.cn
Received Date: 2019-04-18
Accepted Date: 2019-09-20

Available Online: 2020-12-28

Publish Date: 2020-10-25

Abstract

Abstract

Due to extensive morphological plasticity, the taxonomy of Cladophora species remains unclear. As one of the widely distributed species, C. gracilis was reported to hold many varieties, which make it difficult to identify the species. This study explored the morphology diversity of nine C. gracilis samples collected along the coast of western Yellow Sea. Some samples showed extremely varied characteristics, by which one cannot classify them correctly. Hence, 18S rDNA and nuclear ribosomal DNA internal transcribed spacer regions (ITS) sequences were employed to delimit species. For 18S rDNA, sequence similarity ranged from 99.6% to 100%. For ITS region, the similarity ranged from 98.7% to 100%. Molecular data strongly suggested that the morphologically heterogeneous samples were actually the same species. Characteristics comparison of the samples revealed that the taxonomy criteria including branching pattern and density, thallus color, height and texture varied widely, influenced by environmental conditions and age of alga. Besides, cell dimensions, as the relatively stable criterion, also exhibited intraspecific variance. Successful application of 18S rDNA and ITS sequences indicated that molecular method can be a powerful assistant as DNA barcodes to traditional morphology taxonomy.
- Cladophora gracilis,
- morphology observation,
- molecular identification,
- 18S rDNA,
- ITS

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References(35)

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