Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass <i>Zostera marina</i>

Yan Wenjie; Liu Jiao; Seng Samphal; Zhou Bin; Ding Kuke

doi:10.1007/s13131-019-1429-z

Article Contents

Article Navigation > Acta Oceanologica Sinica > 2019 > 38(10): 111-115

Yan Wenjie, Liu Jiao, Seng Samphal, Zhou Bin, Ding Kuke. Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina[J]. Acta Oceanologica Sinica, 2019, 38(10): 111-115. doi: 10.1007/s13131-019-1429-z

Citation:

Yan Wenjie, Liu Jiao, Seng Samphal, Zhou Bin, Ding Kuke. Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina[J]. Acta Oceanologica Sinica, 2019, 38(10): 111-115. doi: 10.1007/s13131-019-1429-z

Citation:

PDF( 1475 KB)

Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina

doi: 10.1007/s13131-019-1429-z

1.
Department of Marine Ecology, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
2.
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
3.
Faculty of Fisheries & Aquaculture, Royal University of Agriculture, Phnom Penh 12101, Cambodia
4.
National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China;Key Laboratory of Radiological Protection and Nuclear Emergency, Chinese Center for Disease Control and Prevention, Beijing 100088, China

Received Date: 2018-05-20

Abstract

Abstract

The response of glutathione S-transferase (GST) in Zostera marina to temperature variation was analyzed at molecular level by cloning the microsomal GST gene and texting the microsomal GST expression regularity under different temperature. Specific speaking, express ZmGST in Escherichia coli, then purify the recombinant protein and make the thermal stability analysis. Therefore, the experiments were carried out to provide a theoretical basis for the further elaboration to the population degradation mechanisms of Z. marina. In conclusion, the thermostability and the response of ZmGST gene to temperature changes can determine its temperature tolerance range, and affect its resilience in turn.
- Zostera marina|antioxidant enzyme|glutathione S-transferase (GST)|temperature|enzyme activity

FullText(HTML)

References(19)

References

Costanza R, d’Arge R, De Groot R, et al. 1997. The value of the world’s ecosystem services and natural capital. Nature, 387(6630): 253–260, doi: 10.1038/387253a0

Den Hartog C. 1970. The Seagrasses of the World. Amsterdam: North Holland Publishing Company, 265

Dixon D P, Lapthorn A, Edwards R. 2002. Plant glutathione transferases. Genome Biology, 3(3): eviews3004.1

Edwards R, Dixon D P, Walbot V. 2000. Plant glutathione S-transferases: enzymes with multiple functions in sickness and in health. Trends in Plant Science, 5(5): 193–198, doi: 10.1016/S1360-1385(00)01601-0

Fourqurean J W, Duarte C M, Kennedy H, et al. 2012. Seagrass ecosystems as a globally significant carbon stock. Nature Geoscience, 5(7): 505–509, doi: 10.1038/ngeo1477

Green E P, Short F T. 2003. World Atlas of Seagrasses. Berkeley: University of California Press, 185–192

Hemminga M A, Duarte C M. 2000. Seagrass Ecology. Cambridge: Cambridge University Press, 298

Larkum A W D, Orth R J, Duarte C M. 2006. Seagrasses: Biology, Ecology and Conservation. Netherlands: Springer, 25–50

Les D H, Cleland M A, Waycott M. 1997. Phylogenetic studies in Alismatidae, Ⅱ: evolution of marine angiosperms (seagrasses) and hydrophily. Systematic Botany, 22(3): 443–463, doi: 10.2307/2419820

Logue J, Tiku P, Cossins A R. 1995. Heat injury and resistance adaptation in fish. Journal of Thermal Biology, 20(1–2): 191–197

Mannervik B, Danielson U H, Ketterer B. 1988. Glutathione transferase-structure and catalytic activity. Critical Reviews in Biochemistry, 23(2): 283–337

Martin J R, Specht J E, Williams J H, et al. 1978. Temperature tolerance in soybeans: I. Evaluation of a technique for assessing cellular membrane thermostability. Crop Science, 19(1): 75–81

Olsen J L, Rouzé P, Verhelst B, et al. 2016. The genome of the seagrass Zostera marina reveals angiosperm adaptation to the sea. Nature, 530(7590): 331–335, doi: 10.1038/nature16548

Rushmore T H, Pickett C B. 1993. Glutathione S-transferases, structure, regulation, and therapeutic implications. The Journal of Biological Chemistry, 268(16): 11475–11478

Sheehan D, Meade G, Foley V M, et al. 2001. Structure, function and evolution of glutathione transferases: implications for classification of non-mammalian members of an ancient enzyme superfamily. Biochemical Journal, 360(1): 1–16, doi: 10.1042/bj3600001

Wilce M C J, Parker M W. 1994. Structure and function of glutathione S-transferases. Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular Enzymology, 1205(1): 1–18, doi: 10.1016/0167-4838(94)90086-8

Wright J P, Jones C G. 2006. The concept of organisms as ecosystem engineers ten years on: progress, limitations, and challenges. BioScience, 56(3): 203–209, doi: 10.1641/0006-3568(2006)056[0203:TCOOAE]2.0.CO;2

Yan Fei, Yang Wenkui, Li Xinyang, et al. 2008. A trifunctional enzyme with glutathione S-transferase, glutathione peroxidase and superoxide dismutase activity. Biochimica et Biophysica Acta (BBA)-General Subjects, 1780(6): 869–872, doi: 10.1016/j.bbagen.2008.03.003

Zhang Yi, Lu Tiegang. 2011. The research of Reactive Oxygen Species (ROS) in plants. Current Biotechnology (in Chinese), 1(4): 242–248

Relative Articles

Supplements(0)

Cited By

Proportional views