Identification and characterization of a delta-12 fatty acid desaturase gene from marine microalgae <i>Isochrysis galbana</i>

Han Xiaotian; Wang Shuai; Zheng Li; Liu Wanshun

doi:10.1007/s13131-019-1354-1

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Article Navigation > Acta Oceanologica Sinica > 2019 > 38(2): 107-113

Han Xiaotian, Wang Shuai, Zheng Li, Liu Wanshun. Identification and characterization of a delta-12 fatty acid desaturase gene from marine microalgae Isochrysis galbana[J]. Acta Oceanologica Sinica, 2019, 38(2): 107-113. doi: 10.1007/s13131-019-1354-1

Citation:

Han Xiaotian, Wang Shuai, Zheng Li, Liu Wanshun. Identification and characterization of a delta-12 fatty acid desaturase gene from marine microalgae Isochrysis galbana[J]. Acta Oceanologica Sinica, 2019, 38(2): 107-113. doi: 10.1007/s13131-019-1354-1

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Identification and characterization of a delta-12 fatty acid desaturase gene from marine microalgae Isochrysis galbana

doi: 10.1007/s13131-019-1354-1

1.
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China;Changjiang Estuary Marine Ecosystem Research Station, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Marine Ecology and Environmental Science Laboratory, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China
2.
Key Laboratory for Marine Bioactive Substances and Modern Analytical Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China
3.
Marine Ecology and Environmental Science Laboratory, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China;Key Laboratory for Marine Bioactive Substances and Modern Analytical Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China
4.
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China

Received Date: 2017-11-23

Abstract

Abstract

The cDNA of the delta-12 fatty acid desaturase gene, IgFAD2, was cloned from the marine microalgae Isochrysis galbana, a species capable of producing docosahexaenoic acid. Sequence analysis indicated that the open reading frame measured a length of 1 158 bp and encoded 386 amino acids with a predicted molecular weight of 42.8 kDa and an isoelectric point of 9.2. Computational analysis of the protein sequence of IgFAD2 showed typical features of membrane-bound desaturase such as three conserved histidine boxes along with four membrane-spanning regions that were universally present among plant desaturases. Quantitative real-time PCR results showed that the abundance of IgFAD2 transcript was significantly upregulated under different environmental stresses including low temperature (15℃), high salinity (salinity of 62 and 93), and nitrogen starvation (220 μmol/L). Heterologous expression indicated that yeast cells transformed with a plasmid construct containing IgFAD2 could convert endogenous oleic acid (18:1^Δ9, OA) into linoleic acid (18:2^{Δ9, 12}, LA). These findings confirm that I. galbana IgFAD2 plays important roles in the biosynthetic pathways of unsaturated fatty acids.
- delta-12 fatty acid desaturase,
- expression analysis,
- Isochrysis galbana

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References(40)

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