Cloning and characterization of a new endo-type polyG-specific alginate lyase from bacteria <i>Vibrio</i> sp. QD-5

Yaxi Chao; Shuyan Wang; Suqi Wu; Jiaqiang Wei; Hao Chen

doi:10.1007/s13131-018-1226-0

Volume 38 Issue 6

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Article Navigation > Acta Oceanologica Sinica > 2019 > 38(6): 65-74

Yaxi Chao, Shuyan Wang, Suqi Wu, Jiaqiang Wei, Hao Chen. Cloning and characterization of a new endo-type polyG-specific alginate lyase from bacteria Vibrio sp. QD-5[J]. Acta Oceanologica Sinica, 2019, 38(6): 65-74. doi: 10.1007/s13131-018-1226-0

Citation:

Yaxi Chao, Shuyan Wang, Suqi Wu, Jiaqiang Wei, Hao Chen. Cloning and characterization of a new endo-type polyG-specific alginate lyase from bacteria Vibrio sp. QD-5[J]. Acta Oceanologica Sinica, 2019, 38(6): 65-74. doi: 10.1007/s13131-018-1226-0

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Cloning and characterization of a new endo-type polyG-specific alginate lyase from bacteria Vibrio sp. QD-5

doi: 10.1007/s13131-018-1226-0

Yaxi Chao¹,
Shuyan Wang¹,
Suqi Wu^{1, 2},
Jiaqiang Wei¹,
Hao Chen^{1, 2
,
,}

1.
Key Laboratory of Marine Bioactive Substances, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China
2.
Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China

Funds: The Marine Public Welfare Project of SOA under contract No. 201505032; the Scientific and Technological Innovation Project financially of Qingdao National Laboratory for Marine Science and Technology under contract No. 2016ASKJ14.

More Information

Corresponding author: E-mail: hchen@fio.org.cn
Received Date: 2018-02-02
Accepted Date: 2018-03-22

Available Online: 2020-04-21

Publish Date: 2019-06-01

Abstract

Abstract

Seven bacterial clones with alginate-utilizing activity were isolated from rotten kelp. By activity test, the Vibrio sp. QD-5 with the potential alginate-degrading capability was chosen to carry out the draft genome sequencing, and the result showed that the Vibrio sp. QD-5 containing an alginate lyase gene cluster. One of these genes, aly-IV, was cloned and characterized for the first time. After overexpression, Aly-IV, with a molecular mass of about 62 kDa and a theoretical isoelectric point (pI) of 5.12, was purified to a specific activity of 1 256.78 U/mg and showed highest activity at 35°C in the Tris-HCl buffer at pH of 8.9. Moreover, the enzyme activity was enhanced by the metal ions of Na⁺, K⁺ and Mg²⁺ under certain concentration. Aly-IV degraded favorably polyG blocks in an endo-type, yielding monomer and dimer as the main products. Due to its high substrate specificity, Aly-IV could be used as a potential tool for production of polyG oligosaccharides with low degree of polymerization (DP) and for determining the fine structure of alginate.
- alginate lyase,
- Vibrio sp.,
- alginate,
- poly-α-L-guluronic acid lyase,
- oligosaccharides

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References(32)

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