QIAO Ying, WANG Jun, MAO Yong, LIU Min, SONG Xiaohong, SU Yongquan, WANG Chunzhong, ZHENG Zhipeng. Identification and molecular characterization of Cathepsin L gene and its expression analysis during early ontogenetic development of kuruma shrimp Marsupenaeus japonicus[J]. Acta Oceanologica Sinica, 2017, 36(6): 52-60. doi: 10.1007/s13131-017-0983-5
Citation: QIAO Ying, WANG Jun, MAO Yong, LIU Min, SONG Xiaohong, SU Yongquan, WANG Chunzhong, ZHENG Zhipeng. Identification and molecular characterization of Cathepsin L gene and its expression analysis during early ontogenetic development of kuruma shrimp Marsupenaeus japonicus[J]. Acta Oceanologica Sinica, 2017, 36(6): 52-60. doi: 10.1007/s13131-017-0983-5

Identification and molecular characterization of Cathepsin L gene and its expression analysis during early ontogenetic development of kuruma shrimp Marsupenaeus japonicus

doi: 10.1007/s13131-017-0983-5
  • Received Date: 2016-02-16
  • Rev Recd Date: 2016-03-29
  • Cathepsin L gene is a member of the cysteine proteinase gene group. In this study Cathepsin L gene was isolated from Kuruma shrimp Marsupenaeus japonicus (Mj-Cathepsin L) and the full-length DNA sequence was 1 963 bp. Mj-Cathepsin L protein showed high homologies with other Cathepsin L proteins documented in vertebrates, mollusks and other crustaceans. Expression analysis of Mj-Cathepsin L gene in different tissues revealed that it was predominant in hepatopancreas. During early ontogenetic development stages Mj-Cathepsin L showed a development-regulated expression, and the Mj-Cathepsin L showed a molting stage-regulated expression during the five molting stages, inferring its role in the ontogenic development of M. japonicus. Two kinds of forms of Mj-Cathepsin L protein:pro-Cathepsin L and Cathepsin L were measured in hepatopancreas, stomach and intestine by Western Blotting.
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