Characterization of uncultivated magnetotactic bacteria from the sediments of Yuehu Lake, China

DU Haijian; ZHANG Rui; ZHANG Wenyan; XU Cong; CHEN Yiran; PAN Hongmiao; ZHOU Ke; WU Long-fei; XIAO Tian

doi:10.1007/s13131-017-0980-8

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Article Navigation > Acta Oceanologica Sinica > 2017 > 36(2): 94-104

DU Haijian, ZHANG Rui, ZHANG Wenyan, XU Cong, CHEN Yiran, PAN Hongmiao, ZHOU Ke, WU Long-fei, XIAO Tian. Characterization of uncultivated magnetotactic bacteria from the sediments of Yuehu Lake, China[J]. Acta Oceanologica Sinica, 2017, 36(2): 94-104. doi: 10.1007/s13131-017-0980-8

Citation:

DU Haijian, ZHANG Rui, ZHANG Wenyan, XU Cong, CHEN Yiran, PAN Hongmiao, ZHOU Ke, WU Long-fei, XIAO Tian. Characterization of uncultivated magnetotactic bacteria from the sediments of Yuehu Lake, China[J]. Acta Oceanologica Sinica, 2017, 36(2): 94-104. doi: 10.1007/s13131-017-0980-8

Citation:

DU Haijian, ZHANG Rui, ZHANG Wenyan, XU Cong, CHEN Yiran, PAN Hongmiao, ZHOU Ke, WU Long-fei, XIAO Tian. Characterization of uncultivated magnetotactic bacteria from the sediments of Yuehu Lake, China[J]. Acta Oceanologica Sinica, 2017, 36(2): 94-104. doi: 10.1007/s13131-017-0980-8

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Characterization of uncultivated magnetotactic bacteria from the sediments of Yuehu Lake, China

doi: 10.1007/s13131-017-0980-8

1.
Key Laboratory for Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China
2.
Key Laboratory for Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China;University of Chinese Academy of Sciences, Beijing 100049, China
3.
College of Resources and Environment, Qingdao Agriculture University, Qingdao 266109, China
4.
Aix Marseille Université, CNRS, LCB UMR 7257, Institut de Microbiologie de la Méditerranée, Marseille F-13402, France

Received Date: 2015-11-04
Rev Recd Date: 2016-08-23

Abstract

Abstract

Marine magnetotactic bacteria were collected from the intertidal sediments of Yuehu Lake (China), where their abundance reached 10³-10⁴ ind./cm³. Diverse morphotypes of magnetotactic bacteria were observed, including cocci and oval, vibrio-, spirillum-, rod-, elliptical-, handle- and bar-shaped forms. The magnetococci were the most abundant, and had flagella arranged in parallel within a bundle. The majority of magnetosomes were arranged in one, two or multiple chains, although irregular arrangements were also evident. All the results of high-resolution transmission electron microscopy (HRTEM) analysis show that magnetosome crystals were composed of Fe₃O₄, and their morphology was specific to particular cell morphotypes. By the 16S rRNA gene sequence analysis, we found fourteen operational taxonomic units (OTUs) which were related to magnetotactic bacteria. Among these, thirteen belonged to the Alphaproteobacteria and one to the Gammaproteobacteria. Compared with known axenic and uncultured marine magnetotactic bacteria, the 16S rRNA gene sequences of most magnetotactic bacteria collected from the Yuehu Lake exhibited sequence identities ranging from 90.1% to 96.2% (<97%). The results indicate that microbial communities containing previously unidentified magnetotactic bacteria occur in the Yuehu Lake.
- magnetotactic bacteria,
- magnetosome,
- biodiversity,
- Yuehu Lake,
- intertidal sediments

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References(73)

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