Superoxide dismutase genes in <i>Pyropia haitanensis</i>: molecular cloning, characterization and mRNA expression

JI Dehua; XU Yan; XIAO Haidong; CHEN Changsheng; XU Kai; XIE Chaotian

doi:10.1007/s13131-016-0873-2

Volume 35 Issue 6

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Article Navigation > Acta Oceanologica Sinica > 2016 > 35(6): 101-111

JI Dehua, XU Yan, XIAO Haidong, CHEN Changsheng, XU Kai, XIE Chaotian. Superoxide dismutase genes in Pyropia haitanensis: molecular cloning, characterization and mRNA expression[J]. Acta Oceanologica Sinica, 2016, 35(6): 101-111. doi: 10.1007/s13131-016-0873-2

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JI Dehua, XU Yan, XIAO Haidong, CHEN Changsheng, XU Kai, XIE Chaotian. Superoxide dismutase genes in Pyropia haitanensis: molecular cloning, characterization and mRNA expression[J]. Acta Oceanologica Sinica, 2016, 35(6): 101-111. doi: 10.1007/s13131-016-0873-2

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Superoxide dismutase genes in Pyropia haitanensis: molecular cloning, characterization and mRNA expression

doi: 10.1007/s13131-016-0873-2

Fisheries College, Jimei University, Xiamen 361021, China

Received Date: 2015-03-12
Rev Recd Date: 2015-08-07

Abstract

Abstract

Superoxide dismutase (SOD, EC 1.15.1.1) is the first and most important line of cellular defense against oxidative stress. In this study, based on unigene sequences of P. haitanensis, three full-length PhSOD genes were obtained by RACE technology, and named PhMSD, PhCSD1 and PhCSD2. The full-length cDNAs of these genes comprised 973, 1 029 and 954 nucleotides, respectively. The cDNAs encoded proteins of 224, 134 and 216 amino acids, with isoelectric points of 5.75, 4.65 and 10.74, respectively. Based on their conserved motifs and phylogenetic tree analysis, the three PhSODs were divided into two SOD types: PhMSD is a Mn-SOD, and PhCSD1 and PhCSD2 are CuZn-SODs. qPCR was used to measure the expression of the three PhSOD genes in different life phases of P. haitanensis, and under different periods of high-temperature stress and different levels of desiccation. In the different life phases of P. haitanensis, the expression levels of the three PhSODs were all signi.cantly higher in the thallus than in the conchocelis. During high-temperature and desiccation stress, the expression levels of PhCSD1 and PhCSD2 were highly induced by the O₂^- content, but the expression level of PhMSD was highly depressed by high temperature stress and showed no significant change during desiccation.
- superoxide dismutase,
- gene cloning,
- desiccation stress,
- high-temperature stress,
- qPCR

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