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Volume 35 Issue 6
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Article Navigation >  Acta Oceanologica Sinica  > 2016  >  35(6): 101-111
JI Dehua, XU Yan, XIAO Haidong, CHEN Changsheng, XU Kai, XIE Chaotian. Superoxide dismutase genes in Pyropia haitanensis: molecular cloning, characterization and mRNA expression[J]. Acta Oceanologica Sinica, 2016, 35(6): 101-111. doi: 10.1007/s13131-016-0873-2
Citation: JI Dehua, XU Yan, XIAO Haidong, CHEN Changsheng, XU Kai, XIE Chaotian. Superoxide dismutase genes in Pyropia haitanensis: molecular cloning, characterization and mRNA expression[J]. Acta Oceanologica Sinica, 2016, 35(6): 101-111. doi: 10.1007/s13131-016-0873-2
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Superoxide dismutase genes in Pyropia haitanensis: molecular cloning, characterization and mRNA expression

doi: 10.1007/s13131-016-0873-2

  • Fisheries College, Jimei University, Xiamen 361021, China

  • Received Date: 2015-03-12
  • Rev Recd Date: 2015-08-07
    Abstract
  • Superoxide dismutase (SOD, EC 1.15.1.1) is the first and most important line of cellular defense against oxidative stress. In this study, based on unigene sequences of P. haitanensis, three full-length PhSOD genes were obtained by RACE technology, and named PhMSD, PhCSD1 and PhCSD2. The full-length cDNAs of these genes comprised 973, 1 029 and 954 nucleotides, respectively. The cDNAs encoded proteins of 224, 134 and 216 amino acids, with isoelectric points of 5.75, 4.65 and 10.74, respectively. Based on their conserved motifs and phylogenetic tree analysis, the three PhSODs were divided into two SOD types: PhMSD is a Mn-SOD, and PhCSD1 and PhCSD2 are CuZn-SODs. qPCR was used to measure the expression of the three PhSOD genes in different life phases of P. haitanensis, and under different periods of high-temperature stress and different levels of desiccation. In the different life phases of P. haitanensis, the expression levels of the three PhSODs were all signi.cantly higher in the thallus than in the conchocelis. During high-temperature and desiccation stress, the expression levels of PhCSD1 and PhCSD2 were highly induced by the O2- content, but the expression level of PhMSD was highly depressed by high temperature stress and showed no significant change during desiccation.
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  • Alscher R G, Erturk N, Heath L S. 2002. Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. Journal of Experimental Botany, 53(372): 1331-1341
    Armbrust E E, Berges J A, Bowler C, et al. 2004. The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and metabolism. Science, 306(5693): 79-86
    Blouin N A, Brodie J A, Grossman A C, et al. 2011. Porphyra: a marine crop shaped by stress. Trends in Plant Science, 16(1): 29-37
    Burritt D J, Larkindale J, Hurd C L. 2002. Antioxidant metabolism in the intertidal red seaweed Stictosiphonia arbuscula following desiccation. Planta, 215(5): 829-838
    Chen Changsheng, Ji Dehua, Xie Chaotian, et al. 2008. Preliminary study on selecting the high temperature resistance strains and economic traits of Porphyra haitanensis. Haiyang Xuebao (in Chinese), 30(5): 100-106
    Contreras-Porcia L, Thomas D, Flores V, et al. 2011. Tolerance to ox-idative stress induced by desiccation in Porphyra columbina (Bangiales, Rhodophyta). Journal of Experimental Botany, 62(6): 1815-1829
    Cirulis J T, Scott J A, Ross G M. 2013. Management of oxidative stress by microalgae. Canadian Journal of Physiology and Pharmaco-logy, 91(1): 15-21
    Davison I R, Pearson G A. 1996. Stress tolerance in intertidal sea-weeds. Journal of Phycology, 32(2): 197-211
    Fink R C, Scandalios J G. 2002. Molecular evolution and structure-function relationships of the superoxide dismutase gene famil-ies in angiosperms and their relationship to other eukaryotic and prokaryotic superoxide dismutases. Archives of Biochem-istry and Biophysics, 399(1): 19-36
    Fridovich I. 1995. Superoxide radical and superoxide dismutases. An-nual Review of Biochemistry, 64(1): 97-112
    Kliebenstein D J, Monde R A, Last R L. 1998. Superoxide dismutase in Arabidopsis: an eclectic enzyme family with disparate regula-tion and protein localization. Plant Physiology, 118(2): 637-650
    Kumar M, Gupta V, Trivedi N, et al. 2011. Desiccation induced oxid-ative stress and its biochemical responses in intertidal red alga Gracilaria corticata (Gracilariales, Rhodophyta). Environment-al and Experimental Botany, 72(2): 194-201
    Liu Yenchun. 2009. Mechanism for differential desiccation tolerance in porphyra species[dissertation]. Boston: Northeastern Uni-versity
    Lu Ning, Zang Xiaonan, Zhang Xuecheng, et al. 2012. Gene cloning, expression and activity analysis of manganese superoxide dis-mutase from two strains of Gracilaria lemaneiformis (Gracilari-aceae, Rhodophyta) under Heat Stress. Molecules, 17(4): 4522-4532
    Matsuzaki M, Misumi O, Shin-I T, et al. 2004. Genome sequence of the ultrasmall unicellular red alga Cyanidioschyzon merolae 10D. Nature, 428(6983): 653-657
    Mittler R, Vanderauwera S, Gollery M, et al. 2004. Reactive oxygen gene network of plants. Trends in Plant Science, 9(10): 490-498
    Sahoo D, Tang Xiaorong, Yarish C. 2002. Porphyra-the economic sea-weed as a new experimental system. Current Science, 83(11): 1313-1316
    Scandalios J G. 1997. Molecular genetics of superoxide dismutase in plants. In: Scandalios J G, ed. Oxidative Stress and the Molecu-lar Biology of Antioxidant Defenses. New York: Cold Spring Harbor Laboratory Press
    Tamura K, Peterson D, Peterson N, et al. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evol-utionary distance, and maximum parsimony methods. Molecu-lar Biology and Evolution, 28(10): 2731-2739
    Tomanek L. 2011. Environmental proteomics: changes in the proteo-me of marine organisms in response to environmental stress, pollutants, infection, symbiosis and development. Annual Re-view of Marine Science, 3(3): 373-399
    Wang Feng, Wang Hongbin, Liu Bing, et al. 2006a. Cloning and char-acterization of a novel splicing isoform of the iron-superoxide dismutase gene in rice (Oryza sativa L.). Plant Cell Reports, 24(12): 734-742
    Wang Rong, Liu Tao, Zhou Xiaojun, et al. 2006b. Cloning and se-quence analysing of Mn-SOD Gene from Porphyra yezoensis Udea. Chinese High Technology Letters (in Chinese), 16(5): 522-528
    Xie Chaotian, Li Bing, Xu Yan, et al. 2013. Characterization of the global transcriptome for Pyropia haitanensis (Bangiales, Rhodophyta) and development of cSSR markers. BMC Genom-ics, 14(1): 107
    Xie Jia, Xu Yan, Ji Dehua, et al. 2014. Physiological response of the an-tioxidant system in Pyropia haitanensis to desiccation stress. Journal of Fishery Sciences of China (in Chinese), 21(2): 405-412
    Xu Yan, Chen Changsheng, Ji Dehua, et al. 2014. Proteomic profile analysis of Pyropia haitanensis in response to high-temperat-ure stress. Journal of Applied Phycology, 26(1): 607-618
    Yan Xinghong, Lv Feng, Liu Changjun, et al. 2010. Selection and char-acterization of a high-temperature tolerant strain of Porphyra haitanensis Chang et Zheng (Bangiales, Rhodophyta). Journal of Applied Phycology, 22(4): 511-516
    Zhang Xuecheng, Qin Song, Ma Jiahai, et al. 2005. The Genetics of Marine Algae (in Chinese). Beijing: China Agriculture Press, 184-203
    Zhang Yuan, Xie Chaotian, Chen Changsheng, et al. 2011. Physiolo-gical responses of gametophytic blades of Porphyra haitanen-sis to rising temperature stresses. Journal of Fisheries of China (in Chinese), 35(3): 379-386
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