Morphological and molecular discrimination of green macroalgae <i>Chaetomorpha aerea</i> and <i>C. linum</i>

HUANG Bingxin; TENG Linhong; DING Lanping

doi:10.1007/s13131-016-0841-x

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Article Navigation > Acta Oceanologica Sinica > 2016 > 35(4): 118-123

HUANG Bingxin, TENG Linhong, DING Lanping. Morphological and molecular discrimination of green macroalgae Chaetomorpha aerea and C. linum[J]. Acta Oceanologica Sinica, 2016, 35(4): 118-123. doi: 10.1007/s13131-016-0841-x

Citation:

HUANG Bingxin, TENG Linhong, DING Lanping. Morphological and molecular discrimination of green macroalgae Chaetomorpha aerea and C. linum[J]. Acta Oceanologica Sinica, 2016, 35(4): 118-123. doi: 10.1007/s13131-016-0841-x

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Morphological and molecular discrimination of green macroalgae Chaetomorpha aerea and C. linum

doi: 10.1007/s13131-016-0841-x

1.
Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin 300387, China;Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Marine Biology Institute, Shantou University, Shantou 515063, China
2.
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Graduate University of Chinese Academy of Sciences, Beijing 100049, China

Received Date: 2015-01-13
Rev Recd Date: 2015-08-07

Abstract

Abstract

Green macroalgae Chaetomorpha aerea and C. linum are taxonomically confused. In this paper, we tried morphological and molecular analyses to separate these two species. C. aerea and C. linum can be distinguished from morphological characteritics, such as frond dimension, cells size and shape, their mean length/width ratios(LWR), and cell walls constriction. Thalli of C. aerea attenuate basipetally, with diameter 270-500μm at upper portion, 160-360μm at middle portion, 100-160μm at basal portion. For the upper part, the length of cells is less than their diameter. Cell walls usually constrict at the dissepiments, which are pellucid or colorless and give the filament beaded appearance. In contrast, thalli of C. linum often have a constant diameter of 90-300μm within the same individual, cell walls usually do not constrict and cells are cylindrical or barrel shaped. The LWR is larger than that of C. aerea. Results show that the pairwise distance between two species is 3.6%-3.7% for 18S rRNA gene and 53.5%-54.3% for ITS region. In phylogeny, they distribute at distant clades, which confirms a genetic divergence at molecular level. In addition, morphological data indicates that filament diameter of C. linum samples is highly variable, ranging from 90μm to 300μm. Then these two species can be considered as separate species.
- Chaetomorpha aerea,
- Chaetomorpha linum,
- molecular identification,
- morphology comparison,
- 18S rRNA,
- ITS

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References(39)

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