Phylogenetic analysis and biological characteristic tests of marine bacteria isolated from Southern Ocean (Indian sector) water

GUPTA Pratibha; BALAJI Raju; PARANI M; CHANDRA T S; SHUKLA P; KUMAR Anil; BANDOPADHYAY Rajib

doi:10.1007/s13131-015-0709-5

Article Contents

Article Navigation > Acta Oceanologica Sinica > 2015 > 34(8): 73-82

GUPTA Pratibha, BALAJI Raju, PARANI M, CHANDRA T S, SHUKLA P, KUMAR Anil, BANDOPADHYAY Rajib. Phylogenetic analysis and biological characteristic tests of marine bacteria isolated from Southern Ocean (Indian sector) water[J]. Acta Oceanologica Sinica, 2015, 34(8): 73-82. doi: 10.1007/s13131-015-0709-5

Citation:

GUPTA Pratibha, BALAJI Raju, PARANI M, CHANDRA T S, SHUKLA P, KUMAR Anil, BANDOPADHYAY Rajib. Phylogenetic analysis and biological characteristic tests of marine bacteria isolated from Southern Ocean (Indian sector) water[J]. Acta Oceanologica Sinica, 2015, 34(8): 73-82. doi: 10.1007/s13131-015-0709-5

Citation:

GUPTA Pratibha, BALAJI Raju, PARANI M, CHANDRA T S, SHUKLA P, KUMAR Anil, BANDOPADHYAY Rajib. Phylogenetic analysis and biological characteristic tests of marine bacteria isolated from Southern Ocean (Indian sector) water[J]. Acta Oceanologica Sinica, 2015, 34(8): 73-82. doi: 10.1007/s13131-015-0709-5

PDF( 18152 KB)

Phylogenetic analysis and biological characteristic tests of marine bacteria isolated from Southern Ocean (Indian sector) water

doi: 10.1007/s13131-015-0709-5

1.
Department of Bio-Engineering, Birla Institute of Technology, Mesra, Ranchi 835215, Jharkhand, India
2.
Department of Genetic Engineering, Kattankulathur Campus, SRM University, Chennai 600033, Tamilnadu, India
3.
Microbiology and Genetics Laboratory, Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600036, Tamilnadu, India
4.
Department of Bio-Engineering, Birla Institute of Technology, Mesra, Ranchi 835215, Jharkhand, India;Department of Microbiology, Maharshi Dayanand University, Rohtak 124001, Haryana, India
5.
OSSG Department, National Centre for Antarctic and Ocean Research, Ministry of Earth Sciences, Government of India, Headland Sada, Vasco-da-Gama, Goa 403804, India
6.
Department of Bio-Engineering, Birla Institute of Technology, Mesra, Ranchi 835215, Jharkhand, India;UGC Centre of Advanced Study, Department of Botany, the University of Burdwan, Golapbag, Bardhaman 713104, West Bengal, India

Received Date: 2014-07-16
Rev Recd Date: 2015-01-12

Abstract

Abstract

Fifty-seven bacteria were isolated from Southern Ocean (Indian sector) water samples which were collected from different latitude and longitude of the ocean. All the isolates were able to grow at 4℃, 20℃, 37℃ and tolerable NaCl concentration up to 13.5% (w/v). 29 out of 57 isolates were identified using 16S rDNA amplification and the sequences were submitted to National Center for Biotechnology Information (NCBI). All the isolates were classified by using Ribosomal Database Project (RDP) and found that isolates belongs to Proteobacteria and Bacteriodes. The average G+C content was 56.4%. The isolates were screened for the presence of extracellular enzymes, viz. amylase, catalase, urease, esterase, lipase and protease. The disc diffusion method is used to screen antibiotic production by the isolates against four pathogenic bacteria, viz. Salmonella typhimurium (NCIM 2501), Staphylococcus aureus (NCIM 2122), Bacillus subtilis (NCIM 2193), and Pseudomonas aeruginosa (NCIM 2036). Nine out of 29 were found to be antibiotic producer.
- Southern Ocean,
- marine bacteria,
- 16S rDNA,
- phylogenetic tree,
- antibacterial

FullText(HTML)

References(41)

References

Amann R I, Ludwig W, Schleifer K H. 1995. Phylogenetic identifica-tion and in situ detection of individual microbial cells without cultivation. Microbiology and Molecular Biology Reviews, 59(1): 143-169

Antony R, Krishnan K P, Laluraj C M, et al. 2012. Diversity and physiology of culturable bacteria associated with a coastal Ant-arctic ice core. Microbiological Research, 167(6): 372-380

Basilio A, González I, Vincente M F, et al. 2003. Patterns of antimicro-bial activities from soil actinomycetes isolated under different conditions of pH and salinity. Journal of Applied Microbiology, 93(4): 814-823

Campbell I B, Claridge G G C. 1987. Antarctica: Soils, Weathering Processes and Environment, (Developments in Soil Science 16). Amsterdam, the Netherlands: Elsevier

Chakravorty S, Helb D, Burday M, et al. 2007. A detailed analysis of 16S ribosomal RNA gene segments for the diagnosis of patho-genic bacteria. Journal of Microbiological Methods, 69(2): 330-339

Demain A L, Sanchez S. 2009. Microbial drug discovery: 80 Years of progress. The Journal of Antibiotics, 62: 5-16

Giebel H A, Brinkhoff T, Zwisler W, et al. 2009. Distribution of Roseo-bacter RCA and SAR11 lineages and distinct bacterial com-munities from the subtropics to the Southern Ocean. Environ-mental Microbiology, 11(8): 2164-2178

Gupta P, Agrawal HK, Bandopadhyay R. 2015. Distribution pattern of bacteria in the two geographic poles and Southern Ocean from the reported 16S rDNA sequences. Current Science, 108(10): 1926-1930

Gupta P, Sinha D, Bandopadhyay R. 2014. Isolation and screening of marine microalgae Chlorella sp. PR1 for anticancer activity. In-ternational Journal of Pharmacy and Pharmaceutical Sciences, 6(10): 517-519

Holding A J, Collee J G. 1971. Routine biochemical tests. Methods in Microbiology, 6A: 2-32

Jannasch H W, Jones G E. 1959. Bacterial populations in sea water as determined by different methods of enumeration. Limnology and Oceanography, 4(2): 128-139

Joseph B, Ramteke P W, Thomas G. 2008. Cold active microbial lipases: Some hot issues and recent developments. Biotechno-logy Advances, 26(5): 457-470

Kogure K. 1998. Bioenergetics of marine bacteria. Current Opinion in Biotechnology, 9(3): 278-282

Lightfield J, Fram N R, Ely B. 2011. Across bacterial phyla, distantly-related genomes with similar genomic GC content have similar patterns of amino acid usage. PLoS One, 6(3): e17677

Lordan S, Ross R P, Stanton C. 2011. Marine bioactives as functional food ingredients: potential to reduce the incidence of chronic diseases. Marine Drugs, 9(6): 1056-1100

Mearns-Spragg A, Bregu M, Boyd K G, et al. 1998. Cross-species in-duction and enhancement of antimicrobial activity produced by epibiotic bacteria from marine algae and invertebrates, after exposure to terrestrial bacteria. Letters in Applied Microbio-logy, 27(3): 142-146

Murray A E, Grzymski J J. 2007. Diversity and genomics of Antarctic marine micro-organisms. Philosophical Transactions of the Royal Society B Biological Science, 362(1488): 2259-2271

Nam E S, Ahn J K. 2011. Antarctic marine bacterium Pseudoalteromo-nas sp. KNOUC808 as a source of cold-adapted lactose hydro-lyzing enzyme. Brazilian Journal of Microbiology, 42(3): 927-936

Neufeld J D, Yu Zhongtang, Lam W, et al. 2004. Serial analysis of ri-bosomal sequence tags (SARST): a high-throughput method for profiling complex microbial communities. Environmental Mi-crobiology, 6(2): 131-144

Oh S, Kogure K, Ohwada K, et al. 1991. Correlation between posses-sion of a respiration-dependent Na+ pump and Na+ require-ment for growth of marine bacteria. Applied and Environment-al Microbiology, 57(6): 1844-1846

Orsi A H, Whitworth T III, Nowlin W D Jr. 1995. On the meridional ex-tent and fronts of the Antarctic Circumpolar Current. Deep-Sea Research Part I: Oceanographic Research Papers, 42(5): 641-673

Petti C A, Polage C R, Schreckenberger P. 2005. The role of 16S rRNA gene sequencing in identification of microorganisms misidenti-fied by conventional methods. Journal of Clinical Microbiology, 43(12): 6123-6125

Rasmussen R S, Morrissey M T. 2007. Marine biotechnology for pro-duction of food ingredients. Advances in Food and Nutrition Research, 52: 237-292

Saadoun I, Hameed K M, Moussauui A. 1999. Characterization and analysis of antibiotic activity of some aquatic actinomycetes. Microbios, 99(394): 173-179

Schmalenberger A, Schwieger F, Tebbe C C. 2001. Effect of primers hybridizing to different evolutionarily conserved regions of the small-subunit rRNA gene in PCR-based microbial community analyses and genetic profiling. Applied and Environmental Mi-crobiology, 67(8): 3557-3563

Shivaji S, Begum Z, Rao S S S N, et al. 2013. Antarctic ice core samples: culturable bacterial diversity. Research in Microbio-logy, 164(1): 70-82

Struvay C, Feller G. 2012. Optimization to low temperature activity in psychrophilic enzymes. International Journal of Molecular Sci-ence, 13(9): 11643-11665

Sokolov S, Rintoul S R. 2009. Circumpolar structure and distribution of the Antarctic Circumpolar Current fronts: 2. Variability and relationship to sea surface height. Journal of Geophysical Re-search, 114(C11): C11019

Srinivas T N R, Rao S S S N, Reddy P V V, et al. 2009. Bacterial di-versity and bioprospecting for cold-active lipases, amylases and proteases, from culturable bacteria of Kongsfjorden and Ny-Ålesund, Svalbard, Arctic. Current Microbiology, 59(5): 537-547

Steven B, Briggs G, McKay C P, et al. 2007. Characterization of the mi-crobial diversity in a permafrost sample from the Canadian high Arctic using culture-dependent and culture-independent methods. FEMS Microbiology Ecology, 59(2): 513-523

Tamura K, Peterson D, Peterson N, et al. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evol-utionary distance, and maximum parsimony method. Molecu-lar Biology and Evolution, 28(10): 2731-2739

Teixeira L C R S, Peixoto R S, Cury J C, et al. 2010. Bacterial diversity in rhizosphere soil from Antarctic vascular plants of Admiralty Bay, maritime Antarctica. The ISME Journal, 4: 989-1001

Vijayakumar A, Duraipandiyan V, Jeyaraj B, et al. 2012. Phytochemic-al analysis and in vitro antimicrobial activity of Illicium griffith-ii Hook. f. && Thoms extracts. Asian Pacific Journal of Tropical Disease, 2(3): 190-199

Wang Qiong, Garrity G M, Tiedje J M, et al. 2007. Na.ve bayesian clas-sifier for rapid assignment of rRNA sequences into the new bac-terial taxonomy. Applied and Environmental Microbiology, 73(16): 5261-5267

Ward P, Whitehouse M, Brandon M, et al. 2003. Mesozooplankton community structure across the Antarctic Circumpolar Cur-rent to the north of South Georgia: Southern Ocean. Marine Biology, 143(1): 121-130

Weber T S, Deutsch C. 2010. Ocean nutrient ratios governed by plankton biogeography. Nature, 467: 550-554

Whitworth T III. 1980. Zonation and geostrophic flow of the Antarctic circumpolar current at Drake Passage. Deep-Sea Research Part ture in the Sulu Sea and adjacent areas. Deep-Sea Research II:

A. Oceanographic Research Papers, 27(7): 497-507 Topical Studies in Oceanography, 54(1-2): 103-113

Wilkins D, Lauro F M, Williams T J, et al. 2013. Biogeographic parti-Zhang Chen, Kim S K. 2010. Research and application of marine mi-tioning of Southern Ocean microorganisms revealed by meta-crobial enzymes: status and prospects. Marine Drugs, 8(6): genomics. Environmental Microbiology, 15(5): 1318-1333 1920-1934

Wong C M V L, Tam H K, Alias S A, et al. 2011. Pseudomonas and Pe-Zhang Laitao, Zhang Wei, Jin Yan, et al. 2008. A comparative study on dobacter isolates from King George Island inhibited the growth the phylogenetic diversity of culturable actinobacteria isolated of foodborne pathogens. Polish Polar Research, 32(1): 3-14 from five marine sponge species. Antonie van Leeuwenhoek,

Yoshida A, Nishimura M, Kogure K. 2007. Bacterial community struc-93(3): 241-248

Relative Articles

Supplements(0)

Cited By

Proportional views