Microbial community structure and nitrogenase gene diversity of sediment from a deep-sea hydrothermal vent field on the Southwest Indian Ridge

Wu Yuehong; Cao Yi; Wang Chunsheng; Wu Min; Aharon Oren; Xu Xuewei

doi:10.1007/s13131-014-0544-0

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Article Navigation > Acta Oceanologica Sinica > 2014 > 33(10): 94-104

Wu Yuehong, Cao Yi, Wang Chunsheng, Wu Min, Aharon Oren, Xu Xuewei. Microbial community structure and nitrogenase gene diversity of sediment from a deep-sea hydrothermal vent field on the Southwest Indian Ridge[J]. Acta Oceanologica Sinica, 2014, 33(10): 94-104. doi: 10.1007/s13131-014-0544-0

Citation:

Wu Yuehong, Cao Yi, Wang Chunsheng, Wu Min, Aharon Oren, Xu Xuewei. Microbial community structure and nitrogenase gene diversity of sediment from a deep-sea hydrothermal vent field on the Southwest Indian Ridge[J]. Acta Oceanologica Sinica, 2014, 33(10): 94-104. doi: 10.1007/s13131-014-0544-0

Citation:

Wu Yuehong, Cao Yi, Wang Chunsheng, Wu Min, Aharon Oren, Xu Xuewei. Microbial community structure and nitrogenase gene diversity of sediment from a deep-sea hydrothermal vent field on the Southwest Indian Ridge[J]. Acta Oceanologica Sinica, 2014, 33(10): 94-104. doi: 10.1007/s13131-014-0544-0

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Microbial community structure and nitrogenase gene diversity of sediment from a deep-sea hydrothermal vent field on the Southwest Indian Ridge

doi: 10.1007/s13131-014-0544-0

1.
Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, China
2.
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
3.
College of Life Sciences, Zhejiang University, Hangzhou 310058, China
4.
Department of Plant and Environmental Sciences, the Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel;The Hebrew University of Jerusalem, The Edmond J. Safra Campus, Givat Ram, Jerusalem 91904, Israel

Received Date: 2013-03-25
Rev Recd Date: 2013-11-02

Abstract

Abstract

A sediment sample was collected from a deep-sea hydrothermal vent field located at a depth of 2 951 m on the Southwest Indian Ridge. Phylogenetic analyses were performed on the prokaryotic community using polymerase chain reaction (PCR) amplification of the 16S rRNA and nifH genes. Within the Archaea, the dominant clones were from marine benthic group E (MBGE) and marine group I (MGI) belonging to the phyla Euryarchaeota and Thaumarchaeota, respectively. More than half of the bacterial clones belonged to the Proteobacteria, and most fell within the Gammaproteobacteria. No epsilon Proteobacterial sequence was observed. Additional phyla were detected including the Actinobacteria, Bacteroidetes, Planctomycetes, Acidobacteria, Nitrospirae, Chloroflexi, Chlorobi, Chlamydiae, Verrucomicrobia, and candidate divisions OD1, OP11, WS3 and TM6, confirming their existence in hydrothermal vent environments. The detection of nifH gene suggests that biological nitrogen fixation may occur in the hydrothermal vent field of the Southwest Indian Ridge. Phylogenetic analysis indicated that only Clusters I and III nifH were present. This is consistent with the phylogenetic analysis of the microbial 16S rRNA genes, indicating that Bacteria play the main role in nitrogen fixation in this hydrothermal vent environment.
- deep-sea,
- hydrothermal vent,
- microbial diversity,
- 16S rRNA gene,
- nifH gene

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References(68)

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