Morphological characteristics and DNA barcoding of Pampus echinogaster (Basilewsky, 1855)

LI Yuan; ZHANG Yan; GAO Tianxiang; HAN Zhiqiang; LIN Longshan; ZHANG Xiumei

doi:10.1007/s13131-017-1124-x

镰鲳（Basilewsky，1855）形态特征和DNA条形码研究

doi: 10.1007/s13131-017-1124-x

1.
国家海洋局第三海洋研究所, 厦门 361005, 中国
2.
中国水产科学研究院黄海水产研究所, 青岛 266071, 中国
3.
浙江海洋大学国家海洋设施养殖工程技术研究中心, 舟山 316004, 中国

基金项目: The National Natural Science Foundation of China under contract No. 41776171; the National Programme on Global Change and Air-Sea Interaction under contract Nos GASI-02-SCS-YSWspr/aut and GASI-02-PAC-YDsum/aut; the Scientific Research Foundation of TIO, SOA under contract No. 2016010; the Bilateral Cooperation of Maritime Affairs under contract No. 2200207.

计量
- 文章访问数: 1247
- HTML全文浏览量: 110
- PDF下载量: 1109
- 被引次数: 0
出版历程
- 收稿日期: 2017-03-06

Morphological characteristics and DNA barcoding of Pampus echinogaster (Basilewsky, 1855)

1.
Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China
2.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
3.
National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan 316004, China

摘要

摘要: 鲳属鱼类相似的外部形态特征导致其种类鉴定非常混乱，其中镰鲳模式标本的准确信息和DNA条形码更是无法获得。2010年6月到2013年4月期间，我们在丹东、东营、青岛、南通、舟山、温州、长乐、台湾及和歌山县（日本）收集了207尾镰鲳样品。基于上述样品归纳了镰鲳的主要形态鉴别特征为：背鳍Ⅷ-Ⅺ-43~51；臀鳍Ⅴ-Ⅷ-43~49；胸鳍22~27；尾鳍19~22，腹鳍缺失。第一鳃耙细弱，稀疏，3~4+12~16=15-20。脊椎骨数为39~41。头部后上方侧线管的横枕管丛和背分支丛后缘呈弧形，略短，背部横枕管丛末端未达胸鳍上部基点。腹部横枕管丛稀少，明显短于背部横枕管丛。通过比较NCBI上下载的COI同源序列，两个显著的组群被检测到，研究结果显示在氨基酸序列和组群间遗传距离的基础上，该两大组群实为两个有效种，其中组群1的序列可以作为镰鲳的DNA条形码。本论文提出镰鲳正确的外部形态特征和DNA条形码，可为后续鲳属鱼类的研究提供高效、准确的保障，也为其分类提供理论基础，并能鲳属鱼类的渔业管理和保护策略提供合理的参考。
- 形态特征 /
- DNA条形码 /
- 镰鲳 /
- 遗传分化
Abstract: The morphological similarities of Pampus fishes have led to considerable confusion in species-level identification, and no accurate information on neotype or DNA barcoding of Pampus echinogaster is available. Two hundred and seven specimens of P. echinogaster were collected from the coastal waters of Dandong, Dongying, Qingdao, Nantong, Zhoushan, Wenzhou, Changle, Taiwan, and Wakayama (Japan), from June 2010 to April 2013. The diagnostic characteristics of P. echinogaster are as follows: dorsal fin VIII-XI-43-51, anal fin V-VIII-43-49, pectoral fin 22-27, caudal fin 19-22, pelvic fin absent; first gill rakers sparse, slender (pointed), 3-4+12-16=15-20; vertebrae 39-41; transverse occipital canal on top of head moderately small, wavy ridges not reaching upper origin of pectoral fin; ventral branch of lateral line canal spare, shorter than dorsal branch of lateral line canal. By combining congener sequences of the cytochrome oxidase I (COI) gene from GenBank, two absolute groups were detected among all specimens, which further indicated that two valid species were present based on genetic differences in amino acid sequences and the distance between the groups. The sequences of Group 1 can be regarded as DNA barcoding of P. echinogaster. The correct morphological redescription and DNA barcoding of P. echinogaster are presented here to provide a guarantee for efficient and accurate studies, a theoretical basis for classification, and enable appropriate fishery management and conservation strategies for the genus Pampus in the future.
- morphological characteristics /
- DNA barcoding /
- Pampus echinogaster /
- genetic differentiation

HTML全文

参考文献(35)

Abe T, Kosakai T. 1964. Notes on an economically important but scientifically little-known silver pomfret, Pampus echinogaster (Pampidae, Teleostei). Japanese Journal of Ichthyology, 12(1-2): 29-31

Cheng Qingtai. 1962. Fishes of South China Sea (in Chinese). Beijing: China Ocean Press, 759-766

Cui Zhaoxia, Liu Yuan, Liu Jing, et al. 2010. Molecular identification of Pampus fishes (Perciformes, Stromateidae). Ichthyological Research, 57(1): 32-39

Dolganov V N, Kharin V E, Zemnukhov V V. 2007. Species composition and distribution of butterfishes (Stromateidae) in waters of Russia. Journal of Ichthyology, 47(8): 579-584

Domingues R R, De Amorim A F, Hilsdorf A W S. 2013. Genetic identification of Carcharhinus sharks from the Southwest Atlantic Ocean (Chondrichthyes: Carcharhiniformes). Journal of Applied Ichthyology, 29(4): 738-742

Elliott N G, Haskard K, Koslow J A. 1995. Morphometric analysis of orange roughy (Hoplostethus atlanticus) off the continental slope of southern Australia. Journal of Fish Biology, 46(2): 202-220

Guo Enmian, Liu Yuan, Liu Jing, et al. 2010. DNA barcoding discriminates Pampus minor (Liu et al., 1998) from Pampus species. Chinese Journal of Oceanology and Limnology, 28(6): 1266-1274

Hajibabaei M, Singer G A C, Hebert P D N, et al. 2007. DNA barcoding: how it complements taxonomy, molecular phylogenetics and population genetics. Trends in Genetics, 23(4): 167-172

Hebert P D N, Cywinska A, Ball S L, et al. 2003a. Biological identifications through DNA barcodes. Proceedings of the Royal Society B: Biological Sciences, 270(1512): 313-321

Hebert P D N, Ratnasingham S, De Waard J R. 2003b. Barcoding animal life: cytochrome c oxidase subunit I divergences among closely related species. Proceedings of the Royal Society B: Biological Sciences, 270(S1): 96-99

Hubert N, Espiau B, Meyer C, et al. 2015. Identifying the ichthyoplankton of a coral reef using DNA barcodes. Molecular Ecology Resources, 15(1): 57-67

Ko H L, Wang Y T, Chiu T S, et al. 2013. Evaluating the accuracy of morphological identification of larval fishes by applying DNA barcoding. PLoS One, 8(1): e53451, doi: 10.1371/journal.pone.0053451

Li Mingde. 1998. Fish Taxonomy (in Chinese). Beijing: China Ocean Press, 230-231

Li Yuan, Song Na, Khan F S, et al. 2013. New evidence of morphological characters and DNA barcoding of Pampus argenteus (Euphrasen, 1788). Journal of Fisheries of China (in Chinese), 37(11): 1601-1608

Liu Jing, Li Chunsheng. 1998. Redescription of a stromateoid fish Pampus punctatissimus and comparison with Pampus argenteus from Chinese coastal waters. Chinese Journal of Oceanology and Limnology, 16(2): 161-166

Liu Jing, Li Chunsheng, Ning Ping. 2013. Identity of silver pomfret Pampus argenteus (Euphrasen, 1788) based on specimens from its type locality, with a neotype designation (Teleostei, Stromateidae). Acta Zootaxonomica Sinica, 38(1): 171-177

Liu Jing, Li Chunsheng. 2013. A new species of the genus Pampus (Perciformes, Stromateidae) from China. Acta Zootaxonomica Sinica, 38(4): 885-890

Meyer A. 1993. Evolution of mitochondrial DNA in fishes. In: Mochachka P K, Mommsen T P, eds. Biochemistry and Molecular Biology of Fishes. Amsterdam: Elsevier Press, 1-36

Nakabo T. 2002. Fishes of Japan with Pictorial Keys to the Species, Second Edition. Kanagawa: Tokai University Press, 961-962

Oh C W, Na J H, Kim J K. 2009. Population biology of Korean pomfret Pampus echinogaster (Basilewsky, 1855) (Perciformes: Stromateidae) on the western coast of Korea, Yellow Sea. Animal Cells and Systems, 13(1): 83-89

Peng Shiming, Shi Zhaohong, Hou Junli, et al. 2009. Genetic diversity of silver pomfret (Pampus argenteus) populations from the China Sea based on mitochondrial DNA control region sequences. Biochemical Systematics and Ecology, 37(5): 626-632

Puckridge M, Andreakis N, Appleyard S A, et al. 2013. Cryptic diversity in flathead fishes (Scorpaeniformes: Platycephalidae) across the Indo-West Pacific uncovered by DNA barcoding. Molecular Ecology Resources, 13(1): 32-42

Qin Yan, Song Na, Zou Jianwei, et al. 2013. A new record of a flathead fish (Teleostei: Platycephalidae) from China based on morphological characters and DNA barcoding. Chinese Journal of Oceanology and Limnology, 31(3): 617-624

Sun Peng, Yin Fei, Shi Zhaohong, et al. 2013. Genetic structure of silver pomfret (Pampus argenteus (Euphrasen, 1788)) in the Arabian Sea, Bay of Bengal, and South China Sea as indicated by mitochondrial COI gene sequences. Journal of Applied Ichthyology, 29(4): 733-737

Tamura K, Peterson D, Peterson N, et al. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28(10): 2731-2739

Ward R D, Zemlak T S, Innes B H, et al. 2005. DNA barcoding Australia's fish species. Philosophical Transactions of the Royal Society B: Biological Sciences, 360(1462): 1847-1857

Xiao Jiaguang, Song Na, Han Zhiqiang, et al. 2016. Description and DNA barcoding of a new Sillago species, Sillago shaoi (Perciformes: Sillaginidae), in the Taiwan Strait. Zoological Studies, 55(47), doi: 10.6620/ZS.2016.55-47

Yamada U, Tagawa M, Kishida S, et al. 1986. Fishes of the East China Sea and the Yellow Sea. Nagasaki: Seikai Regional Fisheries Research Laboratory, 280-283

Yamada U, Tokimura M, Hoshino K, et al. 2009. Names and Illustrations of Fishes from the East China Sea and the Yellow Sea—Japanese/Chinese/Korean. Tokyo, Japan: Overseas Fishery Cooperation Foundation of Japan, 435

Yang Wenhua, Cheng Qingtai. 1987. Retrieval China Fish System (in Chinese). Beijing: China Ocean Press, 425

Zemlak T S, Ward R D, Connell A D, et al. 2009. DNA barcoding reveals overlooked marine fishes. Molecular Ecology Resources, 9(S1): 237-242

Zhang Chunlin, Cheng Qingtai, Zheng Baoshan, et al. 1994. Fishes of Yellow Sea and Bohai Sea (in Chinese). Keelung: Aquatic Press, 195-196

Zhang Junbin, Hanner R. 2012. Molecular approach to the identification of fish in the South China sea. PLoS One, 7(2): e30621, doi: 10.1371/journal.pone.0030621

Zhao Feng, Dong Yinghui, Zhuang Ping, et al. 2011. Genetic diversity of silver pomfret (Pampus argenteus) in the Southern Yellow and East China Seas. Biochemical Systematics and Ecology, 39(2): 145-150

Zhu Yuanding, Zhang Chunlin, Cheng Qingtai. 1963. Fishes of East China Sea (in Chinese). Beijing: China Ocean Press, 407-410

施引文献

资源附件(0)

访问统计

点击查看大图

计量

文章访问数: 1247
HTML全文浏览量: 110
PDF下载量: 1109
被引次数: 0

镰鲳（Basilewsky，1855）形态特征和DNA条形码研究

doi: 10.1007/s13131-017-1124-x

计量

出版历程

Morphological characteristics and DNA barcoding of Pampus echinogaster (Basilewsky, 1855)

计量

出版历程

目录