Response of microbial biomass and bacterial community composition to fertilization in a salt marsh in China

MA Yuexin; TAO Wei; LIU Changfa; LIU Jiao; YANG Zhiping; LI Jin; LIU Jichen

doi:10.1007/s13131-017-1048-5

施肥对盐沼沉积物微生物生物量和细菌群落组成的影响

doi: 10.1007/s13131-017-1048-5

1.
农业部海洋水产增养殖学重点开放实验室, 大连 116023
2.
辽宁省高校近岸海洋环境科学与技术重点实验室, 大连 116023
3.
大连汇新钛设备开发有限公司, 大连 116039
4.
盘锦海洋与水产研究所, 盘锦 124010

基金项目: The National Natural Science Foundation of China under contract No. 41171389; the Public Science and Technology Research Funds Projects of Ocean under contract No. 201305043; Program for Liaoning Excellent Talents in University under contract No. LR2013035.

计量
- 文章访问数: 649
- HTML全文浏览量: 46
- PDF下载量: 309
- 被引次数: 0
出版历程
- 收稿日期: 2016-01-16
- 修回日期: 2016-08-02

Response of microbial biomass and bacterial community composition to fertilization in a salt marsh in China

1.
Key Laboratory of Mariculture and Stock Enhancement in North China's Sea of Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China
2.
Key Laboratory of Marine Environmental Research of Liaoning Higher Education, Dalian 116023, China
3.
Dalian Huixin Titanium Equipment Development Company Limited, Dalian 116039, China
4.
Institute of Ocean and Fisheries of Panjin, Panjin 124001, China

摘要

摘要: 通过氯仿熏蒸提取法、实时荧光定量PCR和变性梯度凝胶电泳（DGGE）研究了施氮（N）对双台河口翅碱蓬生长区盐沼沉积物微生物生物量、细菌丰度和群落组成的影响。于2013年7月、8月、9月和11月植物生长季节采集和分析经不同N量（尿素：0.1、0.2、0.4和0.8 g/kg（以干沉积物中N计））和不同N形式（尿素、（NH₄）₂SO₄和NH₄NO₃各0.2 g/kg）1、2、3和4次处理后的沉积物及未经处理的对照样品。结果表明，N量和N形式对微生物生物量碳、微生物生物量氮和细菌丰度产生正影响。DGGE图谱显示，N量和N形式也影响细菌群落组成。研究表明，短期施氮能增加微生物生物量和细菌丰度，并且改变细菌群落结构。
- 施肥 /
- 微生物生物量 /
- 16SrRNA基因丰度 /
- 细菌群落 /
- 盐沼
Abstract: The effects of nitrogen (N) addition on microbial biomass, bacterial abundance, and community composition in sediment colonized by Suaeda heteroptera were examined by chloroform fumigation extraction method, real-time quantitative polymerase chain reaction, and denaturing gradient gel electrophoresis (DGGE) in a salt marsh located in Shuangtai Estuary, China. The sediment samples were collected from plots treated with different amounts of a single N fertilizer (urea supplied at 0.1, 0.2, 0.4 and 0.8 g/kg (nitrogen content in sediment) and different forms of N fertilizers (urea, (NH₄)₂SO₄, and NH₄NO₃, each supplied at 0.2 g/kg (calculated by nitrogen). The fertilizers were applied 1-4 times during the plant-growing season in May, July, August, and September of 2013. Untreated plots were included as a control. The results showed that both the amount and form of N positively influenced microbial biomass carbon, microbial biomass nitrogen, and bacterial abundance. The DGGE profiles revealed that the bacterial community composition was also affected by the amount and form of N. Thus, our findings indicate that short-term N amendment increases microbial biomass and bacterial abundance, and alters the structure of bacterial community.
- fertilization /
- microbial biomass /
- 16S rRNA gene abundance /
- bacterial community /
- salt marsh

HTML全文

参考文献(39)

Alon A, Steinberger Y. 1999. Effect of nitrogen amendments on microbial biomass, above-ground biomass and nematode population in the Negev Desert soil. J Arid Environ, 41(4):429-441

Barbier E B, Hacker S D, Kennedy C, et al. 2011. The value of estuarine and coastal ecosystem services. Ecol Monogr, 81(2):169-193

Bowen J L, Byrnes J E K, Weisman D, et al. 2013. Functional gene pyrosequencing and network analysis:an approach to examine the response of denitrifying bacteria to increased nitrogen supply in salt marsh sediments. Front Microbiol, 4:342

Bowen J L, Crump B C, Deegan L A, et al. 2009. Salt marsh sediment bacteria:their distribution and response to external nutrient inputs. ISME J, 3(8):924-934

Bowen J L, Valiela I. 2004. Nitrogen loads to estuaries:using loading models to assess the effectiveness of management options to restore estuarine water quality. Estuaries, 27(3):482-500

Bowen J L, Ward B B, Morrison H G, et al. 2011. Microbial community composition in sediments resists perturbation by nutrient enrichment. ISME J, 5(9):1540-1548

Chinnadurai C, Gopalaswamy G, Balachandar D. 2014. Long term effects of nutrient management regimes on abundance of bacterial genes and soil biochemical processes for fertility sustainability in a semi-arid tropical Alfisol. Geoderma, 232-234:563-572

Cole M L, Kroeger K D, McClelland J W, et al. 2006. Effects of watershed land use on nitrogen concentrations and δ¹⁵ nitrogen in groundwater. Biogeochemistry, 77(2):199-215

Compton J E, Watrud L S, Porteous L A, et al. 2004. Response of soil microbial biomass and community composition to chronic nitrogen additions at Harvard forest. Forest Ecol Manag, 196(1):143-158

Cusack D F, Silver W L, Torn M S, et al. 2011. Changes in microbial community characteristics and soil organic matter with nitrogen additions in two tropical forests. Ecology, 92(3):621-632

Dang Hongyue, Li Jing, Chen Ruipeng, et al. 2010. Diversity, abundance, and spatial distribution of sediment ammonia-oxidizing betaproteobacteria in response to environmental gradients and coastal eutrophication in Jiaozhou Bay, China. Appl Environ Microbiol, 76(14):4691-4702

Deegan L A, Johnson D S, Warren R S, et al. 2012. Coastal eutrophication as a driver of salt marsh loss. Nature, 490(7420):388-392

Fitch R, Theodose T, Dionne M. 2009. Relationships among upland development, nitrogen, and plant community composition in a Maine salt marsh. Wetlands, 29(4):1179-1188

Gedan K B, Silliman B R, Bertness M D. 2009. Centuries of human-driven change in salt marsh ecosystems. Ann Rev Mar Sci, 1(1):117-141

Howarth R W, Sharpley A, Walker D. 2002. Sources of nutrient pollution to coastal waters in the United States:implications for achieving coastal water quality goals. Estuaries, 25(4b):656-676

Irvine I C, Vivanco L, Bentley P N, et al. 2012. The effect of nitrogen enrichment on C₁-cycling microorganisms and methane flux in salt marsh sediments. Front Microbiol, 3:90

Lage M D, Reed H E, Weihe C, et al. 2010. Nitrogen and phosphorus enrichment alter the composition of ammonia-oxidizing bacteria in salt marsh sediments. ISME J, 4(7):933-944

Li Lujun, Zeng Dehui, Yu Zhanyuan, et al. 2010. Soil microbial properties under N and P additions in a semi-arid, sandy grassland. Biol Fertil Soils, 46(6):653-658

Liu Weixing, Xu Wenhua, Han Yi, et al. 2007. Responses of microbial biomass and respiration of soil to topography, burning, and nitrogen fertilization in a temperate steppe. Biol Fertil Soils, 44(2):259-268

Luo Zhuanxi, Qiu Zhaozheng, Wei Qunshan, et al. 2014. Dynamics of ammonia-oxidizing archaea and bacteria in relation to nitrification along simulated dissolved oxygen gradient in sediment-water interface of the Jiulong river estuarine wetland, China. Environ Earth Sci, 72(7):2225-2237

Muyzer G, de waal E C, Uitterlinden A G. 1993. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol, 59(3):695-700

Nicol G W, Webster G, Glover L A, et al. 2004. Differential response of archaeal and bacterial communities to nitrogen inputs and pH changes in upland pasture rhizosphere soil. Environ Microbiol, 6(8):861-867

Peng Xuefeng, Yando E, Hildebrand E, et al. 2012. Differential responses of ammonia-oxidizing archaea and bacteria to long-term fertilization in a New England salt marsh. Front Microbiol, 3:445

Ramirez K S, Craine J M, Fierer N. 2012. Consistent effects of nitrogen amendments on soil microbial communities and processes across biomes. Global Change Biol, 18(6):1918-1927

Ramirez K S, Lauber C L, Knight R, et al. 2010. Consistent effects of nitrogen fertilization on soil bacterial communities in contrasting systems. Ecology, 91(12):3463-3470

Shen Jupei, Zhang Limei, Guo Junfu, et al. 2010. Impact of long-term fertilization practices on the abundance and composition of soil bacterial communities in Northeast China. Appl Soil Ecol, 46(1):119-124

Tamura K, Dudley J, Nei M, et al. 2007. MEGA4:Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol, 24(8):1596-1599

Treseder K K. 2008. Nitrogen additions and microbial biomass:a meta-analysis of ecosystem studies. Ecol Lett, 11(10):1111-1120

Tsuboi S, Amemiya T, Seto K, et al. 2013. The ecological roles of bacterial populations in the surface sediments of coastal lagoon environments in Japan as revealed by quantification and qualification of 16S rDNA. World J Microbiol Biotechnol, 29(5):759-774

Valiela I, Foreman K, LaMontagne M, et al. 1992. Couplings of watersheds and coastal waters:sources and consequences of nutrient enrichment in Waquoit Bay, Massachusetts. Estuaries, 15(4):443-457

Valiela I, Teal J M, Allen S D, et al. 1985. Decomposition in salt marsh ecosystems:the phases and major factors affecting disappearance of above-ground organic matter. J Exp Mar Biol Ecol, 89(1):29-54

Wallenstein M D, McNulty S, Fernandez I J, et al. 2006. Nitrogen fertilization decreases forest soil fungal and bacterial biomass in three long-term experiments. Forest Ecol Manag, 222(1-3):459-468

Wu Jinshui, Lin Qimei, Huang Qiaoyun, et al. 2006. Method and Its Application in Determination of Soil Microbial Biomass (in Chinese). Beijing:China Meteorological Press, 54-78

Xue Jinghua, Mo Jiangming, Li Jiong, et al. 2007. The short-term response of soil microorganism number to simulated nitrogen deposition. Guihaia (in Chinese), 27(2):174-179

Zak D R, Holmes W E, Tomlinson M J, et al. 2006. Microbial cycling of C and N in northern hardwood forests receiving chronic atmospheric NO^-₃ deposition. Ecosystems, 9(2):242-253

Zhang Qiufang, Peng Jingjing, Chen Qian, et al. 2011. Impacts of Spartina alterniflora invasion on abundance and composition of ammonia oxidizers in estuarine sediment. J Soil Sediment, 11(6):1020-1031

Zhang Qishui, Zak J C. 1998. Effects of water and nitrogen amendment on soil microbial biomass and fine root production in a semi-arid environment in west Texas. Soil Eiol Biochem, 30(1):39-45

Zhou Jing, Guan Dawei, Zhou Baoku, et al. 2015. Influence of 34-years of fertilization on bacterial communities in an intensively cultivated black soil in northeast China. Soil Biol Biochem, 90:42-51

Zhou Xiaobing, Zhang Yuanming, Downing A. 2012. Non-linear response of microbial activity across a gradient of nitrogen addition to a soil from the Gurbantunggut Desert, northwestern China. Soil Biol Biochem, 47:67-77.

施引文献

资源附件(0)

访问统计

点击查看大图

计量

文章访问数: 649
HTML全文浏览量: 46
PDF下载量: 309
被引次数: 0

施肥对盐沼沉积物微生物生物量和细菌群落组成的影响

doi: 10.1007/s13131-017-1048-5

计量

出版历程

Response of microbial biomass and bacterial community composition to fertilization in a salt marsh in China

计量

出版历程

目录