Identification and molecular characterization of Cathepsin L gene and its expression analysis during early ontogenetic development of kuruma shrimp Marsupenaeus japonicus

QIAO Ying WANG Jun MAO Yong LIU Min SONG Xiaohong SU Yongquan WANG Chunzhong ZHENG Zhipeng

QIAOYing, WANGJun, MAOYong, LIUMin, SONGXiaohong, SUYongquan, WANGChunzhong, ZHENGZhipeng. 日本囊对虾Cathepsin L基因的鉴定及其在幼体发育中的表达研究[J]. 海洋学报英文版, 2017, 36(6): 52-60. doi: 10.1007/s13131-017-0983-5
引用本文: QIAOYing, WANGJun, MAOYong, LIUMin, SONGXiaohong, SUYongquan, WANGChunzhong, ZHENGZhipeng. 日本囊对虾Cathepsin L基因的鉴定及其在幼体发育中的表达研究[J]. 海洋学报英文版, 2017, 36(6): 52-60. doi: 10.1007/s13131-017-0983-5
QIAO Ying, WANG Jun, MAO Yong, LIU Min, SONG Xiaohong, SU Yongquan, WANG Chunzhong, ZHENG Zhipeng. Identification and molecular characterization of Cathepsin L gene and its expression analysis during early ontogenetic development of kuruma shrimp Marsupenaeus japonicus[J]. Acta Oceanologica Sinica, 2017, 36(6): 52-60. doi: 10.1007/s13131-017-0983-5
Citation: QIAO Ying, WANG Jun, MAO Yong, LIU Min, SONG Xiaohong, SU Yongquan, WANG Chunzhong, ZHENG Zhipeng. Identification and molecular characterization of Cathepsin L gene and its expression analysis during early ontogenetic development of kuruma shrimp Marsupenaeus japonicus[J]. Acta Oceanologica Sinica, 2017, 36(6): 52-60. doi: 10.1007/s13131-017-0983-5

日本囊对虾Cathepsin L基因的鉴定及其在幼体发育中的表达研究

doi: 10.1007/s13131-017-0983-5
基金项目: The National High-tech R&D Program of China (863 Program) under contract No. 2012AA10A409-03; the Project of China Agriculture Research System under contract No. CARS-47; the Project of Xiamen Southern Ocean Research Center under contract No. 14CZY033HJ07; China Spark Program under contract No. 2015GA720002.

Identification and molecular characterization of Cathepsin L gene and its expression analysis during early ontogenetic development of kuruma shrimp Marsupenaeus japonicus

  • 摘要: 本研究从日本囊对虾(Marsupenaeus japonica)中获得半胱氨酸蛋白酶家族中的cathepsin L基因,命名为Mj-Cathepsin L。Mj-Cathepsin L基因cDNA序列全长1191bp,其开放阅读框编码的Cathepsin L蛋白前体包含327个氨基酸,由信号肽,蛋白前体和成熟蛋白三部分组成。同源序列比对发现日本囊对虾Cathepsin L蛋白与其他已知物种的cahtepsin L蛋白具有较高的同源性。对Mj-Cathepsin L基因在不同组织以及早期幼体幼体发育阶段的研究发现,Mj-Cathepsin L主要在肝胰腺中大量表达,且在幼体发育不同阶段,其表达水平受发育阶段调控,且Mj-Cathepsin L基因显示出与其五个蜕皮时期相关的表达变化,说明Mj-Cathepsin L有可能在日本囊对虾个体发育过程中扮演不同的重要作用。并通过Western Blot方法在日本囊对虾肝胰腺,胃和肠中检测到Cathepsin L酶原和成熟Cathepsin L两种蛋白存在形式。
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出版历程
  • 收稿日期:  2016-02-16
  • 修回日期:  2016-03-29

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