Genome-wide transcriptional response of the Arctic bacterium <i>Pseudoalteromonas</i> sp. A2 to oxidative stress induced by hydrogen peroxide

LIN Xuezheng; WANG Zhen; LI Yang; LI Jiang

doi:10.1007/s13131-016-0892-z

北极细菌Pseudoalteromonas sp.A2对H₂O₂胁迫的转录组分析

doi: 10.1007/s13131-016-0892-z

国家海洋局第一海洋研究所国家海洋局海洋生物活性物质重点实验室, 青岛 266061

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- 收稿日期: 2015-09-11
- 修回日期: 2015-11-25

Genome-wide transcriptional response of the Arctic bacterium Pseudoalteromonas sp. A2 to oxidative stress induced by hydrogen peroxide

Key Laboratory of Marine Bioactive Substances, the First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China

摘要

摘要: 海水中的高溶解氧浓度、低温和UV辐射导致了氧胁迫是北极海洋细菌面临的主要胁迫因素之一。本文对北极细菌Pseudoalteromonas sp.A2对H₂O₂导致的氧胁迫的应答特征进行了转录组测序和基因差异表达的比较分析，以期发现与氧胁迫相关的关键功能基因。研究表明，与对照组相比，1 mmol/L的H₂O₂可导致菌株A2转录组的很大变化，包括109个基因的显著上调与174个基因的显著下调。COG分析表明，在功能已知的基因中，与转录后修饰、蛋白质转换和分子伴侣相关的基因大部分显著上调，而与氨基酸运输和代谢等相关基因则大部分显著下调。值得指出的是，有18个与鞭毛相关的基因和4个与热激蛋白相关的基因显著上调；同时，有9个与细胞色素和细胞色素氧化酶相关的基因和5个与TonB依赖受体相关的基因显著下调。在GO分析表明具有抗氧化活性的18个基因中，只有1个基因的表达显著上调，而有2个显著下调。简言之，RNA-Seq表明，除了传统的抗氧化基因和应激蛋白外，鞭毛相关基因和功能未知基因在菌株Pseudoalteromonas sp.A2的氧胁迫适应性中起着重要作用。该转录组分析和氧胁迫相关基因的发现有助于了解北极细菌的氧胁迫适应机制。
- 北极 /
- 假交替单胞菌 /
- 氧胁迫 /
- RNA-Seq
Abstract: Oxidative stress is one of the major challenges faced by Arctic marine bacteria due to the high oxygen concentration of seawater, low temperatures and UV radiations. Transcriptome sequencing was performed to obtain the key functional genes involved in the adaptation to oxidative stress induced by hydrogen peroxide in the Arctic bacterium Pseudoalteromonas sp. A2. Exposure to 1 mmol/L H₂O₂ resulted in large alterations of the transcriptome profile, including significant up-regulation of 109 genes and significant down-regulation of 174 genes. COG functional classification revealed that among the significantly regulated genes with known function categories, more genes belonging to posttranslational modification, protein turnover and chaperones were significantly up-regulated, and more genes affiliated with chaperones and amino acid transport and metabolism were significantly down-regulated. It was notable that the expressions of eighteen genes affiliated with flagella and four genes affiliated with heat shock proteins were significantly up-regulated. Meanwhile, the expression of nine genes belonging to cytochrome and cytochrome oxidase, and five genes belonging to TonB-dependent receptor, were significantly down-regulated. Among the eighteen genes with antioxidant activity categorized by GO analysis, the expression of one gene was significantly up-regulated; however, the expressions of two genes were significantly down-regulated. Briefly, RNA-Seq indicated that, except for the classical anti-oxidative genes and stress proteins, genes affiliated with flagella and function unknown played important roles in coping with oxidative stress in Pseudoalteromonas sp. A2. This overall survey of transcriptome and oxidative stress-relevant genes can contribute to understand the adaptive mechanism of Arctic bacteria.
- Arctic /
- Pseudoalteromonas /
- oxidative stress /
- RNA-Seq

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北极细菌Pseudoalteromonas sp.A2对H2O2胁迫的转录组分析

doi: 10.1007/s13131-016-0892-z

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Genome-wide transcriptional response of the Arctic bacterium Pseudoalteromonas sp. A2 to oxidative stress induced by hydrogen peroxide

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北极细菌Pseudoalteromonas sp.A2对H₂O₂胁迫的转录组分析