Combined effects of temperature and copper ion concentration on the superoxide dismutase activity in <i>Crassostrea ariakensis</i>

WANG Hui; YANG Hongshuai; LIU Jiahui; LI Yanhong; LIU Zhigang

doi:10.1007/s13131-016-0838-5

温度和铜离子浓度对近江牡蛎超氧化物岐化酶活力的联合效应

doi: 10.1007/s13131-016-0838-5

1.
淮阴师范学院生命科学学院, 淮安 223300, 中国
2.
广东海洋大学水产学院, 湛江 524088, 中国

基金项目: The Guangdong Province Education Department under contract No.GCZX-A0909; the Guangdong Province Ocean and Fisheries Science & Technology Extension Project under contract No.20120980; the Guangdong Province Industry-University-Science Partnership Project under contract No.20110908; the Science & Technology Project of Huaiyin Normal University under contract No.WH0031.

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- 收稿日期: 2014-11-30
- 修回日期: 2015-09-14

Combined effects of temperature and copper ion concentration on the superoxide dismutase activity in Crassostrea ariakensis

1.
School of Life Sciences, Huaiyin Normal University, Huaian 223300, China
2.
Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China

摘要

摘要: 作为包括海洋无脊椎动物体内对抗氧自由基的第一道防线,超氧化物岐化酶(SOD)是一种关键抗氧化酶。该酶在真核生物中主要有两种形式,其一是铜/锌超氧化物岐化酶(SOD1),其二是锰超氧化物同岐化酶(SOD2)。SOD的活力同时受到众多环境应激因子的影响。利用中心复合设计及响应曲面方法,本研究中考察了温度(18-34℃)和铜离子浓度(0.1-1.5 mg/L)对近江牡蛎消化腺SOD活力的联合效应。结果表明,温度的一次效应极显著(P<0.01),二次效应显著(P<0.05);铜离子浓度的一次效应不显著(P>0.05),而二次效应极显著(P<0.01);二个因子间的互作不显著(P>0.05);温度的效应大于铜离子浓度的效应;建立了消化腺SOD酶活力对两个因子的模型方程,普通决定系数和预测决定系数分别达到0.9616和0.8207,表明模型拟合度较为理想并可在本试验条件下用于预测近江牡蛎消化腺SOD活力。研究结果有助于将SOD作为潜在生物指标说明海洋动物的健康状态及所面临的环境问题。
- 近江牡蛎 /
- 超氧化物岐化酶 /
- 温度 /
- 铜离子浓度 /
- 联合效应
Abstract: Superoxide dismutase(SOD) is a crucial antioxidant enzyme playing the first defense line in antioxidant pathways against reactive oxygen species in various organisms including marine invertebrates. There exist mainly two specific forms, Cu/Zn-SOD(SOD1) and Mn-SOD(SOD2), in eukaryotes. SODs are known to be concurrently modulated by a variety of environmental stressors. By using central composite experimental design and response surface method, the joint effects of water temperature(18-34℃) and copper ion concentration(0.1-1.5 mg/L) on the total SOD activity in the digestive gland of Crassostrea ariakensis were studied. The results showed that the linear effect of temperature was highly significant(P<0.01), the quadratic effect of temperature was significant(P<0.05); the linear effect of copper ion concentration was not significant(P>0.05), while the quadratic effect of copper ion concentration was highly significant(P<0.01); the interactive effect of temperature and copper ion concentration was not significant(P>0.05); the effect of temperature was greater than that of copper ion concentration. The model equation of digestive gland SOD enzyme activity towards the two factors of interest was established, with R² and predictive R² as high as 0.9616 and 0.8207, respectively, suggesting that the goodness-of-fit to experimental data be very satisfactory, and could be applied to prediction of digestive gland SOD activity in C. ariakensis under the conditions of the experiment. Our results would be conducive to addressing the health of aquatic animals and/or to detecting environmental problems by taking SOD as a potential bioindicator.
- Crassostrea ariakensis /
- superoxide dismutase /
- temperature /
- copper ion concentration /
- combined effect

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温度和铜离子浓度对近江牡蛎超氧化物岐化酶活力的联合效应

doi: 10.1007/s13131-016-0838-5

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出版历程

Combined effects of temperature and copper ion concentration on the superoxide dismutase activity in Crassostrea ariakensis

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