Patterns of genetic and morphometric diversity in the marbled crab (Pachygrapsus marmoratus, Brachyura, Grapsidae) populations across the Tunisian coast
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Abstract: The present study reports on population structure analysis of the marbled crab Pachygrapsus marmoratus (Fabricius, 1787) from the Tunisian coast, an appropriate location to study biogeographical processes because of the presence of a well-known discontinuous biogeographic area (the Siculo-Tunisian Strait). Patterns of morphological and genetic variation of this highly dispersive and continuously distributed decapod species were assessed among its geographically close populations which cover almost the entire Tunisian coastline. A total of 386 specimens from nine sites were collected and examined for morphometric variability at 14 morphometric traits. The results of multivariate analyses of linear morphometric traits showed the existence of sexual dimorphism in this species by PERMANOVA (Permutational multivariate analysis of variance). In addition, both CDA (Canonical discriminant analysis) and NPMANOVA (Non parametric MANOVA test) analyses revealed statistically significant differences among the studied locations for both sexes. Overall, the outcome of CDA analysis showed that over 87% of individuals could be assigned correctly to three regional groups in both sexes (North, Center and South). Specifically, SIMPER (Similarity Percentages) analysis showed that carapace length, carapace width and merus length were major contributors to the morphometric separation between populations. The pattern of phenotypic variation suggested by morphometric analyses was found to be highly discordant with that suggested by the analysis of a mitochondrial marker (cytochrome oxidase I, COI). Indeed, the results inferred from restriction fragment analysis of the COI in 180 crabs, suggested high genetic homogeneity. Very low levels of haplotype diversity (h) were found in almost all the studied populations, associated with non significant genetic distances for nearly all population comparisons. Explanations to these morphometric and mtDNA patterns as well as the discrepancy between them are discussed.
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Anastasiadou C, Leonardos I D. 2008. Morphological variation among populations of Atyaephyra desmarestii (Millet, 1831) (Decapoda: Caridea: Atyidae) from freshwater habitats of northwestern Greece. Journal of Crustacean Biology, 28(2):240-247 Anderson M J. 2001. A new method for non-parametric multivariate analysis of variance. Austral Ecology, 26(1): 32-46 Anderson M J. 2005. Permanova-Permutational multivariate analysis of variance [dissertation]. Auckland: Department of Statistics University of Auckland Anderson M J, Ter Braak C J F. 2003. Permutation tests for multi-factorial analysis of variance. Journal of Statistical Computa-tion and Simulation, 73(2): 85-113 Arnaud-Haond S, Migiaccio M, Diaz-Almela E, et al. 2007. Vicariance patterns in the Mediterranean Sea: east-west cleavage and low dispersal in the endemic seagrass Posidonia oceanica. Journal of Biogeography, 34 (6): 963-976 Avise J C. 2000. Phylogeography: the History and Formation of Spe-cies. Cambridge, Massachusetts: Harvard University Press, 464 Bahri-Sfar L, Lemaire C, Hassine O K B, et al. 2000. Fragmentation of sea bass populations in the western and eastern Mediter-ranean as revealed by microsatellite polymorphism. The Royal Society Publishing: Proceedings B, 267(1446): 929-935 Barber P H, Erdmann M V, Palumbi S R. 2006. Comparative phylo-geography of three codistributed stomatopods: origins and tim-ing of regional lineage diversification in the coral triangle. Evol-ution, 60(9): 1825-1839 Bell M A, Aguirre W E, Buck N J. 2004. Twelve years of contemporary armor evolution in a threespine stickleback population. Evolu-tion, 58(4): 814-824 Bentley M G, Clare A S, Scott C L, et al. 2002. A1.10-Multi-level ana-lyses of endocrine disruption in shore crabs from estuarine and coastal environments. Comparative Biochemistry and Physiology Part A, 132: S1-S12 Borsa P, Blanquer A, Berrebi P. 1997. Genetic structure of the flounders Platichthys flesus and P. stellatus at di.erent geo-graphic scales. Marine Biology, 129(2): 233-246 Brian J V. 2005. Inter-population variability in the reproductive mor-phology of the shore crab (Carcinus maenas): evidence of en-docrine disruption in a marine crustacean? Marine Pollution Bulletin, 50(4): 410-416 Brian J V, Fernandes T, Ladle R J, et al. 2006. Patterns of morphologic-al and genetic variability in UK populations of the shore crab Carcinus maenas Linnaeus, 1758 (Crustacea: Decapoda: Bra-chyura). Journal of Experimental Marine Biology and Ecology, 329(1): 47-54 Brooker A L, Benzie J A H, Blair D, et al. 2000. Population structure of the giant tiger prawn Penaeus monodon in Australian waters, determined using microsatellite markers. Marine Biology, 136(1): 149-157 Burrows M T, Harvey R, Robb L. 2008. Wave exposure indices from digital coastlines and the prediction of rocky shore community structure. Marine Ecology Progress Series, 353: 1-12 Cadrin S X. 1995. Discrimination of American lobster (Homarus americanus) stocks off southern New England on the basis of secondary sex character allometry. Canadian Journal of Fisher-ies and Aquatic Sciences, 52(12): 2712-2723 Cannicci S, Gomei M, Boddi B, et al. 2002. Feeding habits and natur-al diet of the intertidal crab Pachygrapsus marmoratus: oppor-tunistic browser or selective feeder? Estuarine, Coastal and Shelf Science, 54(6): 983-1001 Cannicci S, Gomei M, Dahdouh-Guebas F, et al. 2007. Influence of seasonal food abundance and quality on the feeding habits of an opportunistic feeder, the intertidal crab Pachygrapsus mar-moratus. Marine Biology, 151(4): 1331-1342 Cannicci S, Paula J, Vannini M. 1999. Activity pattern and spatial strategy in Pachygrapsus marmoratus (Decapoda: Grapsidae) from Mediterranean and Atlantic shores. Marine Biology, 133(3): 429-435 Cassone B J, Boulding E G. 2006. Genetic structure and phylogeo-graphy of the lined shore crab, Pachygrapsus crassipes, along the northeastern and western Pacific coasts. Marine Biology, 149(2): 213-226 Clarke K R. 1993. Non-parametric multivariate analyses of changes in community structure. Australian Journal of Ecology, 18(1): 117-143 Cuesta J A, Rodriguez A. 2000. Zoeal stages of the intertidal crab Pachygrapsus marmoratus (Fabricius, 1787) (Brachyura, Grap-sidae) reared in the laboratory. Hydrobiologia, 436(1-3): 119-130 Darling J A, Bagley M J, Roman J, et al. 2008. Genetic patterns across multiple introductions of the globally invasive crab genus (Car-cinus). Molecular Ecology, 17(23): 4992-5007 Debuse V J, Addison J T, Reynolds J D. 2001. Morphometric variabil-ity in UK populations of the European lobster. Journal of the Marine Biological Association of the United Kingdom, 81(3): 469-474 Deli T, Said K, Chatti N. 2014. Morphological differentiation among geographically close populations of the green crab Carcinus aestuarii Nardo, 1847 (Brachyura, Carcinidae) from the Tunisi-an coast. Crustaceana, 87(3): 257-283 Duarte C M, Dennison W C, Orth R J W, et al. 2008. The charisma of coastal ecosystems: addressing the imbalance. Estuaries and Coasts, 31(2): 233-238 Duffy J E. 1996. Species boundaries, specialization, and the radiation of sponge-dwelling alpheid shrimp. Biological Journal of the Linnean Society, 58(3): 307-324 Excoffier L, Laval G, Schneider S. 2005. Arlequin ver.3.0: an integ-rated software package for population genetics data analysis. Evolutionary Bioinformatics Online, 1: 47-50 Ferreira Silva A C, Braz.o S, Hawkins S J, et al. 2009. Abundance, pop-ulation structure and claw morphology of the semi-terrestrial crab Pachygrapsus marmoratus (Fabricius, 1787) on shores of differing wave exposure. Marine Biology, 156(12): 2591-2599 Flores A A V, Paula J. 2002. Population dynamics of the shore crab Pachygrapsus marmoratus (Brachyura: Grapsidae) in the cent-ral Portuguese coast. Journal of the Marine Biological Associ-ation of the United Kingdom, 82(2): 229-241 Folmer O, Black M, Hoeh W, et al. 1994. DNA primers for amplifica-tion of mitochondrial cytochrome c oxidase subunit I from di-verse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3(5): 294-299 Fratini S, Ragionieri L, Cutuli G, et al. 2013. Pattern of genetic isola-tion in the crab Pachygrapsus marmoratus within the Tuscan Archipelago (Mediterranean Sea). Marine Ecology Progress Series, 478: 173-183 Fratini S, Schubart C D, Ragionieri L. 2011. Population genetics in the rocky shore crab Pachygrapsus marmoratus from the western Mediterranean and eastern Atlantic: complementary results from mtDNA and microsatellites at different geographic scales. In: Held C, Koenemann S, Schubart C D, eds. Crustacean Is-sues 19: Phylogeography and Population Genetics in Crustacea. Boca Raton, Florida: Taylor & Francis/CRC Press, 191-213 Fratini S, Vannini M. 2002. Genetic differentiation in the mud crab Scylla serrata (Decapoda: Portunidae) within the Indian Ocean. Journal of Experimental Marine Biology and Ecology, 272(1): 103-116 Fratini S, Zane L, Ragionieri L, et al. 2008. Relationship between heavy metal accumulation and genetic variability decrease in the intertidal crab Pachygrapsus marmoratus (Decapoda; Grapsidae). Estuarine, Coastal and Shelf Science, 79(4): 679-686 Freire J, Sampedro M P, González-Gurriarán E. 1996. Influence of morphometry and biomechanics on diet selection in three por-tunid crabs. Marine Ecology Progress Series, 137: 111-121 Hammer Ø, Harper D A T, Ryan P D. 2001. PAST: Paleontological statistics software package for education and data analysis. Pa-laeontologia Electronica, 4(1): 9 Hobbs R C, Botsford L W, Thomas A. 1992. Influence of hydrographic conditions and wind forcing on the distribution and abund-ance of Dungeness crab, Cancer magister, larvae. Canadian Journal of Fisheries and Aquatic Sciences, 49(7): 1379-1388 Hopkins M J, Thurman C L. 2010. The geographic structure of mor-phological variation in eight species of fiddler crabs (Ocypodid-ae: genus Uca) from the eastern United States and Mexico. Bio-logical Journal of the Linnean Society, 100(1): 248-270 Hunt A. 1993. Effects of contrasting patterns of larval dispersal on the genetic connectedness of local populations of two intertidal starfish, Pateriella calcar and P. exigua. Marine Ecology Pro-gress Series, 92: 179-186 Ingle R W. 1980. British Crabs. British Museum (Natural History). London: Oxford University Press, 222 Johnson D F, Botsford L W, Methot R D Jr, et al. 1986. Wind stress and cycles in Dungeness crab (Cancer magister) catch off California, Oregon, and Washington. Canadian Journal of Fisheries and Aquatic Sciences, 43(4): 838-845 Johnson D R, Hester B S, McConaugha J R. 1984. Studies of a wind mechanism influencing the recruitment of blue crabs in the Middle Atlantic Bight. Continental Shelf Research, 3(4): 425-437 Jørstad K E, Prodöhl P A, Agnalt A-L, et al. 2004. Sub-arctic popula-tions of European lobster, Homarus gammarus, in northern Norway. Environmental Biology of Fishes, 69(1-4): 223-231 Kimura M, Weiss G H. 1964. The stepping stone model of population structure and the decrease of genetic correlation with distance. Genetics, 49: 561-576 Lee S Y, Seed R. 1992. Ecological implications of cheliped size in crabs: some data from Carcinus maenas and Liocarcinus holsat-us. Marine Ecology Progress Series, 84: 151-160 Lye C M, Bentley M G, Clare A S, et al. 2005. Endocrine disruption in the shore crab Carcinus maenas—a biomarker for benthic mar-ine invertebrates? Marine Ecology Progress Series, 288: 221-232 Mense D J, Posey M H, West T, et al. 1995. Settlement of brachyuran postlarvae along the North Carolina coast. Bulletin of Marine Science, 57(3): 793-806 Narum S R. 2006. Beyond Bonferroni: less conservative analyses for conservation genetics. Conservation Genetics, 7(5): 783-787 Nei M. 1987. Molecular Evolutionary Genetics. New York: Columbia University Press, 512 Nikula R, V.in.l. R. 2003. Phylogeography of Cerastoderma glaucum (Bivalvia: Cardiidae) across Europe: a major break in the East-ern Mediterranean. Marine Biology, 143(2): 339-350 Orr M R, Smith T B. 1998. Ecology and speciation. Trends Ecol Evol, 13(12): 502-506 Pfeiler E, Hurtado L A, Knowles L L, et al. 2005. Population genetics of the swimming crab Callinectes bellicosus (Brachyura: Portunid-ae) from the eastern Pacific Ocean. Marine Biology, 146(3): 559-569 Quesada H, Beynon C M, Skibinski D O F. 1995. A mitochondrial DNA discontinuity in the mussel Mytilus Galloprovincialis Lmk: pleistocene vicariance biogeography and secondary intergrada-tion. Molecular Biology and Evolution, 12(3): 521-524 Reist J D. 1985. An empirical evaluation of several univariate meth-ods that adjust for size variation in mor-phometric data. Cana-dian Journal of Zoology, 63(6): 1429-1439 Roman J, Palumbi S R. 2004. A global invader at home: population structure of the green crab, Carcinus maenas, in Europe. Mo-lecular Ecology, 13(10): 2891-2898 Schluter D. 2000. Ecological character displacement in adaptive radi-ation. Amer Nat, 156(S4): S4-S16 Schubart C D, Cuesta J A, Rodriguez A. 2001. Molecular phylogeny of the crab genus Brachynotus (Brachyura: Varunidae) based on the 16S rRNA gene. Hydrobiologia, 449(1-3): 41-46 Serena F. 2005. Field identification guide to the sharks and rays of the Mediterranean and Black Sea. FAO Species Identification Guide for Fishery Purposes. Rome: FAO, 97 Shirley S M, Shirley T C, Rice S D. 1987. Latitudinal variation in the Dungeness crab, Cancer magister: zoeal morphology explained by incubation temperature. Marine Biology, 95(3): 371-376 Silva I C, Mesquita N, Paula J. 2010. Genetic and morphological dif-ferentiation of the mangrove crab Perisesarma guttatum (Bra-chyura: Sesarmidae) along an East African latitudinal gradient. Biological Journal of the Linnean Society, 99(1): 28-46 Silva I C, Mesquita N, Schubart C D, et al. 2009. Genetic patchiness of the shore crab Pachygrapsus marmoratus along the Portuguese coast. Journal of Experimental Marine Biology and Ecology, 378(1-2): 50-57 Silva I C, Paula J. 2008. Is there a better chela to use for geometric morphometric differentiation in brachyuran crabs? A case study using Pachygrapsus marmoratus and Carcinus maenas. Journal of the Marine Biological Association of the United King-dom, 88(5): 941-953 Slatkin M, Barton N H. 1989. A comparison of three indirect methods for estimating average levels of gene flow. International Journal of Organic Evolution, 43(7): 1349-1368 Slatkin M, Maddison W P. 1990. Detecting isolation by distance using phylogenies of genes. Genetics, 126(1): 249-260 Spivak E D, Schubart C D. 2003. Species status in question: a morpho-metric and molecular comparison of Cyrtograpsus affinis and C. altimanus (Decapoda, Brachyura, Varunidae). Journal of Crustacean Biology, 23(1): 212-222 Spotte S. 1997. Sexual and regional variation in the morphology of the spotted anemone shrimp Periclimenes yucatanicus (Decapoda: Caridea: Palaemonidae). Journal of Crustacean Biology, 17(3): 389-397 Stamatis C, Triantafyllidis A, Moutou K A, et al. 2004. Mitochondrial DNA variation in Northeast Atlantic and Mediterranean popu-lations of Norway lobster Nephrops norvegicus. Molecular Eco-logy, 13(6): 1377-1390 Stamatis C, Triantafyllidis A, Moutou K A, et al. 2006. Allozymic vari-ation in Northeast Atlantic and Mediterranean populations of Norway lobster Nephrops norvegicus. ICES Journal of Marine Science, 63(5): 875-882 StatSoft, Inc. 1993. STATISTICA (data analysis software system: for the windows operating system reference for statistical proced-ures), version 4.5. www.statsoft.com Triantafyllidis A, Apostolidis A P, Katsares V, et al. 2005. Mitochondri-al DNA variation in the European lobster (Homarus gammarus) throughout the range. Marine Biology, 146(2): 223-235 Weber L I, Hartnoll R G, Thorpe J P. 2000. Genetic divergence and lar-val dispersal in two spider crabs (Crustacea: Decapoda). Hy-drobiologia, 420(1): 211-219 Weber L I, Levy J A. 2000. Genetic population structure of the swim-ming crab Callinectes danae (Crustacea: Decapoda) in south-ern Brazil. Hydrobiologia, 420(1): 203-210 Weetman D, Ruggiero A, Mariani S, et al. 2007. Hierarchical popula-tion genetic structure in the commercially exploited shrimp Crangon crangon identified by AFLP analysis. Marine Biology, 151(2): 565-575 Wright S. 1950. The genetical structure of populations. Nature, 166 (4215): 247-249 Zitari-Chatti R, Chatti N, Elouaer A, et al. 2008. Genetic variation and population structure of the caramote prawn Penaeus kerathur-us (Forskäl) from the eastern and western Mediterranean coasts in Tunisia. Aquaculture Research, 39(1): 70-76 Zitari-Chatti R, Chatti N, Fulgione D, et al. 2009. Mitochondrial DNA variation in the caramote prawn Penaeus (Melicertus) kerathur-us across a transition zone in the Mediterranean Sea. Genetica, 136(3): 439-447
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