Molecular characterization and expression analysis of a novel dual-CRD C-type lectin in kuruma shrimp (<i>Marsupenaeus japonicus</i>)

ZHANG Man; MAO Yong; WANG Jun; FENG Wenrong; SONG Xiaohong; SU Yongquan

doi:10.1007/s13131-015-0623-x

日本囊对虾一种具有双结构域的新型C-型凝集素的分子特性和表达分析

doi: 10.1007/s13131-015-0623-x

College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China

基金项目: The National High-Technology R&D Program (863 Program) of China under contract No. 2012AA10A409; China Agriculture Research System under contract No. CARS-47.

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- 收稿日期: 2013-10-05
- 修回日期: 2014-01-03

Molecular characterization and expression analysis of a novel dual-CRD C-type lectin in kuruma shrimp (Marsupenaeus japonicus)

College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China

摘要

摘要: C-型凝集素是无脊椎动物中一种重要的模式识别受体.它们是一类能够识别病原体表面特异性糖基的糖结合蛋白.本研究发现了一种新型的日本囊对虾C-型凝集素,命名为MjLectin.MjLectin cDNA 序列全长1245bp,开放阅读框长1011bp,编码336个氨基酸.不同于已报道的只含有一个糖识别结构域的日本囊对虾C-型凝集素,MjLectin具有两个串联排列的糖识别结构域.与其他含有两个结构域的对虾凝集素相比,MjLectin表现出较高的相似性.在两个结构域(CRD1和CRD2)的钙离子结合位点2中,决定糖结合特异性的三肽元件分别为突变的LPN (Leu₁₃₄-Pro₁₃₅-Asn₁₃₆) 和保守的EPN (Glu₂₉₉-Pro₃₀₀-Asn₃₀₁).MjLectin在组织和细胞水平上均具有表达特异性,主要在日本囊对虾肝胰腺的F细胞中表达.白斑综合征病毒(WSSV)攻毒后,肝胰腺中MjLectin的表达上调,并且在48小时含量极显著升高(P<0.01).以上结果表明,MjLectin可能参与了机体抵御WSSV感染的先天免疫防御.
- C-型凝集素 /
- 日本囊对虾 /
- 糖识别结构域 /
- 基因表达 /
- 白斑综合征病毒
Abstract: C-type lectins are among the most significant pattern recognition receptors (PRRs) found in invertebrate. They are a class of carbohydrate-binding proteins that can recognize specific sugar moieties on the surface of pathogens. In the present study, a novel C-type lecitn (termed MjLectin) from kuruma shrimp Marsupenaeus japonicus was identified. The full-length cDNA of MjLectin was 1 245 bp with a 1 011 bp open reading frame (ORF) that encoded a polypeptide of 336 amino acid residues. MjLectin consisted of two tandemly arrayed carbohydrate-recognition domains (CRDs), unlike other reported M. japonicus C-type lectins with only one CRD. It showed a high similarity to other shrimp dual-CRD lectins. Among the Ca²⁺-binding Site 2, the tripeptide motif dictating the carbohydrate binding specificity was exhibited as a rare mutant LPN (Leu₁₃₄-Pro₁₃₅-Asn₁₃₆) in CRD1 and a traditional EPN (Glu₂₉₉-Pro₃₀₀-Asn₃₀₁) in CRD2, respectively. MjLectin showed a specific expression pattern in both tissue and cellular levels, for its mRNA transcript was mainly expressed in the F-cells of the hepatopancreas. After white spot syndrome virus (WSSV) challenge (3.6×10⁸ virions/μL), the expression of MjLectin in the hepatopancreas was up-regulated significantly at 48 h (P<0.01) compared with the control group. These results suggested that MjLectin might be involved in the innate immune defense against WSSV infection.
- C-type lectin /
- Marsupenaeus japonicus /
- carbohydrate-recognition domain (CRD) /
- gene expression /
- white spot syndrome virus (WSSV)

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参考文献(39)

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日本囊对虾一种具有双结构域的新型C-型凝集素的分子特性和表达分析

doi: 10.1007/s13131-015-0623-x

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出版历程

Molecular characterization and expression analysis of a novel dual-CRD C-type lectin in kuruma shrimp (Marsupenaeus japonicus)

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出版历程

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