Influences of external nutrient conditions on the transcript levels of a nitrate transporter gene in <i>Skeletonema costatum</i>

LIU Yun; SONG Xiuxian; HAN Xiaofian; YU Zhiming

doi:10.1007/s13131-013-0324-2

Influences of external nutrient conditions on the transcript levels of a nitrate transporter gene in Skeletonema costatum

doi: 10.1007/s13131-013-0324-2

1.
Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Graduate School of the Chinese Academy of Sciences, Beijing 100049, China
2.
Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China

基金项目: The National Basic Research Program of China (973 Program) under contract No.2010CB428706; the National Natural Science Foundation of China for Creative Research Groups under contract No.41121064; the National High Technology Research and Development Program of China under contract No.2008AA09Z107.

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出版历程
- 收稿日期: 2012-03-23
- 修回日期: 2012-09-12

Influences of external nutrient conditions on the transcript levels of a nitrate transporter gene in Skeletonema costatum

1.
Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Graduate School of the Chinese Academy of Sciences, Beijing 100049, China
2.
Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China

摘要

摘要: To verify the feasibility of high-affinity nitrate transporter gene (Nrt2) as an indicator of nitrogen status, changes in the transcript levels of transcripts associated with phosphate starvation and different nitrate concentrations were studied using real-time quantitative reverse-transcription PCR (QRT-PCR) technology in batch cultures of Skeletonema costatum. The results show that compared with P-replete condition, P starvation could reduce the Nrt2 transcript levels apparently. Nrt2 transcript levels had a significant negative linear correlation with nitrate concentrations below 40 μmol/L. The results of 48 h short-term incubation experiment under different nitrate concentrations confirmed this correlation, and the following regression equation is built:y=-3.305x+98.95, R²=0.988, where x represents nitrate concentrations (<40 μmol/L) and y represents the Nrt2 transcript levels.
- high-affinitynitratetransportergene /
- phosphatestarvation /
- nitrateconcentrations /
- real-timePCR /
- Skeletonemacostatum
Abstract: To verify the feasibility of high-affinity nitrate transporter gene (Nrt2) as an indicator of nitrogen status, changes in the transcript levels of transcripts associated with phosphate starvation and different nitrate concentrations were studied using real-time quantitative reverse-transcription PCR (QRT-PCR) technology in batch cultures of Skeletonema costatum. The results show that compared with P-replete condition, P starvation could reduce the Nrt2 transcript levels apparently. Nrt2 transcript levels had a significant negative linear correlation with nitrate concentrations below 40 μmol/L. The results of 48 h short-term incubation experiment under different nitrate concentrations confirmed this correlation, and the following regression equation is built:y=-3.305x+98.95, R²=0.988, where x represents nitrate concentrations (<40 μmol/L) and y represents the Nrt2 transcript levels.
- high-affinity nitrate transporter gene /
- phosphate starvation /
- nitrate concentrations /
- real-time PCR /
- Skeletonema costatum

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文章访问数: 368
HTML全文浏览量: 57
PDF下载量: 96
被引次数: 0

Influences of external nutrient conditions on the transcript levels of a nitrate transporter gene in Skeletonema costatum

doi: 10.1007/s13131-013-0324-2

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出版历程

Influences of external nutrient conditions on the transcript levels of a nitrate transporter gene in Skeletonema costatum

计量

出版历程

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