Development of EST-SSR primers and their practicability test for <i>Laminaria</i>

WANG Guoliang; TAN Xiaolong; SHEN Junling; LI Jian; ZHANG Lei; SUN Jianwei; WANG Bin; WENG Manli; LIU Tao

doi:10.1007/s13131-011-0125-4

Development of EST-SSR primers and their practicability test for Laminaria

doi: 10.1007/s13131-011-0125-4

1.
College of Marine Life Science, Ocean University of China, Qingdao 266003, China;Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
2.
College of Marine Life Science, Ocean University of China, Qingdao 266003, China
3.
College of Horticulture, Qingdao Agricultural University, Qingdao 266109, China
4.
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

基金项目: The National Natural Science Foundation of China under contract No. 40776072 and 40606035; the National High Technology Research and Development Program (863 Program) of China under contract Nos 2006AA10A412 and 2010AA10A401; the Key Projects in the National Science & Technology Pillar Program under contract No. 2006BAD01A13.

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出版历程
- 收稿日期: 2010-05-10
- 修回日期: 2010-11-19

Development of EST-SSR primers and their practicability test for Laminaria

1.
College of Marine Life Science, Ocean University of China, Qingdao 266003, China;Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
2.
College of Marine Life Science, Ocean University of China, Qingdao 266003, China
3.
College of Horticulture, Qingdao Agricultural University, Qingdao 266109, China
4.
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

摘要

摘要: Laminaria (Saccharina) is one of the economic important seaweeds. Through sequence analysis of 4 099 ESTs from Laminaria (971 were generated from our own cDNA laboratories and 3 128 were downloaded from updated EST databases) with SSRIT software, two hundred and fifty-four SSRs in 206 ESTs were found. From the 254 SSRs, sixty-three SSR primer-pairs were designed. In order to test their practicability, the 63 primer-pairs were tested in commonly used SSR reaction conditions using 12 Laminara DNA samples as templates. The results show that 23 SSR primer-pairs gave good amplification patterns on most (more than 80%) of the 12 Laminara DNA templates. Genetic diversity study of 12 Laminaria lines, which were widely used in breeding and economic cultivation in China, was performed based on the obtained SSR data.
- Laminaria /
- EST-SSRs /
- germplasmidentification /
- polymorphismanalysis
Abstract: Laminaria (Saccharina) is one of the economic important seaweeds. Through sequence analysis of 4 099 ESTs from Laminaria (971 were generated from our own cDNA laboratories and 3 128 were downloaded from updated EST databases) with SSRIT software, two hundred and fifty-four SSRs in 206 ESTs were found. From the 254 SSRs, sixty-three SSR primer-pairs were designed. In order to test their practicability, the 63 primer-pairs were tested in commonly used SSR reaction conditions using 12 Laminara DNA samples as templates. The results show that 23 SSR primer-pairs gave good amplification patterns on most (more than 80%) of the 12 Laminara DNA templates. Genetic diversity study of 12 Laminaria lines, which were widely used in breeding and economic cultivation in China, was performed based on the obtained SSR data.
- Laminaria /
- EST-SSRs /
- germplasm identification /
- polymorphism analysis

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文章访问数: 384
HTML全文浏览量: 45
PDF下载量: 143
被引次数: 0

Development of EST-SSR primers and their practicability test for Laminaria

doi: 10.1007/s13131-011-0125-4

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Development of EST-SSR primers and their practicability test for Laminaria

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