Isolation via enrichment and characterization of ten polymorphic microsatellite loci in the cuttlefish, <i>Sepiella maindroni</i> de Rochebruns

WU Changwen; CHI Changfeng; HE Guangyuan; L&#220; Zhenming; XU Meiying

doi:10.1007/s13131-010-0083-2

Isolation via enrichment and characterization of ten polymorphic microsatellite loci in the cuttlefish, Sepiella maindroni de Rochebruns

doi: 10.1007/s13131-010-0083-2

1.
Key Laboratory of Molecular Biophysics, Ministry of Education China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, Huazhong University of Science and Technology, Wuhan 430074, China;Marine Science School, Zhejiang Ocean University, Zhoushan 316004, China
2.
Marine Science School, Zhejiang Ocean University, Zhoushan 316004, China;Zhejiang Dahaiyang Science and Technology CO. Ltd., Zhoushan 316004, China
3.
Key Laboratory of Molecular Biophysics, Ministry of Education China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, Huazhong University of Science and Technology, Wuhan 430074, China
4.
Marine Science School, Zhejiang Ocean University, Zhoushan 316004, China

基金项目: The National Key Technology R & D Program of China under contract No. 2007BAD43B05; the Key Science & Technology Specific Projects of Zhejiang Province under contract No. 2007C12062; the National Natural Science Foundation of China under contract No. 31001109

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- 收稿日期: 2009-09-02
- 修回日期: 2010-04-20

Isolation via enrichment and characterization of ten polymorphic microsatellite loci in the cuttlefish, Sepiella maindroni de Rochebruns

1.
Key Laboratory of Molecular Biophysics, Ministry of Education China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, Huazhong University of Science and Technology, Wuhan 430074, China;Marine Science School, Zhejiang Ocean University, Zhoushan 316004, China
2.
Marine Science School, Zhejiang Ocean University, Zhoushan 316004, China;Zhejiang Dahaiyang Science and Technology CO. Ltd., Zhoushan 316004, China
3.
Key Laboratory of Molecular Biophysics, Ministry of Education China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, Huazhong University of Science and Technology, Wuhan 430074, China
4.
Marine Science School, Zhejiang Ocean University, Zhoushan 316004, China

摘要

摘要: Ten novel polymorphic microsatellite loci were developed using an enrichment and colony hybridization protocol and characterized for the cuttlefish, Sepiella maindroni. Polymorphism was explored by using 30 individuals from the coastal waters of Zhanjiang, Guangdong province, China, in December 2007. The number of alleles ranged from 5 to 13. The ranges of observed and expected heterozygosity were from 0.578 9 to 1.000 0 and 0.682 8 to 0.925 7, respectively, and the average polymorphic information content (PIC) was 0.778 5. These microsatellite loci will certainly facilitate the detection of the genetic variation and population structure of S. maindroni.
- Sepiellamaindroni /
- microsatelliteDNA /
- cuttlefish /
- geneticvariation
Abstract: Ten novel polymorphic microsatellite loci were developed using an enrichment and colony hybridization protocol and characterized for the cuttlefish, Sepiella maindroni. Polymorphism was explored by using 30 individuals from the coastal waters of Zhanjiang, Guangdong province, China, in December 2007. The number of alleles ranged from 5 to 13. The ranges of observed and expected heterozygosity were from 0.578 9 to 1.000 0 and 0.682 8 to 0.925 7, respectively, and the average polymorphic information content (PIC) was 0.778 5. These microsatellite loci will certainly facilitate the detection of the genetic variation and population structure of S. maindroni.
- Sepiella maindroni /
- microsatellite DNA /
- cuttlefish /
- genetic variation

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文章访问数: 309
HTML全文浏览量: 32
PDF下载量: 132
被引次数: 0

Isolation via enrichment and characterization of ten polymorphic microsatellite loci in the cuttlefish, Sepiella maindroni de Rochebruns

doi: 10.1007/s13131-010-0083-2

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Isolation via enrichment and characterization of ten polymorphic microsatellite loci in the cuttlefish, Sepiella maindroni de Rochebruns

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