Phylogenetic analysis of aerobic anoxygenic phototrophic bacteria and their relatives based on farnesyl pyrophosphate synthase gene
doi: 10.1007/s13131-010-0066-3
Phylogenetic analysis of aerobic anoxygenic phototrophic bacteria and their relatives based on farnesyl pyrophosphate synthase gene
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摘要: The study aims to reveal phylogenetic and evolutionary relationship between aerobic anoxygenic phototrophic bacteria (AAnPB) and their relatives, anaerobic anoxygenic phototrophic bacteria (AnAnPB) and nonphototrophic bacteria (NPB, which had high homology of 16S rDNA gene with AAnPB and fell into the same genus), and validate reliability and usefulness of farnesyl pyrophosphate synthase (FPPS) gene for the phylogenetic determination. FPPS genes with our modified primers and 16S rDNA genes with general primers, were amplified and sequenced or retrieved from GenBank database. In contrast to 16S rDNA gene phylogenetic tree, AAnPB were grouped into two clusters and one branch alone with no intermingling with NPB and AnAnPB in the tree constructed on FPPS. One branch of AAnPB, in both trees, was located closer to outgroup species than AnAnPB, which implicated that some AAnPB would be diverged earlier in FPPS evolutionary history than AnAnPB and NPB. Some AAnPB and NPB were closer located in both trees and this suggested that they were the closer relatives than AnAnPB. Combination codon usage in FPPS with phylogenetic analysis, the results indicates that FPPS gene and 16S rRNA gene have similar evolutionary pattern but the former seems to be more reliable and useful in determining the phylogenic and evolutionary relationship between AAnPB and their relatives. This is the first attempt to use a molecular marker beside 16S rRNA gene for studying the phylogeny of AAnPB, and the study may also be helpful in understanding the evolutionary relationship among phototrophic microbes and the trends of photosynthetic genes transfer.Abstract: The study aims to reveal phylogenetic and evolutionary relationship between aerobic anoxygenic phototrophic bacteria (AAnPB) and their relatives, anaerobic anoxygenic phototrophic bacteria (AnAnPB) and nonphototrophic bacteria (NPB, which had high homology of 16S rDNA gene with AAnPB and fell into the same genus), and validate reliability and usefulness of farnesyl pyrophosphate synthase (FPPS) gene for the phylogenetic determination. FPPS genes with our modified primers and 16S rDNA genes with general primers, were amplified and sequenced or retrieved from GenBank database. In contrast to 16S rDNA gene phylogenetic tree, AAnPB were grouped into two clusters and one branch alone with no intermingling with NPB and AnAnPB in the tree constructed on FPPS. One branch of AAnPB, in both trees, was located closer to outgroup species than AnAnPB, which implicated that some AAnPB would be diverged earlier in FPPS evolutionary history than AnAnPB and NPB. Some AAnPB and NPB were closer located in both trees and this suggested that they were the closer relatives than AnAnPB. Combination codon usage in FPPS with phylogenetic analysis, the results indicates that FPPS gene and 16S rRNA gene have similar evolutionary pattern but the former seems to be more reliable and useful in determining the phylogenic and evolutionary relationship between AAnPB and their relatives. This is the first attempt to use a molecular marker beside 16S rRNA gene for studying the phylogeny of AAnPB, and the study may also be helpful in understanding the evolutionary relationship among phototrophic microbes and the trends of photosynthetic genes transfer.
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郑重,李少菁,许振祖.海洋浮游生物学[M].北京:海洋出版社,1984:527—546. 刘瑞玉.中国海洋生物名录[M].北京:科学出版社,2008:879—882. THOMPSON H.Pelagic Tunicates of Australia[M].Melbourne: Council for Scientific and Industrial Research.1945:196. 山路勇.日本海洋浮遊生物図鑑[M].大阪:保育社,1977:289—296. TOMITA M, SHIGA N, IKEDA T.Seasonal occurrence and vertical distribution of appendicularians in Toyama Bay, southern Japan Sea[J].Journal of Plankton Research, 2003, 25(6):579—589. ARAVENA G, PALMA S.Taxonomic identification of appendicularians collected in the epipelagic waters off northern Chile (Tunicata, Appendicularia)[J].Revista Chilena de Historia Nature, 2002, 75: 307—325. YANG G F, WANG Y P.Preliminary study on Appendicularia of the northern part of South Sea //Proc on Marine Biology of the South China Sea.Beijing: China Ocean Press,1988:143—153. 黄宗国.中国海洋生物种类与分布[M].北京:海洋出版社,2008:724—725. 陈清潮,张谷贤,陈柏云.西沙、中沙群岛周围海域浮游动物的平面分布和垂直分布 //中国科学院南海海洋研究所.我国西沙、中沙群岛海域海洋生物调查研究报告集.北京:科学出版社,1978:63—72. 陈清潮.南海中部海域浮游生物的初步研究 //中国科学院南海海洋研究所.南海海区综合调查研究报告(一).北京:科学出版社,1982:199—215. 陈清潮.浮游动物 //中国科学院南海海洋研究所.南海海区综合调查研究报告(二).北京:科学出版社,1985:357—379. 韩舞鹰,王明彪,马克美.我国夏季最低表层水温海区——琼东沿岸上升流区的研究[J].海洋与湖沼,1990, 21(3):267—275. 国家海洋局908专项办公室.海洋生物生态调查技术规程[M].北京:海洋出版社,2006:32—35. JING Z Y, QI Y Q, HUA Z L.Numerical study on the summer upwelling system in the northern continental shelf of the South China Sea[J].Continental Shelf Research,2009,29:467—478. -
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