A culture-free method for detection of <i>Vibrio vulnificus</i> from coastal seawater based on loop-mediated isothermal amplification targeting <i>vcgC</i> gene

LI Yongjun; ZHENG Zejun; ZHAO Yulong; WEI Xiaona; ZHU Lin; HUANG Xitai

doi:10.1007/s13131-010-0027-x

A culture-free method for detection of Vibrio vulnificus from coastal seawater based on loop-mediated isothermal amplification targeting vcgC gene

doi: 10.1007/s13131-010-0027-x

1.
The College of Life Sciences, Nankai University, Tianjin 300071, China
2.
The College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China

基金项目: The National High-Tech Research and Development Program of China ("863") under contract No.2006AA09Z170.

计量
- 文章访问数: 383
- HTML全文浏览量: 36
- PDF下载量: 134
- 被引次数: 0
出版历程
- 收稿日期: 2009-01-03
- 修回日期: 2009-08-20

A culture-free method for detection of Vibrio vulnificus from coastal seawater based on loop-mediated isothermal amplification targeting vcgC gene

1.
The College of Life Sciences, Nankai University, Tianjin 300071, China
2.
The College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China

摘要

摘要: Vibrio vulnificus is an opportunistic pathogen widely distributed in estuarine and coastal seawaters. In this study, a culture-free method was developed to rapid detection of V. vulnificus in all seasons, based on loop-mediated isothermal amplification (LAMP) targeting virulent-correlated gene (vcg). The new assay method allows differentiation between the virulent and non-virulent strain of V. vulnificus accurately. This method also allows effective detection of the pathogeny in winter when the bacterium lives in the viable but non-culturable (VBNC) state. A total of 30 costal seawater samples collected in all seasons were used for the evaluation of this method. The results show that the method is sensitive, accurate and convenient.
- Vibriovulnificus /
- LAMP /
- virulence-correlatedgene /
- seawater /
- culture-free /
- VBNC
Abstract: Vibrio vulnificus is an opportunistic pathogen widely distributed in estuarine and coastal seawaters. In this study, a culture-free method was developed to rapid detection of V. vulnificus in all seasons, based on loop-mediated isothermal amplification (LAMP) targeting virulent-correlated gene (vcg). The new assay method allows differentiation between the virulent and non-virulent strain of V. vulnificus accurately. This method also allows effective detection of the pathogeny in winter when the bacterium lives in the viable but non-culturable (VBNC) state. A total of 30 costal seawater samples collected in all seasons were used for the evaluation of this method. The results show that the method is sensitive, accurate and convenient.
- Vibrio vulnificus /
- LAMP /
- virulence-correlated gene /
- seawater /
- culture-free /
- VBNC

HTML全文

参考文献(0)

施引文献

资源附件(0)

访问统计

点击查看大图

计量

文章访问数: 383
HTML全文浏览量: 36
PDF下载量: 134
被引次数: 0

A culture-free method for detection of Vibrio vulnificus from coastal seawater based on loop-mediated isothermal amplification targeting vcgC gene

doi: 10.1007/s13131-010-0027-x

计量

出版历程

A culture-free method for detection of Vibrio vulnificus from coastal seawater based on loop-mediated isothermal amplification targeting vcgC gene

计量

出版历程

目录