Development and characterization of a cell line from the embryos of half smooth tongue sole (Cynoglossus semilaevis)
doi: 10.1007/s13131-010-0025-z
Development and characterization of a cell line from the embryos of half smooth tongue sole (Cynoglossus semilaevis)
-
摘要: A new cell line, CSEC, has been successfully established from embryos at gastrula stage of a cultured marine fish, half smooth tongue sole (Cynoglossus semilaevis). CSEC cells grow actively and stably more than 50 passages for over 200 d in DMEM medium supplemented with 15% FBS (fetal bovine serum), 2.5 ng/cm3 bFGF (basic fibroblast growth factor), 1 ng/cm3 LIF (leukemia inhibitory factor) and 50 mmol/dm3 2-ME (2-mecaptoethanol). The cells grew well in the temperature range of 24-30℃ and the optimal growth temperature was 24℃. FBS and bFGF concentrations are the two key components for CSEC cells proliferation. Chromosome analysis reveals that CSEC cells have a normal diploid karyotype with 2n=42t. The significant fluorescent signals were observed in CSEC after transfection with the GFP reporter gene, suggesting that the CSEC cell line can be used as a useful tool for transgenic and genetic manipulation studies. CSEC cells showed the cytopathic effect (CPE) after infection with lymphosystis disease virus (LCDV) in 2 d. Moreover, the LCDV particles can be observed in the cytoplasm of virus-infected cells by electron microscopy. It suggests that CSEC could be potentially used for the study of aquatic virus.
-
关键词:
- embryoniccellline /
- halfsmoothtonguesole /
- karyotype /
- GFP /
- LCDV /
- CSEC
Abstract: A new cell line, CSEC, has been successfully established from embryos at gastrula stage of a cultured marine fish, half smooth tongue sole (Cynoglossus semilaevis). CSEC cells grow actively and stably more than 50 passages for over 200 d in DMEM medium supplemented with 15% FBS (fetal bovine serum), 2.5 ng/cm3 bFGF (basic fibroblast growth factor), 1 ng/cm3 LIF (leukemia inhibitory factor) and 50 mmol/dm3 2-ME (2-mecaptoethanol). The cells grew well in the temperature range of 24-30℃ and the optimal growth temperature was 24℃. FBS and bFGF concentrations are the two key components for CSEC cells proliferation. Chromosome analysis reveals that CSEC cells have a normal diploid karyotype with 2n=42t. The significant fluorescent signals were observed in CSEC after transfection with the GFP reporter gene, suggesting that the CSEC cell line can be used as a useful tool for transgenic and genetic manipulation studies. CSEC cells showed the cytopathic effect (CPE) after infection with lymphosystis disease virus (LCDV) in 2 d. Moreover, the LCDV particles can be observed in the cytoplasm of virus-infected cells by electron microscopy. It suggests that CSEC could be potentially used for the study of aquatic virus.-
Key words:
- embryonic cell line /
- half smooth tongue sole /
- karyotype /
- GFP /
- LCDV /
- CSEC
-
点击查看大图
计量
- 文章访问数: 362
- HTML全文浏览量: 35
- PDF下载量: 174
- 被引次数: 0
下载:
