2008 Vol. 27, No. 4
Display Method:
2008, (4): 1-16.
Abstract:
After many years' endeavor of research and application practice, the ocean color remote sensing in China has been growing into a new technique with valuable practicality from its initiate stage of trial research. With the aim of operational service, several kinds of ocean color remote sensing application systems have been developed and realized the long-term marine environmental monitoring utilizing the real-time or near real-time satellite and airborne remote sensing data. New progresses in the technology and application of ocean color remote sensing in China are described, including the research of key techniques and the development of various application systems. Meanwhile, according to the application status and demand, the prospective development of Chinese ocean color remote sensing is brought forward. With Chinese second ocean color satellite (HY-1B) orbiting on 11 April 2007 and the development of airborne ocean color remote sensing system for Chinese surveillance planes, great strides will take place in Chinese ocean color remote sensing application with the unique function in marine monitoring, resources management and national security, etc.
After many years' endeavor of research and application practice, the ocean color remote sensing in China has been growing into a new technique with valuable practicality from its initiate stage of trial research. With the aim of operational service, several kinds of ocean color remote sensing application systems have been developed and realized the long-term marine environmental monitoring utilizing the real-time or near real-time satellite and airborne remote sensing data. New progresses in the technology and application of ocean color remote sensing in China are described, including the research of key techniques and the development of various application systems. Meanwhile, according to the application status and demand, the prospective development of Chinese ocean color remote sensing is brought forward. With Chinese second ocean color satellite (HY-1B) orbiting on 11 April 2007 and the development of airborne ocean color remote sensing system for Chinese surveillance planes, great strides will take place in Chinese ocean color remote sensing application with the unique function in marine monitoring, resources management and national security, etc.
2008, (4): 17-25.
Abstract:
On the basis of Argo data and historic temperature/salinity data from the World Ocean Database 2001 (WOD01), origins and spreading pathways of the subsurface and intermediate water masses in the Indonesian Throughflow (ITF) region were discussed by analyzing distributions of salinity on representative isopycnal layers. Results were shown that, subsurface water mostly comes from the North Pacific Ocean while the intermediate water originates from both the North and South Pacific Ocean, even possibly from the Indian Ocean. Spreading through the Sulawesi Sea, the Makassar Strait, and the Flores Sea, the North Pacific subsurface water and the North Pacific Intermediate water dominate the western part of the Indonesian Archipelago. Furthermore as the depth increases, the features of the North Pacific sourced water masses become more obvious. In the eastern part of the waters, high salinity South Pacific subsurface water is blocked by a strong salinity front between Halmahera and New Guinea. Intermediate water in the eastern interior region owns salinity higher than the North Pacific intermediate water and the antarctic intermediate water (AAIW), possibly coming from the vertical mixing between subsurface water and the AAIW from the Pacific Ocean, and possibly coming from the northward extending of the AAIW from the Indian Ocean as well.
On the basis of Argo data and historic temperature/salinity data from the World Ocean Database 2001 (WOD01), origins and spreading pathways of the subsurface and intermediate water masses in the Indonesian Throughflow (ITF) region were discussed by analyzing distributions of salinity on representative isopycnal layers. Results were shown that, subsurface water mostly comes from the North Pacific Ocean while the intermediate water originates from both the North and South Pacific Ocean, even possibly from the Indian Ocean. Spreading through the Sulawesi Sea, the Makassar Strait, and the Flores Sea, the North Pacific subsurface water and the North Pacific Intermediate water dominate the western part of the Indonesian Archipelago. Furthermore as the depth increases, the features of the North Pacific sourced water masses become more obvious. In the eastern part of the waters, high salinity South Pacific subsurface water is blocked by a strong salinity front between Halmahera and New Guinea. Intermediate water in the eastern interior region owns salinity higher than the North Pacific intermediate water and the antarctic intermediate water (AAIW), possibly coming from the vertical mixing between subsurface water and the AAIW from the Pacific Ocean, and possibly coming from the northward extending of the AAIW from the Indian Ocean as well.
2008, (4): 26-38.
Abstract:
The MASNUM wave-tide-circulation coupled model, with 21 layers in the vertical and (1/8)°horizontal resolution, was employed to investigate the oceanic responses to Typhoon Mstsa which traversed the East China Sea (ECS) during the period of 4~6 August, 2005. Numerical experiment results are analyzed and compared with observation. The responses of the sea surface temperature (SST), in a focused area of (27°~29°N, 121°~124°E), include heating and cooling stages. The heating is mainly due to warm Kuroshio water transportation and downwelling due to the water accumulation. In the cooling stage, the amplitude of the simulated cold wake (~3℃), located on the right side of this typhoon track, is compared quite well with that of the satellite observed SST data. The wave-induced mixing(Bv) plays a key role for the SST cooling. Bv still plays a leading role, which accounts for 36%, for the ocean temperature drop in the upper ocean of 0~40 m, while the upwelling is responsible for 84% of the cooling for the lower layer of 40~70 m. The mixed layer depth (MLD) increased quickly from 28 to 50 m in the typhoon period. However, the simulated MLD without the wave-induced vertical mixing, evolution from 13 to 32 m, was seriously underestimated. The surface wave is too important to be ignored for the ocean responses to a typhoon.
The MASNUM wave-tide-circulation coupled model, with 21 layers in the vertical and (1/8)°horizontal resolution, was employed to investigate the oceanic responses to Typhoon Mstsa which traversed the East China Sea (ECS) during the period of 4~6 August, 2005. Numerical experiment results are analyzed and compared with observation. The responses of the sea surface temperature (SST), in a focused area of (27°~29°N, 121°~124°E), include heating and cooling stages. The heating is mainly due to warm Kuroshio water transportation and downwelling due to the water accumulation. In the cooling stage, the amplitude of the simulated cold wake (~3℃), located on the right side of this typhoon track, is compared quite well with that of the satellite observed SST data. The wave-induced mixing(Bv) plays a key role for the SST cooling. Bv still plays a leading role, which accounts for 36%, for the ocean temperature drop in the upper ocean of 0~40 m, while the upwelling is responsible for 84% of the cooling for the lower layer of 40~70 m. The mixed layer depth (MLD) increased quickly from 28 to 50 m in the typhoon period. However, the simulated MLD without the wave-induced vertical mixing, evolution from 13 to 32 m, was seriously underestimated. The surface wave is too important to be ignored for the ocean responses to a typhoon.
2008, (4): 39-56.
Abstract:
For settlement of the well-known problem of contemporary radar imaging models, i. e., the problem of a general underestimation of radar signatures of hydrodynamic features over oceanic internal waves and underwater bottom topography in tidal waters at high radar frequency bands (X-band and C-band), the impact of the ocean surface mixed layer turbulence and the significance of stratified oceanic model on SAR remote sensing of internal solitary waves are proposed. In the north of the South China Sea by utilizing some observed data of background field the nonlinearity coefficient, the dispersion coefficient, the horizontal variability coefficient and the phase speed in the generalized K-dV equation are determined approximately. Through simulations of internal tide transformation the temporal evolution and spatial distribution of the vertical displacement and horizontal velocity of internal wave field are obtained. The simulation results indicate that the maximum amplitudes of internal solitary waves occur at depth 35 m, but the maximum current speeds take place at depth 20 m in this area of the sea (about 20°30'N, 114°E) in August. It was noticed that considering the effects of flood current and ebb current respectively is appropriate to investigate influence of the background shear flow on coefficients of the K-dV equation. The obtained results provide the possibility for the simulation of SAR signatures of internal solitary waves under considering the impact of ocean surface mixed layer turbulence in the companion paper.
For settlement of the well-known problem of contemporary radar imaging models, i. e., the problem of a general underestimation of radar signatures of hydrodynamic features over oceanic internal waves and underwater bottom topography in tidal waters at high radar frequency bands (X-band and C-band), the impact of the ocean surface mixed layer turbulence and the significance of stratified oceanic model on SAR remote sensing of internal solitary waves are proposed. In the north of the South China Sea by utilizing some observed data of background field the nonlinearity coefficient, the dispersion coefficient, the horizontal variability coefficient and the phase speed in the generalized K-dV equation are determined approximately. Through simulations of internal tide transformation the temporal evolution and spatial distribution of the vertical displacement and horizontal velocity of internal wave field are obtained. The simulation results indicate that the maximum amplitudes of internal solitary waves occur at depth 35 m, but the maximum current speeds take place at depth 20 m in this area of the sea (about 20°30'N, 114°E) in August. It was noticed that considering the effects of flood current and ebb current respectively is appropriate to investigate influence of the background shear flow on coefficients of the K-dV equation. The obtained results provide the possibility for the simulation of SAR signatures of internal solitary waves under considering the impact of ocean surface mixed layer turbulence in the companion paper.
2008, (4): 57-62.
Abstract:
The C-band wind speed retrieval models, CMOD4, CMOD-IFR2, and CMOD5 were applied to retrieval of sea surface wind speeds from ENVISAT (European environmental satellite) ASAR (advanced synthetic aperture radar) data in the coastal waters near Hong Kong during a period from October 2005 to July 2007. The retrieved wind speeds are evaluated by comparing with buoy measurements and the QuikSCAT (quick scatterometer) wind products. The results show that the CMOD4 model gives the best performance at wind speeds lower than 15 m/s. The correlation coefficients with buoy and QuikSCAT winds are 0.781 and 0.896, respectively. The root mean square errors are the same 1.74 m/s. Namely, the CMOD4 model is the best one for sea surface wind speed retrieval from ASAR data in the coastal waters near Hong Kong.
The C-band wind speed retrieval models, CMOD4, CMOD-IFR2, and CMOD5 were applied to retrieval of sea surface wind speeds from ENVISAT (European environmental satellite) ASAR (advanced synthetic aperture radar) data in the coastal waters near Hong Kong during a period from October 2005 to July 2007. The retrieved wind speeds are evaluated by comparing with buoy measurements and the QuikSCAT (quick scatterometer) wind products. The results show that the CMOD4 model gives the best performance at wind speeds lower than 15 m/s. The correlation coefficients with buoy and QuikSCAT winds are 0.781 and 0.896, respectively. The root mean square errors are the same 1.74 m/s. Namely, the CMOD4 model is the best one for sea surface wind speed retrieval from ASAR data in the coastal waters near Hong Kong.
2008, (4): 63-72.
Abstract:
Based on new multibeam bathymetric data and about 300 km long single seismic profiles, three topographic units were identified:the canyons, fractural valley and submarine terrace on the north of Chiwei Island where is a structural transition zone between the southern trough and the middle trough. The Chiwei Canyon and the North Chiwei Canyon are two of the largest canyons in the East China Sea (ECS) slope. Topographic features and architectures of them are described. The study shows that both of them are originated along faults. The evolution and spatial distribution of topographic units in the study area are controlled mainly by three groups of faults which were formed and reactive in the recent extensional phase of Okinawa Trough. The Chiwei Canyon was initiated during the middle Pleistocene and guided by F4 that is a N-S trending fault on the slope and F1, a large NW-SE trending fault on the trough. The pathway migration from the remnant channel to the present one of Chiwei Canyon is the result of uplift of tilted fault block that is coupled to the recent extension movements of the southern trough. The submarine terrace is detached from the ECS slope by the NEE-trending fault. The North Chiwei Canyon, developing during the late Pleistocene, is guided by F5, a N-S trending fault, diverted and blocked by the submarine terrace.
Based on new multibeam bathymetric data and about 300 km long single seismic profiles, three topographic units were identified:the canyons, fractural valley and submarine terrace on the north of Chiwei Island where is a structural transition zone between the southern trough and the middle trough. The Chiwei Canyon and the North Chiwei Canyon are two of the largest canyons in the East China Sea (ECS) slope. Topographic features and architectures of them are described. The study shows that both of them are originated along faults. The evolution and spatial distribution of topographic units in the study area are controlled mainly by three groups of faults which were formed and reactive in the recent extensional phase of Okinawa Trough. The Chiwei Canyon was initiated during the middle Pleistocene and guided by F4 that is a N-S trending fault on the slope and F1, a large NW-SE trending fault on the trough. The pathway migration from the remnant channel to the present one of Chiwei Canyon is the result of uplift of tilted fault block that is coupled to the recent extension movements of the southern trough. The submarine terrace is detached from the ECS slope by the NEE-trending fault. The North Chiwei Canyon, developing during the late Pleistocene, is guided by F5, a N-S trending fault, diverted and blocked by the submarine terrace.
2008, (4): 73-82.
Abstract:
Heavy metal concentrations were measured in the Changjiang Estuary and its adjacent waters. Results from a systematic survey in April 2002 to March 2003 indicate that the ranges of the concentrations of dissolved copper, lead, zinc and cadmium in the study waters are 1.01~6.86, 0.10~0.39,3.17~9.12 and 0.011~0.049 μg/dm3, respectively. Similar to zinc, the behavior of dissolved copper was essentially conservative, but high scatter has been observed for high salinity samples, which can be attributed to the decomposition or mineralization of organic matter by bacteria. Dissolved lead may have active behavior with an addition at high salinity. Overall concentrations of dissolved cadmium increase with salinity. The mean values of these dissolved metals calculated for the surface waters were higher than those for the middle and bottom ones. External inputs of dissolved heavy metals to the surface waters were the likely explanation for these higher values. The maximum seasonal average values of dissolved copper and zinc were found in summer, reflecting higher amounts of riverine input in this season. In contrast, the maximum seasonal average values of dissolved lead and copper were found in winter and the lowest ones in summer, respectively, which might be associated with a combination of low concentration with heterogeneous scavenging. Concentrations of these dissolved metals found for the Changjiang Estuary fall in the range observed for the other estuaries but are noticeably higher than those from uncontaminated rivers, except for cadmium. Compared with observations for the Changjiang Estuary in the last two decades, it is clear that the Changjiang estuarine waters has been contaminated with copper, lead, zinc and cadmium during China's industrialization, but concentrations of them have decreased in the last few years.
Heavy metal concentrations were measured in the Changjiang Estuary and its adjacent waters. Results from a systematic survey in April 2002 to March 2003 indicate that the ranges of the concentrations of dissolved copper, lead, zinc and cadmium in the study waters are 1.01~6.86, 0.10~0.39,3.17~9.12 and 0.011~0.049 μg/dm3, respectively. Similar to zinc, the behavior of dissolved copper was essentially conservative, but high scatter has been observed for high salinity samples, which can be attributed to the decomposition or mineralization of organic matter by bacteria. Dissolved lead may have active behavior with an addition at high salinity. Overall concentrations of dissolved cadmium increase with salinity. The mean values of these dissolved metals calculated for the surface waters were higher than those for the middle and bottom ones. External inputs of dissolved heavy metals to the surface waters were the likely explanation for these higher values. The maximum seasonal average values of dissolved copper and zinc were found in summer, reflecting higher amounts of riverine input in this season. In contrast, the maximum seasonal average values of dissolved lead and copper were found in winter and the lowest ones in summer, respectively, which might be associated with a combination of low concentration with heterogeneous scavenging. Concentrations of these dissolved metals found for the Changjiang Estuary fall in the range observed for the other estuaries but are noticeably higher than those from uncontaminated rivers, except for cadmium. Compared with observations for the Changjiang Estuary in the last two decades, it is clear that the Changjiang estuarine waters has been contaminated with copper, lead, zinc and cadmium during China's industrialization, but concentrations of them have decreased in the last few years.
2008, (4): 83-94.
Abstract:
Distributions and sources of total organic carbon(TOC)in seabed sediments and their implications for hydrodynamics are analyzed, in the turbidity maximum of the Changjiang Estuary. Ecology ecoenvironmental effects of estuary water on the continuously increasing terrigenous organic carbon from the Changjiang River are also explored through variations of organic carbon content and water quality indicators. Results show that, hydrodynamics exert important influences on distributions of organic carbon in the turbidity maximum of Changjiang Estuary. For their redistribution effect of terrigenous organic carbon within the moving layer in the whole region, variations from land to sea are not indicated by surficial and vertical average values of TOC and total nitrogen (TN) contents in core sediment, as well as organic stable carbon isotopes in surface sediments. However, on the long-time scale, the trend of terrigenous organic carbon decreasing from land to sea is still displayed by variations of stable carbon isotopic average values becoming heavier from land to sea. Previous studies have shown that high content of Chl a cannot appear in the Changjiang Estuary in despite of adequate nourishment supply, because photosynthesis of phytoplankton is constrained by high suspended sediment concentration(SSC). However, an area with a high content of Chl a occurs, which may be caused by resuspended benthic algae with bottom fine grain-size sediments. Tremendous pressures are imposed on the environment of Changjiang Estuary, because of ultrophication trends and special hydrodynamics. Phytoplankton bloom area tends to extend from the outer sea to the mouth of Changjiang River.
Distributions and sources of total organic carbon(TOC)in seabed sediments and their implications for hydrodynamics are analyzed, in the turbidity maximum of the Changjiang Estuary. Ecology ecoenvironmental effects of estuary water on the continuously increasing terrigenous organic carbon from the Changjiang River are also explored through variations of organic carbon content and water quality indicators. Results show that, hydrodynamics exert important influences on distributions of organic carbon in the turbidity maximum of Changjiang Estuary. For their redistribution effect of terrigenous organic carbon within the moving layer in the whole region, variations from land to sea are not indicated by surficial and vertical average values of TOC and total nitrogen (TN) contents in core sediment, as well as organic stable carbon isotopes in surface sediments. However, on the long-time scale, the trend of terrigenous organic carbon decreasing from land to sea is still displayed by variations of stable carbon isotopic average values becoming heavier from land to sea. Previous studies have shown that high content of Chl a cannot appear in the Changjiang Estuary in despite of adequate nourishment supply, because photosynthesis of phytoplankton is constrained by high suspended sediment concentration(SSC). However, an area with a high content of Chl a occurs, which may be caused by resuspended benthic algae with bottom fine grain-size sediments. Tremendous pressures are imposed on the environment of Changjiang Estuary, because of ultrophication trends and special hydrodynamics. Phytoplankton bloom area tends to extend from the outer sea to the mouth of Changjiang River.
2008, (4): 95-111.
Abstract:
The spatial and temporal characteristics of trophic structure of fish communities in the southern Huanghai Sea were examined based on the data sampled from bottom trawl surveys conducted during the autumn of 2000 and the spring of 2001. Hierarchical agglomerative cluster method and bootstrap randomization were used to identify significant trophic groups for each fish assemblage in the southern Huanghai Sea. A total of six major trophic groups were identified within this system, which classified predators based upon location in the water column or prey size (i. e., benthic to pelagic predators or fish to small invertebrate prey predators). The similarity level used to identify significant trophic groups in each assemblage ranged from 24% to 34%. Although planktivores were the dominant trophic group in each assemblage (60%~79%), there were spatial and temporal variations in the trophic structure, which reflected the differences in the abundance and availability of dominant preys. Simplified food webs were constructed to evaluate the most important trophic relationships between the dominant prey taxa and the fishes in each assemblage within this system. Although there were some differences in the key prey species among different food webs, pelagic prey items (mainly euphausiids and copepods) represent the most important energetic link between primary producers and higher trophic level predators. The trophic level for most fishes was between 3 and 4, and the weighted mean trophic level for each assemblage ranged from 3.3 to 3.4. Compared with previous study in the mid-1980s, there was an obvious downward trend in the trophic level for most fish species, which resulted mainly from the fluctuation in key prey species in the Huanghai Sea. The decrease in the importance of Japanese anchovy seems to be offset by other abundant prey species such as Euphausia pacif ica and copepods (mainly Calanus sinicus).
The spatial and temporal characteristics of trophic structure of fish communities in the southern Huanghai Sea were examined based on the data sampled from bottom trawl surveys conducted during the autumn of 2000 and the spring of 2001. Hierarchical agglomerative cluster method and bootstrap randomization were used to identify significant trophic groups for each fish assemblage in the southern Huanghai Sea. A total of six major trophic groups were identified within this system, which classified predators based upon location in the water column or prey size (i. e., benthic to pelagic predators or fish to small invertebrate prey predators). The similarity level used to identify significant trophic groups in each assemblage ranged from 24% to 34%. Although planktivores were the dominant trophic group in each assemblage (60%~79%), there were spatial and temporal variations in the trophic structure, which reflected the differences in the abundance and availability of dominant preys. Simplified food webs were constructed to evaluate the most important trophic relationships between the dominant prey taxa and the fishes in each assemblage within this system. Although there were some differences in the key prey species among different food webs, pelagic prey items (mainly euphausiids and copepods) represent the most important energetic link between primary producers and higher trophic level predators. The trophic level for most fishes was between 3 and 4, and the weighted mean trophic level for each assemblage ranged from 3.3 to 3.4. Compared with previous study in the mid-1980s, there was an obvious downward trend in the trophic level for most fish species, which resulted mainly from the fluctuation in key prey species in the Huanghai Sea. The decrease in the importance of Japanese anchovy seems to be offset by other abundant prey species such as Euphausia pacif ica and copepods (mainly Calanus sinicus).
2008, (4): 112-119.
Abstract:
A2×2 factorial cross between two populations of Pacific abalone Haliotis discus hannai Ino, collected separately from Dalian (D) in China and Miyagi (M) in Japan, was conducted to compare performances in fertilization rate, hatching rate, metamorphosis rate and growth at days 20, 43, 160 and 330 between purebreds (DD and MM) and crossbreds (DM and MD) and investigate the magnitude of heterobeltiosis (better parent) and heterosis (mid-parent). Heterobeltiosis and heterosis for all the traits analyzed were evidently different between crossbreds DM and MD. Heterobeltiosis in the crossbred DM varied among traits, with values of 2.5% for the fertilization rate, 2.2% for the hatching rate,-1.9% for the metamorphosis rate and 7.4% for the growth at the day 330. The crossbred DM displayed positive heterotic values for fertilization rate (5.4%), hatching rate (7.4%), metamorphosis rate (7.6%) and growth (12.0%) at the day 330. However, both heterobeltiosis and heterosis for all the traits in the crossbred MD were negative except those for the growth at days 20 and 43. The results indicate the importance of selecting superior hybrid varieties if the exploitation of hybrid vigor is considered in the Pacific abalone breeding program.
A2×2 factorial cross between two populations of Pacific abalone Haliotis discus hannai Ino, collected separately from Dalian (D) in China and Miyagi (M) in Japan, was conducted to compare performances in fertilization rate, hatching rate, metamorphosis rate and growth at days 20, 43, 160 and 330 between purebreds (DD and MM) and crossbreds (DM and MD) and investigate the magnitude of heterobeltiosis (better parent) and heterosis (mid-parent). Heterobeltiosis and heterosis for all the traits analyzed were evidently different between crossbreds DM and MD. Heterobeltiosis in the crossbred DM varied among traits, with values of 2.5% for the fertilization rate, 2.2% for the hatching rate,-1.9% for the metamorphosis rate and 7.4% for the growth at the day 330. The crossbred DM displayed positive heterotic values for fertilization rate (5.4%), hatching rate (7.4%), metamorphosis rate (7.6%) and growth (12.0%) at the day 330. However, both heterobeltiosis and heterosis for all the traits in the crossbred MD were negative except those for the growth at days 20 and 43. The results indicate the importance of selecting superior hybrid varieties if the exploitation of hybrid vigor is considered in the Pacific abalone breeding program.
2008, (4): 120-129.
Abstract:
To get a better understanding of the starvation survival strategy of pathogenic Vibrio alginolyticus, log-phase cells were inoculated into sterile natural seawater for starvation studies. The results showed that all of total bacteria number, viable bacteria number and CFU number of V. alginolyticus increased remarkably at the initial starvation stage; after reaching their peaks at 5 d, both total bacteria number and viable bacteria number of V. alginolyticus fell slowly, while the CFU number fell more quickly after reaching its peak at 10 d; V. alginolyticus elongated their cells at the prophase of starvation, and then shrunk their volume and turned their shapes into ovals from rods at the anaphase of starvation; starved cells showed more sensitivity to heating and UV; starved cells showed no significant difference from unstarved ones at the lowest detection limit determined by indirect enzyme-linked immunosorbent assay (ELISA); starved cells' ability to adhere to the skin mucus of large yellow croaker (Pseudosciaena crocea) showed a sharp decline as the starvation time increases; the cellular protein of V. alginolyticus increased remarkably at the anaphase of starvation. The results indicated that pathogenic V. alginolyticus could survive in starvation for relatively long periods of time (≥ 2 months) in 28℃ natural seawater due to the morphological and physiological changes; however, starved V. alginolyticus cells showed less virulence and higher sensitivity under environmental stresses.
To get a better understanding of the starvation survival strategy of pathogenic Vibrio alginolyticus, log-phase cells were inoculated into sterile natural seawater for starvation studies. The results showed that all of total bacteria number, viable bacteria number and CFU number of V. alginolyticus increased remarkably at the initial starvation stage; after reaching their peaks at 5 d, both total bacteria number and viable bacteria number of V. alginolyticus fell slowly, while the CFU number fell more quickly after reaching its peak at 10 d; V. alginolyticus elongated their cells at the prophase of starvation, and then shrunk their volume and turned their shapes into ovals from rods at the anaphase of starvation; starved cells showed more sensitivity to heating and UV; starved cells showed no significant difference from unstarved ones at the lowest detection limit determined by indirect enzyme-linked immunosorbent assay (ELISA); starved cells' ability to adhere to the skin mucus of large yellow croaker (Pseudosciaena crocea) showed a sharp decline as the starvation time increases; the cellular protein of V. alginolyticus increased remarkably at the anaphase of starvation. The results indicated that pathogenic V. alginolyticus could survive in starvation for relatively long periods of time (≥ 2 months) in 28℃ natural seawater due to the morphological and physiological changes; however, starved V. alginolyticus cells showed less virulence and higher sensitivity under environmental stresses.
2008, (4): 130-136.
Abstract:
White spot syndrome virus (WSSV) is one of the major shrimp pathogens causing large economic losses to shrimp farming. In an attempt to identify the envelope proteins involved in the virus infection, purified WSSV virions were mixed with three antisera against WSSV envelope proteins (VP39, VP124 and VP187), individually. And then they were injected intramuscularly into crayfish (Procambarus clarkii) to conduct in vivo neutralization assays. The results showed that for groups injected with virions only and groups injected with the mixture of virions and antiserum against VP124, the crayfish mortalities were 100% and 60% on the 8th day postinfection, individually. The virus infection could be delayed or neutralized by antibody against the envelope protein VP124. Quantitative PCR was used to further investigate the influence of three antisera described above on the virus infection. The results showed that the antiserum against VP124 could restrain the propagation of WSSV in crayfish. All of the results suggested that the viral envelope protein VP124 played a role in WSSV infection.
White spot syndrome virus (WSSV) is one of the major shrimp pathogens causing large economic losses to shrimp farming. In an attempt to identify the envelope proteins involved in the virus infection, purified WSSV virions were mixed with three antisera against WSSV envelope proteins (VP39, VP124 and VP187), individually. And then they were injected intramuscularly into crayfish (Procambarus clarkii) to conduct in vivo neutralization assays. The results showed that for groups injected with virions only and groups injected with the mixture of virions and antiserum against VP124, the crayfish mortalities were 100% and 60% on the 8th day postinfection, individually. The virus infection could be delayed or neutralized by antibody against the envelope protein VP124. Quantitative PCR was used to further investigate the influence of three antisera described above on the virus infection. The results showed that the antiserum against VP124 could restrain the propagation of WSSV in crayfish. All of the results suggested that the viral envelope protein VP124 played a role in WSSV infection.
2008, (4): 137-146.
Abstract:
Laminaria japonica, Undaria pinnatif ida, Ulva lactuca, Grateloupia turuturu and Palmaria palmata are suitable species that fit the requirements of a seaweed-animal integrated aquaculture system in terms of their viable biomass, rapid growth and promising nutrient uptake rates. In this investigation, the responses of the optimal chlorophyll fluorescence yield of the five algal species in tumble culture were assessed at a temperature range of 10~30℃. The results revealed that Ulva lactuca was the most resistant species to high temperature, withstanding 30℃ for 4 h without apparent decline in the optimal chlorophyll fluorescence yield. While the arctic alga Palmaria palmata was the most vulnerable one, showing significant decline in the optimal chlorophyll fluorescence yield at 25℃ for 2 h. The cold-water species Laminaria japonica, however, demonstrated strong ability to cope with higher temperature (24~26℃) for shorter time (within 24 h) without significant decline in the optimal chlorophyll fluorescence yield. Grateloupia turuturu showed a general decrease in the optimal chlorophyll fluorescence yield with the rising temperature from 23 to 30℃, similar to the temperate kelp Undaria pinnatifida. Changes of chlorophyll fluorescence yields of these algae were characterized differently indicating the existence of species-unique strategy to cope with high light. Measurements of the optimal chlorophyll fluorescence yield after short exposure to direct solar irradiance revealed how long these exposures could be without significant photoinhibition or with promising recovery in photosynthetic activities. Seasonal pattern of alternation of algal species in tank culture in the Northern Hemisphere at the latitude of 36°N was proposed according to these basic measurements.
Laminaria japonica, Undaria pinnatif ida, Ulva lactuca, Grateloupia turuturu and Palmaria palmata are suitable species that fit the requirements of a seaweed-animal integrated aquaculture system in terms of their viable biomass, rapid growth and promising nutrient uptake rates. In this investigation, the responses of the optimal chlorophyll fluorescence yield of the five algal species in tumble culture were assessed at a temperature range of 10~30℃. The results revealed that Ulva lactuca was the most resistant species to high temperature, withstanding 30℃ for 4 h without apparent decline in the optimal chlorophyll fluorescence yield. While the arctic alga Palmaria palmata was the most vulnerable one, showing significant decline in the optimal chlorophyll fluorescence yield at 25℃ for 2 h. The cold-water species Laminaria japonica, however, demonstrated strong ability to cope with higher temperature (24~26℃) for shorter time (within 24 h) without significant decline in the optimal chlorophyll fluorescence yield. Grateloupia turuturu showed a general decrease in the optimal chlorophyll fluorescence yield with the rising temperature from 23 to 30℃, similar to the temperate kelp Undaria pinnatifida. Changes of chlorophyll fluorescence yields of these algae were characterized differently indicating the existence of species-unique strategy to cope with high light. Measurements of the optimal chlorophyll fluorescence yield after short exposure to direct solar irradiance revealed how long these exposures could be without significant photoinhibition or with promising recovery in photosynthetic activities. Seasonal pattern of alternation of algal species in tank culture in the Northern Hemisphere at the latitude of 36°N was proposed according to these basic measurements.
2008, (4): 147-155.
Abstract:
Carbon,nitrogen,phosphorus,silicon composition of cultured two different sized phytoplankton common species of Thalassiosira rotula and Skeletonema costatum from the Jiaozhou Bay were measured. Carbon,nitrogen,phosphorus,silicon contents in cell were obvious higher in T. rotula than in S. costatum, but the percents of nitrogen,phosphorus,silicon contents in cell dry mass in T. rotula were lower than those in S. costatum. The dry mass concentrations of nitrogen,phosphorus,silicon in S. costatum were much higher than those in T. rotula, particularly silicon, the former was 6.4 times of the latter, showing that S. costatum could more assimilate these elements. Especially, S. costatum had competitive dominance for assimilating silicon, which is beneficial to its becoming a major dominant species in relative short silicon of the Jiaozhou Bay. There were some differences in numerical value of nutrient ratios both laboratory-cultured phytoplankton and different sized suspended particulates (mainly phytoplankton) in the Jiaozhou Bay, which was caused by the changes of environment. High contents of carbon, nitrogon and relative low phosporus,silicon, high molar ratio of nitrogen to phosphorus (far higher than Redfield value) and low ratio of silicon to phosphorus and silicon to nitrogen (far lower than Redfield values) in the two diatoms and different sized suspended particulates were consistent with those in the seawater. Relative short silicon in the seawater and phytoplankton showed that silicon was possibly affectting phytoplankton growth in the Jiaozhou Bay.
Carbon,nitrogen,phosphorus,silicon composition of cultured two different sized phytoplankton common species of Thalassiosira rotula and Skeletonema costatum from the Jiaozhou Bay were measured. Carbon,nitrogen,phosphorus,silicon contents in cell were obvious higher in T. rotula than in S. costatum, but the percents of nitrogen,phosphorus,silicon contents in cell dry mass in T. rotula were lower than those in S. costatum. The dry mass concentrations of nitrogen,phosphorus,silicon in S. costatum were much higher than those in T. rotula, particularly silicon, the former was 6.4 times of the latter, showing that S. costatum could more assimilate these elements. Especially, S. costatum had competitive dominance for assimilating silicon, which is beneficial to its becoming a major dominant species in relative short silicon of the Jiaozhou Bay. There were some differences in numerical value of nutrient ratios both laboratory-cultured phytoplankton and different sized suspended particulates (mainly phytoplankton) in the Jiaozhou Bay, which was caused by the changes of environment. High contents of carbon, nitrogon and relative low phosporus,silicon, high molar ratio of nitrogen to phosphorus (far higher than Redfield value) and low ratio of silicon to phosphorus and silicon to nitrogen (far lower than Redfield values) in the two diatoms and different sized suspended particulates were consistent with those in the seawater. Relative short silicon in the seawater and phytoplankton showed that silicon was possibly affectting phytoplankton growth in the Jiaozhou Bay.
2008, (4): 156-164.
Abstract:
The germplasm of breeding large yellow croaker (Pseudosciaena crocea Richardson) was revealed using 12 microsatellite markers. The results showed that the genetic diversities were on a mediated level in the bred Daiqu and Min-Yue stocks and two hybrid groups, as represented by 4.83 of the mean number of alleles and 0.561 of the average observed heterozygosity. The value of pairwise differentiation coefficient(FST) was only 13.1% between Daiqu and Min-Yue stocks, demonstrating the low level of differentiation and a close relationship. However, STRUCTURE simulations and phylogenetic tree based on the UPGMA method supported that they are geographically different populations of the same species with distinct genetic structures. Examinations of individual admixture showed that Min-Yue stock had been contaminated by alien individuals. Moreover, the genetic structures of the two hybrid groups resembled those of their parents, especially affected more by their female parents. Finally, the values of average observed heterozygosity between parents and their ascendants were compared and tested, as a result of no detectable differences (P > 0.05).
The germplasm of breeding large yellow croaker (Pseudosciaena crocea Richardson) was revealed using 12 microsatellite markers. The results showed that the genetic diversities were on a mediated level in the bred Daiqu and Min-Yue stocks and two hybrid groups, as represented by 4.83 of the mean number of alleles and 0.561 of the average observed heterozygosity. The value of pairwise differentiation coefficient(FST) was only 13.1% between Daiqu and Min-Yue stocks, demonstrating the low level of differentiation and a close relationship. However, STRUCTURE simulations and phylogenetic tree based on the UPGMA method supported that they are geographically different populations of the same species with distinct genetic structures. Examinations of individual admixture showed that Min-Yue stock had been contaminated by alien individuals. Moreover, the genetic structures of the two hybrid groups resembled those of their parents, especially affected more by their female parents. Finally, the values of average observed heterozygosity between parents and their ascendants were compared and tested, as a result of no detectable differences (P > 0.05).
