Time series measurements (2010–2017) from the Research Moored Array for African–Asian–Australian Monsoon Analysis and Prediction (RAMA) moorings at 15°N, 90°E and 12°N, 90°E are used to investigate the effect of the seasonal barrier layer (BL) on the mixed-layer heat budget in the Bay of Bengal (BoB). The mixed-layer temperature tendency (
$\partial T/\partial t $
) is primarily controlled by the net surface heat flux that remains in the mixed layer (
$ {Q}^{\text{'}} $
) from March to October, while both
$ {Q}^{\text{'}} $
and the vertical heat flux at the base of the mixed layer (
$ {Q}_{h} $
), estimated as the residual of the mixed-layer heat budget, dominate during winter (November–February). An inverse relation is observed between the BL thickness and the mixed-layer temperature (
$ \mathrm{M}\mathrm{L}\mathrm{T} $
). Based on the estimations at the moorings, it is suggested that when the BL thickness is ≥25 m, it exerts a considerable influence on
$\partial T/\partial t$
through the modulation of
$ {Q}_{h} $
(warming) in the BoB. The cooling associated with
$ {Q}_{h} $
is strongest when the BL thickness is ≤10 m with the
$ \mathrm{M}\mathrm{L}\mathrm{T} $
exceeding 29°C, while the contribution from
$ {Q}_{h} $
remains nearly zero when the BL thickness varies between 10 m and 25 m. Temperature inversion is evident in the BoB during winter when the BL thickness remains ≥25 m with an average
$ \mathrm{M}\mathrm{L}\mathrm{T} $
<28.5°C. Furthermore,
$ {Q}_{h} $
follows the seasonal cycle of the BL at these RAMA mooring locations, with
$ r > 0.72 $
at the 95% significance level.